Recombinant Human Frizzled-1 Fc Chimera Protein, CF

Catalog #: 5988-FZ
2 Citations Datasheet / COA / SDS

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5988-FZ-050

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Citations (2)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Frizzled-1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-1/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-1/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 20-80 ng/mL.
Optimal concentrations should be determined by each laboratory for each application.

Source

Mouse myeloma cell line, NS0-derived human Frizzled-1 protein

Human Frizzled-1 (Gln73 - His253) Accession # Q9UP38	IEGRD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Accession #

Q9UP38

N-terminal Sequence
Analysis

Gly82 & No results obtained: Gln73 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

46.2 kDa (monomer)

SDS-PAGE

59-66 kDa, reducing conditions

Product Datasheets

5988-FZ

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5988-FZ

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 300 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Frizzled-1

Frizzled-1 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors (1 - 3). Frizzled proteins are thought to be G-protein-coupled (1, 4). Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance (1). Frizzled-1 can also act as a Wnt antagonist, for example, when sequestered from LRP-5/6 by LRP-1 (5, 6). Human Frizzled-1 cDNA encodes 647 aa including a 69 aa signal peptide, an extracellular cysteine-rich domain (CRD, aa 111 - 230) that binds Wnts and is highly conserved among Frizzleds, a linker region, the 7TM region, and the C-terminal cytoplasmic domain (2). Most Frizzleds, including Frizzled-1, contain a C-terminal PDZ binding motif (1). Within aa 73 - 253, human Frizzled-1 shares 94%, 93%, 97% and 98% aa identity with mouse, rat, bovine and canine Frizzled-1, respectively. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD (1, 7). Frizzled-1 shares high aa identity (~76%) with Frizzled-2 and -7, but may differ in expression and Wnt selectivity (3, 8). Specifically, Wnt-3a interaction with rat Frizzled-1, but not Frizzled-2, blocks toxicity of the Alzheimers A beta peptide on PC12 cells (8). In bone, Frizzled-1 may be part of a feedback mechanism to modulate the effects of BMP-2 on mesenchymal cells (6, 9). Frizzled-1 is also one of the purported Wnt10b receptors whose signaling inhibits adipocyte differentiation (10). It is widely expressed, with mRNA detection in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung and kidney (2).

References

Schulte, G. and B. Vitezslav (2007) Trends Pharmacol. Sci. 28:518.
Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
Gazit, A. et al. (1999) Oncogene 18:5959.
Liu, T. et al. (1999) J. Biol. Chem. 274:33539.
Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.
Kaykas, A. et al. (2004) Nat. Cell Biol. 6:52.
Chacon, M.A. et al. (2008) J. Cell. Physiol. 217:215.
Yerges, L.M. et al. (2009) J. Bone Miner. Res. 24:87.
Bennett, C.N. et al. (2002) J. Biol. Chem. 277:30998.

Entrez Gene IDs

8321 (Human); 14362 (Mouse)

Alternate Names

DKFZp564G072; FLJ95923; frizzled family receptor 1; frizzled homolog 1; frizzled, Drosophila, homolog of, 1; Frizzled1; Frizzled-1; fz-1; FZD1; fzE1; hFz1; seven transmembrane spanning receptor; Wnt receptor

Related Research Areas

Citations for Recombinant Human Frizzled-1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy
Authors: R Chidiac, M Abedin, G Macleod, A Yang, PE Thibeault, LL Blazer, JJ Adams, L Zhang, H Roehrich, HN Jo, S Seshagiri, SS Sidhu, HJ Junge, S Angers
Embo Molecular Medicine, 2021-06-09;0(0):e13977.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay