Home / Follistatin / Recombinant Human Follistatin 288 (FS-288) Protein

Recombinant Human Follistatin 288 (FS-288) Protein

Catalog #: 5836-FS
10 Citations Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
5836-FS-025/CF

With Carrier

Catalog # Availability Size / Price Qty
5836-FS-025
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (10)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Follistatin 288 (FS-288) Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells.

The ED50 for this effect is 0.05‑0.25 μg/mL in the presence of 7.5 ng/mL of rhActivin A.

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Follistatin protein
Gly30-Asn317, with an N-terminal Met
Accession #
P19883
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
31.7 kDa
SDS-PAGE
35-40 kDa, reducing conditions

Product Datasheets

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5836-FS (with carrier)

Product Datasheet
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5836-FS/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5836-FS

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

5836-FS/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Follistatin

Follistatin 288 (FST288) is one of three forms of follistatin, a secreted glycoprotein that was first identified as a follicle-stimulating hormone inhibiting substance in ovarian follicular fluid (1, 2). Human follistatin 288 cDNA encodes a 317 amino acid (aa) protein with a 29 aa signal sequence, and a 288 aa mature region. Human FST288 shares 97 - 99% aa identity with corresponding regions of mouse, rat, equine, ovine, porcine and bovine FST. All forms of follistatin, including FST288, FST303 and FST315, contain an N-terminal atypical TGF binding domain and three follistatin domains (FS1 - 3) that contain EGF-like and kazal-like motifs. A highly acidic C-terminal tail is missing in the splice variant FST288, partially present in the proteolytically produced FST303, and full-length in the most abundant form, FST315 (5, 8, 9). FSTs inhibit activins by surrounding activin dimers. FST288 shows the highest affinity for activins due to its extended configuration, while FST315 is in a folded form (2, 8). Expression of the isoforms is restricted such that FST288, which has higher affinity for heparin-sulfated proteoglycans when unbound, is present in tissues while FST315 is the sole form in plasma and ovarian follicular fluid (5, 8, 9). In addition to activin, follistatins regulate the bioavailability of other members of the TGF-beta superfamily, such as BMP6, BMP7 and myostatin (5). Follistatins also regulate hematopoietic stem cell adhesion to fibronectin via FS2, and bind angiogenin via FS2 and FS3 (6, 7). Genetic deletion of follistatin in mice, or expression of only the FST288 form, is perinatally lethal due to defects of lung, skin and musculoskeletal system (10). Mice that express only the FST315 isoform survive, but exhibit defects in vascularization and female fertility (10).

References
  1. Shimasaki, S. et al. (1988) Proc. Natl. Acad. Sci. USA 85:4218. 
  2. Thompson, T.B. et al. (2005) Dev. Cell 9:535. 
  3. Sidis, Y. et al. (2005) Endocrinology 146:130. 
  4. Keutmann, H.T. et al. (2004) Mol. Endocrinol. 18:228. 
  5. Sidis, Y. et al. (2006) Endocrinology 147:3586. 
  6. Maguer-Satta, V. et al. (2006) Exp. Cell Res. 312:434.
  7. Gao, X. et al. (2007) FEBS Lett. 581:5505.
  8. Lerch, T.F. et al. (2007) J. Biol. Chem. 282:15930.
  9. Schneyer, A.L. et al. (2004) J. Clin. Endocrinol. Metab. 89:5067.
  10. Lin, S-Y. et al. (2008) Mol. Endocrinol. 22:415.
Entrez Gene IDs
10468 (Human); 14313 (Mouse)
Alternate Names
follistatin isoform FST317; Follistatin; FS; FSActivin-binding protein; FST

Citations for Recombinant Human Follistatin 288 (FS-288) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Activin A Limits VEGF-Induced Permeability via VE-PTP
    Authors: Baccouche, B;Lietuvninkas, L;Kazlauskas, A;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Follistatin Is a Novel Chemoattractant for Migration and Invasion of Placental Trophoblasts of Mice
    Authors: J Li, Y Qi, K Yang, L Zhu, X Cui, Z Liu
    Cells, 2022-11-29;11(23):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Role of stromal activin A in human pancreatic cancer and metastasis in mice
    Authors: G Mancinelli, C Torres, N Krett, J Bauer, K Castellano, R McKinney, D Dawson, G Guzman, R Hwang, S Grimaldo, P Grippo, B Jung
    Scientific Reports, 2021-04-12;11(1):7986.
    Species: Human
    Sample Types: Protein
    Applications: Reference Standard
  4. Increased stiffness of the tumor microenvironment in colon cancer stimulates cancer associated fibroblast-mediated prometastatic activin A signaling
    Authors: J Bauer, MAB Emon, JJ Staudacher, AL Thomas, J Zessner-Sp, G Mancinelli, N Krett, MT Saif, B Jung
    Sci Rep, 2020-01-09;10(1):50.
    Species: Human
    Sample Types: cell culture
    Applications: Cell Culture
  5. Activin in acute pancreatitis: Potential risk-stratifying marker and novel therapeutic target.
    Authors: Jonas J Staudacher, Cemal Yazici, Timothy Carroll, Jessica Bauer, Jingbo Pang, Nancy Krett, Yinglin Xia, Annette Wilson, Georgios Papachris, Andrea Dirmeier, Claudia Kunst, David C Whitcomb, Giamila Fantuzzi, Barbara Jung
    Scientific Reports, 2017-10-06;0(0):2045-2322.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Activin signaling is an essential component of the TGF-? induced pro-metastatic phenotype in colorectal cancer
    Authors: JJ Staudacher, J Bauer, A Jana, J Tian, T Carroll, G Mancinelli, Ö Özden, N Krett, G Guzman, D Kerr, P Grippo, B Jung
    Sci Rep, 2017-07-17;7(1):5569.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Structural basis for potency differences between GDF8 and GDF11
    Authors: RG Walker, M Czepnik, EJ Goebel, JC McCoy, A Vujic, M Cho, J Oh, S Aykul, KL Walton, G Schang, DJ Bernard, AP Hinck, CA Harrison, E Martinez-H, AJ Wagers, RT Lee, TB Thompson
    BMC Biol, 2017-03-03;15(1):19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Patient-specific hepatocyte-like cells derived from induced pluripotent stem cells model pazopanib-mediated hepatotoxicity
    Authors: Y Choudhury, YC Toh, J Xing, Y Qu, J Poh, L Huan, HS Tan, R Kanesvaran, H Yu, MH Tan
    Sci Rep, 2017-01-25;7(0):41238.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Follistatin in chondrocytes: the link between TRPV4 channelopathies and skeletal malformations.
    Authors: Leddy H, McNulty A, Lee S, Rothfusz N, Gloss B, Kirby M, Hutson M, Cohn D, Guilak F, Liedtke W
    FASEB J, 2014-02-27;28(6):2525-37.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: Bioassay
  10. The role of the activin system in keloid pathogenesis.
    Authors: Mukhopadhyay A, Chan SY, Lim IJ, Phillips DJ, Phan TT
    Am. J. Physiol., Cell Physiol., 2006-09-13;292(4):C1331-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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