Recombinant Human Fibronectin Fragment 4 Protein, CF

Catalog #: 3624-FN
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
3624-FN-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Fibronectin Fragment 4 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED₅₀ for this effect is 0.05-0.35 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human Fibronectin protein
Val1913-Glu2477, with a C-terminal 6-His tag

Accession #

NP_997647

N-terminal Sequence
Analysis

Val1913

Predicted Molecular Mass

63.5 kDa

SDS-PAGE

70-95 kDa, reducing conditions

Product Datasheets

3624-FN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3624-FN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Fibronectin

Fibronectin (FN) is a large modular glycoprotein that is found in a polymeric fibrillar network in the extracellular matrix (ECM). It also forms a soluble disulfide-linked dimeric protomers in plasma and other body fluids (1,2). The protein subunit is made up of three types of homologous structural motifs termed FN type I, type II, and type III repeats (3-5). Alternative splicing generates multiple isoforms of fibronectin which may have insertions of extra type III domains (EDA and EDB) or alteration of the type III connecting segment (IIICS) (5). Fibronectin is a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, and integrins. It is involved in multiple cellular processes including cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils in the ECM. Conversion of soluble fibronectin to fibronectin fibrils in the ECM is initiated by binding to cell surface integrins, resulting in exposure of cryptic epitopes necessary for polymerization (1). FN1.4 contains one type III domain, the IIICS domain, three type I domains, and the site of interchain disulfide linkage. Within FN1.4, human fibronectin shares 91% and 88% aa sequence identity with mouse and rat fibronectin, respectively. FN1.4 contains regions that enable association with heparin and fibrin. The IIICS domain contains two sites (CS1 and CS2) that interact with integrin alpha 4 beta 1 (6-8). The CS1 sequence is not accessible in full length fibronectin but is exposed by protease digestion, thereby enabling cell adhesion via integrin alpha 4 beta 1 (9). This is distinct from integrin alpha 5 beta 1-mediated adhesion through an RGD motif located N-terminal to FN1.4 (10). Differential splicing within the IIICS domain determines the presence of CS1 and CS2 sequences and the sensitivity to proteases (6,11).

References

Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
Potts, J.R. and I.D. Campbell (1996) Matrix Biol. 15:313.
Bernard, M.P. et al. (1985) Biochemistry 24:2698.
Kornblihtt, A.R. et al. (1983) Proc. Natl. Acad. Sci. 80:3218.
Kornblihtt, A.R. et al. (1985) EMBO J. 4:1755.
Mould, A.P. et al. (1991) J. Biol. Chem. 266:3579.
Komoriya, A., et al. (1991) J. Biol. Chem. 266:15075.
Wayner, E.A., et al. (1989) J. Cell Biol. 109:1321.
Ugarova, T.P. et al. (1996) Biochemistry 35:10913.
Pytela, R. et al. (1985) Cell 40:191.
Abe, Y. et al. (2005) Biochem. Biophys. Res. Commun. 338:1640.

Entrez Gene IDs

2335 (Human); 280794 (Bovine)

Alternate Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF

Related Research Areas

Citation for Recombinant Human Fibronectin Fragment 4 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Dermatopontin interacts with fibronectin, promotes fibronectin fibril formation, and enhances cell adhesion.
Authors: Kato A, Okamoto O, Ishikawa K, Sumiyoshi H, Matsuo N, Yoshioka H, Nomizu M, Shimada T, Fujiwara S
J. Biol. Chem., 2011-03-11;286(17):14861-9.
Species: Human
Sample Types:
Applications: Bioassay