Recombinant Human FUBI/MNSF beta Protein, CF

Recombinant Human FUBI/MNSF beta Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: FUBI/MNSF beta

The ubiquitin-like protein FUBI/ MNSF beta (Monoclonal Nonspecific Suppressor Factor Beta) is encoded by the FAU gene, and is translated as a pro-form consisting of FUBI fused to the ribosomal S30 protein. S30 is cleaved in a post-translational reaction, releasing the mature 74 amino acid FUBI/MNSF beta protein. FUBI has a C-terminal gly-gly motif common to many ubiquitin-like proteins.

FUBI/ MNSF beta conjugation to Bcl-G has been shown to regulate the ERK1/2-MAPK cascade in macrophage cell lines, and may be implicated in TLR4-mediated signal transduction. Conjugation to endophilin II regulates dectin-1-mediated phagocytosis and inflammatory responses, and may be implicated in TLR2 signaling pathway.

Related Research Areas

• Ubiquitin-like Modifiers (UBLs) and Regulators