Recombinant Human Follistatin-like 5/FSTL5 Protein, CF

Catalog #: 9066-FN Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9066-FN-100

Recombinant Human Follistatin-like 5/FSTL5 Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Follistatin-like 5/FSTL5 Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Hep3B human hepatocellular carcinoma cell survival. The ED₅₀ for this effect is 3-12 μg/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human Follistatin-like 5/FSTL5 protein
Arg21-Ala847, with a C-terminal 6-His tag

Accession #

AAH36502

N-terminal Sequence
Analysis

Arg21

Predicted Molecular Mass

94 kDa

SDS-PAGE

86-102 kDa, reducing conditions

Product Datasheets

9066-FN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9066-FN

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human Follistatin-like 5/FSTL5 (Catalog # 9066-FN) inhibits Hep3B human hepatocellular carcinoma cell survival. The ED₅₀ for this effect is 3-12 μg/mL.

Reconstitution Calculator

Background: Follistatin-like 5/FSTL5

Follistatin-like 5 (FSTL5) is a secreted 94 kDa (predicted) member of the SPARC protein family (1). In its N-terminal half, FSTL5 contains a Kazal-like domain,
two EF-hand domains, and two Ig-like domains. Mature human FSTL5 shares 91% amino acid sequence identity with mouse and rat FSTL5. Alternative splicing generates an isoform with a 10 residue deletion following the second Ig-like domain. FSTL5 is expressed in the cartilage and tendon of developing digits and the shafts of long bones (2). In the brain it is expressed in the olfactory bulb, CA3 area of the hippocampus, and granule cell layer of the cerebellum (3). FSTL5 inhibits the proliferation and promotes the apoptosis of hepatocellular carcinoma cells (4). Its expression in medulloblastoma and down-regulation in hepatocellular carcinoma are associated with poor prognosis (4, 5).

References