Recombinant Human Frizzled-8 Fc Chimera Protein, CF

Catalog #: 6129-FZ
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
6129-FZ-050

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Citations (4)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Frizzled-8 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 15-75 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN) Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated recombinant mouse (rm) Wnt-3a or rmWnt-5a at 50-100 ng/mL and Recombinant Human (rh) Frizzled-8 Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-8 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 7-28 ng/mL.
Optimal concentrations should be determined by each laboratory for each application.

Source

Chinese Hamster Ovary cell line, CHO-derived human Frizzled-8 protein

Human Frizzled-8 (Ala25-Pro172) Accession # Q9H461	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9H461

N-terminal Sequence
Analysis

Ala25 & Ala28

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

43.3 kDa (monomer)

SDS-PAGE

55-65 kDa, reducing conditions

Product Datasheets

6129-FZ

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6129-FZ

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: Frizzled-8

Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors (1-2). Frizzled proteins are thought to be G-protein-coupled (1). Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance (1). Human Frizzled-8 cDNA encodes 694 amino acids (aa) including a 27 aa signal peptide, a 247 aa extracellular domain (ECD), the 7TM region (aa 276-605), and a C-terminal cytoplasmic domain with a PDZ binding motif (aa 606-694) (2). The ECD includes a cysteine-rich region (CRD, aa 30-151) that binds Wnts and is highly conserved among Frizzleds, and a linker region. Within aa 28-172, human Frizzled-8 shares 99%, 99%, 90% and 85% aa identity with mouse, rat, Xenopus and zebrafish Frizzled-8, respectively. It also shares 82% aa identity with human Frizzled-5. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD (1, 3). During mouse development, Frizzled-8 is expressed in tissues that are important for organizing the anterior-posterior axis (4). Interactions of Frizzled-8 with several non-Wnt ligands have been identified. Interaction with Frizzled-8 and LRP-6 allows R-spondins to activate beta -catenin signaling pathways, while interaction with IGFBP-4 (insulin-like growth factor binding protein (4) or CTGF (connective tissue growth factor) inhibits Wnt signaling (5-7). These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas (5-10).

References

Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
Saitoh, T. et al. (2001) Int. J. Oncol. 18:991.
Dann, C.E. et al. (2001) Nature 412:86.
Lu C.C. et al. (2004) Gene Expr. Patterns 4:569.
Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
Zhu, W. et al. (2008) Nature 454:345.
Mercurio, S. et al. (2004) Development 131:2137.
Chesnutt, C. et al. (2004) Dev. Biol. 274:334.
Yamashita, J.K. et al. (2005) FASEB J. 19:1534.
DeAlmeida, V.I. et al. (2007) Cancer Res. 67:5371.

Entrez Gene IDs

8325 (Human); 14370 (Mouse)

Alternate Names

frizzled (Drosophila) homolog 8; frizzled 8; frizzled family receptor 8; frizzled homolog 8 (Drosophila); Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8

Related Research Areas

Citations for Recombinant Human Frizzled-8 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy
Authors: R Chidiac, M Abedin, G Macleod, A Yang, PE Thibeault, LL Blazer, JJ Adams, L Zhang, H Roehrich, HN Jo, S Seshagiri, SS Sidhu, HJ Junge, S Angers
Embo Molecular Medicine, 2021-06-09;0(0):e13977.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway
Authors: K Saito-Diaz, H Benchabane, A Tiwari, A Tian, B Li, JJ Thompson, AS Hyde, LM Sawyer, JN Jodoin, E Santos, LA Lee, RJ Coffey, RD Beauchamp, CS Williams, AK Kenworthy, DJ Robbins, Y Ahmed, E Lee
Dev. Cell, 2018-03-12;44(5):566-581.e8.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Novel Bi-specific Domain Antibody to LRP6 Inhibits Wnt and R-spondin Ligand-induced Wnt Signaling and Tumor Growth
Mol Cancer Res, 2016-07-11;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay