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Recombinant Human G-CSF Protein

Catalog #: 214-CS
56 Citations 2 Reviews Datasheet / COA / SDS

Carrier Free

Catalog # Availability Size / Price Qty
214-CS-005/CF
214-CS-025/CF

With Carrier

Catalog # Availability Size / Price Qty
214-CS-005
214-CS-025
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Recombinant Human G-CSF Protein Bioactivity
2 Images
Product Details
Citations (56)
FAQs
Supplemental Products
Reviews (2)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human G-CSF Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116. The ED50 for this effect is 10-60 pg/mL.
Source
E. coli-derived human G-CSF protein
Thr31-Pro204, with an N-terminal Met
Accession #
NP_757373
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
18.8 kDa
SDS-PAGE
17-19 kDa, reducing conditions

Product Datasheets

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214-CS (with carrier)

Product Datasheet
COA
SDS

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214-CS/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

214-CS

Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid with BSA as a carrier protein.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

214-CS/CF

Formulation Supplied as a 0.2 μm filtered solution in Acetic Acid.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Scientific Data

Bioactivity Recombinant Human G-CSF Protein Bioactivity View Larger

Recombinant Human G-CSF (Catalog # 214-CS) stimulates cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50 for this effect is 10-60 pg/mL.

SDS-PAGE Recombinant Human G-CSF Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human G-CSF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18.5 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

Long Name
Granulocyte Colony Stimulating Factor
Entrez Gene IDs
1440 (Human); 12985 (Mouse)
Alternate Names
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; Pluripoietin

Citations for Recombinant Human G-CSF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

56 Citations: Showing 1 - 10
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  1. Retrotransposons in Werner syndrome-derived macrophages trigger type I interferon-dependent inflammation in an atherosclerosis model
    Authors: Paul, SK;Oshima, M;Patil, A;Sone, M;Kato, H;Maezawa, Y;Kaneko, H;Fukuyo, M;Rahmutulla, B;Ouchi, Y;Tsujimura, K;Nakanishi, M;Kaneda, A;Iwama, A;Yokote, K;Eto, K;Takayama, N;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. SARS-CoV-2 superinfection in CD14+ monocytes with latent human cytomegalovirus (HCMV) promotes inflammatory cascade
    Authors: Payen, SH;Adhikari, K;Petereit, J;Uppal, T;Rossetto, CC;Verma, SC;
    Virus research
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Encapsulated Streptococcus suis impairs optimal neutrophil functions which are not rescued by priming with colony-stimulating factors
    Authors: Bleuzé, M;Lavoie, JP;Bédard, C;Gottschalk, M;Segura, M;
    PloS one
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  4. c-Met Mediated Cytokine Network Promotes Brain Metastasis of Breast Cancer by Remodeling Neutrophil Activities
    Authors: Liu, Y;Smith, MR;Wang, Y;D'Agostino, R;Ruiz, J;Lycan, T;Kucera, GL;Miller, LD;Li, W;Chan, MD;Farris, M;Su, J;Song, Q;Zhao, D;Chandrasekaran, A;Xing, F;
    Cancers
  5. Hydrogel-Based Pre-Clinical Evaluation of Repurposed FDA-Approved Drugs for AML
    Authors: JR James, J Curd, JC Ashworth, M Abuhantash, M Grundy, CH Seedhouse, KP Arkill, AJ Wright, CLR Merry, A Thompson
    International Journal of Molecular Sciences, 2023-02-20;24(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Human JAK1 gain of function causes dysregulated myelopoeisis and severe allergic inflammation
    Authors: CM Biggs, A Cordeiro-S, SV Prykhozhij, AP Deveau, Y Lin, KL Del Bel, F Orben, RJ Ragotte, A Saferali, S Mostafavi, L Dinh, D Dai, KG Weinacht, K Dobbs, L Ott de Bru, M Sharma, K Tsai, JJ Priatel, RA Schreiber, J Rozmus, MC Hosking, KE Shopsowitz, ML McKinnon, S Vercautere, M Seear, LD Notarangel, FC Lynn, JN Berman, SE Turvey
    JCI Insight, 2022-12-22;7(24):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  7. Generation of the human pluripotent stem cell lines KUMi005-A from a patients with multiple myeloma
    Authors: JH Kim, JH Hong, KW Kang, BH Lee, Y Park, BS Kim
    Stem Cell Research, 2022-10-13;65(0):102939.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells
    Authors: YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra
    Science Advances, 2022-08-24;8(34):eabn5522.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Generation of human induced iPSC KUMi003-A from acute promyelocytic leukemia (APL) M3
    Authors: JH Kim, KW Kang, BH Lee, Y Park, BS Kim
    Oncogene, 2022-07-09;63(0):102861.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Design of a US28 ORF Deletion Virus in a Temperature-Sensitive Cytomegalovirus Strain Fails to Promote Lytic Replication in Hematopoietic Cells
    Authors: BA Krishna, AB Wass, EA Murphy, CM O'Connor
    Viruses, 2022-06-12;14(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Generation of a human induced pluripotent stem cell line KUMi006 from a patient with multiple myeloma
    Authors: JH Hong, JH Kim, KW Kang, BH Lee, Y Park, BS Kim
    Stem Cell Research, 2022-03-28;61(0):102767.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. A human pluripotent stem cell line KUMi004-A generated from a patient with chronic lymphocytic leukemia
    Authors: JH Kim, KW Kang, BH Lee, Y Park, H Jung Sung, BS Kim
    Stem Cell Research, 2022-01-15;60(0):102668.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Generation of induced pluripotent stem cell line KUMi002-A with normal karyotype from a patient with Philadelphia chromosome-positive chronic myeloid leukemia
    Authors: JH Kim, KW Kang, BH Lee, Y Park, BS Kim
    Stem Cell Research, 2021-07-12;55(0):102465.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. A digital single-molecule nanopillar SERS platform for predicting and monitoring immune toxicities in immunotherapy
    Authors: J Li, A Wuethrich, AAI Sina, HH Cheng, Y Wang, A Behren, PN Mainwaring, M Trau
    Nature Communications, 2021-02-17;12(1):1087.
    Species: Human
    Sample Types: Reference Standard
  15. Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocation
    Authors: SJ Lee, JH Kim, KW Kang, BH Lee, BS Kim
    Stem Cell Res, 2020-10-15;49(0):102030.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  16. Activator protein-1 transactivation of the major immediate early locus is a determinant of cytomegalovirus reactivation from latency
    Authors: BA Krishna, AB Wass, CM O'Connor
    Proc. Natl. Acad. Sci. U.S.A., 2020-08-11;117(34):20860-20867.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Supporting data on enhanced reprogramming of human CD34+ hematopoietic stem cells to induced pluripotent stem cells using human placenta-derived cell conditioned medium
    Authors: SJ Lee, JH Kim, KW Kang, Y Park, BS Kim
    Data Brief, 2020-08-06;32(0):106140.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Generation of an induced pluripotent stem cell line KUMCi001-A from CD34+ bone marrow cells of a patient with acute lymphoblastic leukemia using human placenta-derived cell conditioned medium
    Authors: SJ Lee, KW Kang, JH Kim, Y Park, BS Kim
    Stem Cell Res, 2020-07-13;47(0):101913.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  19. Etoposide-mediated interleukin-8 secretion from bone marrow stromal cells induces hematopoietic stem cell mobilization
    Authors: KW Kang, SJ Lee, JH Kim, BH Lee, SJ Kim, Y Park, BS Kim
    BMC Cancer, 2020-07-02;20(1):619.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Targeting macrophage checkpoint inhibitor SIRPa for anticancer therapy
    Authors: J Liu, S Xavy, S Mihardja, S Chen, K Sompalli, D Feng, TS Choi, B Agoram, R Majeti, IL Weissman, JP Volkmer
    JCI Insight, 2020-06-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Generation of Hematopoietic-Like Stem Cells from Adult Human Peripheral Blood Following Treatment with Platelet-Derived Mitochondria
    Authors: H Yu, W Hu, X Song, D Descalzi-M, Z Yang, R Korngold, Y Zhao
    Int J Mol Sci, 2020-06-15;21(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Detection of subclonal SETBP1 and JAK3 mutations in juvenile myelomonocytic leukemia using droplet digital PCR
    Authors: M Wakamatsu, Y Okuno, N Murakami, S Miwata, H Kitazawa, K Narita, S Kataoka, D Ichikawa, M Hamada, R Taniguchi, K Suzuki, N Kawashima, E Nishikawa, A Narita, N Nishio, S Kojima, H Muramatsu, Y Takahashi
    Leukemia, 2020-04-20;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. CRISPR/Cas9 mediated ELANE knockout enables neutrophilic maturation of primary hematopoietic stem and progenitor cells and induced pluripotent stem cells of severe congenital neutropenia patients
    Authors: M Nasri, M Ritter, P Mir, B Dannenmann, N Aghaallaei, D Amend, V Makaryan, Y Xu, B Fletcher, R Bernhard, I Steiert, K Hahnel, J Berger, I Koch, B Sailer, K Hipp, C Zeidler, M Klimiankou, B Bajoghli, DC Dale, K Welte, J Skokowa
    Haematologica, 2019-06-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector
    Authors: T Okumura, Y Horie, CY Lai, HT Lin, H Shoda, B Natsumoto, K Fujio, E Kumaki, T Okano, S Ono, K Tanita, T Morio, H Kanegane, H Hasegawa, F Mizoguchi, K Kawahata, H Kohsaka, H Moritake, H Nunoi, H Waki, SI Tamaru, T Sasako, T Yamauchi, T Kadowaki, H Tanaka, S Kitanaka, K Nishimura, M Ohtaka, M Nakanishi, M Otsu
    Stem Cell Res Ther, 2019-06-24;10(1):185.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Therapeutic discovery for marrow failure with MDS predisposition using pluripotent stem cells
    Authors: M Ruiz-Gutie, ÖV Bölükba??, G Alexe, AG Kotini, K Ballotti, CE Joyce, DW Russell, K Stegmaier, K Myers, CD Novina, EP Papapetrou, A Shimamura
    JCI Insight, 2019-04-30;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Differentiation, Differentiation
  26. Molecular transitions in early progenitors during human cord blood hematopoiesis
    Authors: S Zheng, E Papalexi, A Butler, W Stephenson, R Satija
    Mol. Syst. Biol., 2018-03-15;14(3):e8041.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own Demise
    Authors: H Zhao, S Gulesseria, MC Malinao, S Kumar-Gane, J Song, MS Chang, MM Williams, Z Zeng, M Mattie, BA Mendelsohn, DR Stover, F Doñate
    Mol. Cancer Ther., 2017-05-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. G-CSF Receptor Blockade Ameliorates Arthritic Pain and Disease
    Authors: MC Lee, JA McCubbin, AD Christense, DP Poole, P Rajasekhar, T Lieu, NW Bunnett, S Garcia-Car, A Erickson, SM Brierley, R Saleh, A Achuthan, AJ Fleetwood, RL Anderson, JA Hamilton, AD Cook
    J. Immunol, 2017-03-20;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  29. Differentiation of induced pluripotent stem cell-derived neutrophil granulocytes from common marmoset monkey (Callithrix jacchus)
    Authors: Thomas Müller
    Transfusion, 2016-11-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Improved hematopoietic differentiation of human pluripotent stem cells via estrogen receptor signaling pathway
    Cell Biosci, 2016-08-30;6(1):50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Cancer stem cell-secreted macrophage migration inhibitory factor stimulates myeloid derived suppressor cell function and facilitates glioblastoma immune evasion
    Stem Cells, 2016-05-27;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis.
    Authors: Wang F, Gao J, Malisani A, Xi X, Han W, Wan X
    BMC Biotechnol, 2015-11-16;15(0):105.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Preserved in vivo reconstitution ability of PBSCs cryopreserved for a decade at -80 degrees C.
    Authors: Shima T, Iwasaki H, Yamauchi T, Kadowaki M, Kiyosuke M, Mochimaru T, Takenaka K, Miyamoto T, Akashi K, Teshima T
    Bone Marrow Transplant, 2015-06-22;50(9):1195-200.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Functional Characterization of D9, a Novel Deazaneplanocin A (DZNep) Analog, in Targeting Acute Myeloid Leukemia (AML).
    Authors: Jiang X, Lim C, Li Z, Lee P, Yatim S, Guan P, Li J, Zhou J, Pan J, Chng W, Chai C, Yu Q
    PLoS ONE, 2015-04-30;10(4):e0122983.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Reversible lineage-specific priming of human embryonic stem cells can be exploited to optimize the yield of differentiated cells.
    Authors: Lee J, Graham M, Collins T, Lee J, Hong S, McNicol A, Shapovalova Z, Bhatia M
    Stem Cells, 2015-04-01;33(4):1142-52.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Transcriptome analysis of Kaposi's sarcoma-associated herpesvirus during de novo primary infection of human B and endothelial cells.
    Authors: Purushothaman P, Thakker S, Verma S
    J Virol, 2014-12-31;89(6):3093-111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Targeted 25-hydroxyvitamin D3 1alpha-hydroxylase adoptive gene therapy ameliorates dss-induced colitis without causing hypercalcemia in mice.
    Authors: Li B, Baylink D, Walter M, Lau K, Meng X, Wang J, Cherkas A, Tang X, Qin X
    Mol Ther, 2014-10-20;23(2):339-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Granulocyte-Colony Stimulating Factor related pathways tested on an endometrial ex-vivo model.
    Authors: Rahmati M, Petitbarat M, Dubanchet S, Bensussan A, Chaouat G, Ledee N
    PLoS ONE, 2014-10-02;9(9):e102286.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  39. Cancer-associated adipose tissue promotes breast cancer progression by paracrine oncostatin M and Jak/STAT3 signaling.
    Authors: Lapeire L, Hendrix A, Lambein K, Van Bockstal M, Braems G, Van Den Broecke R, Limame R, Mestdagh P, Vandesompele J, Vanhove C, Maynard D, Lehuede C, Muller C, Valet P, Gespach C, Bracke M, Cocquyt V, Denys H, De Wever O
    Cancer Res, 2014-09-24;74(23):6806-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Antagonistic determinants controlling replicative and latent states of human cytomegalovirus infection.
    Authors: Umashankar, Mahadeva, Rak, Michael, Bughio, Farah, Zagallo, Patricia, Caviness, Katie, Goodrum, Felicia
    J Virol, 2014-03-12;88(11):5987-6002.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.
    Authors: Klimenkova O, Ellerbeck W, Klimiankou M, Unalan M, Kandabarau S, Gigina A, Hussein K, Zeidler C, Welte K, Skokowa J
    Blood, 2013-12-18;123(8):1239-49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Patient-specific naturally gene-reverted induced pluripotent stem cells in recessive dystrophic epidermolysis bullosa.
    Authors: Tolar J, McGrath J, Xia L, Riddle M, Lees C, Eide C, Keene D, Liu L, Osborn M, Lund T, Blazar B, Wagner J
    J Invest Dermatol, 2013-12-06;134(5):1246-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. G-protein-coupled receptor agonist BV8/prokineticin-2 and STAT3 protein form a feed-forward loop in both normal and malignant myeloid cells.
    Authors: Xin, Hong, Lu, Rongze, Lee, Heehyoun, Zhang, Wang, Zhang, Chunyan, Deng, Jiehui, Liu, Yong, Shen, Shudan, Wagner, Kay-Uwe, Forman, Stephen, Jove, Richard, Yu, Hua
    J Biol Chem, 2013-04-02;288(19):13842-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. The canonical Wnt pathway shapes niches supportive of hematopoietic stem/progenitor cells.
    Authors: Ichii, Michiko, Frank, Mark Bar, Iozzo, Renato V, Kincade, Paul W
    Blood, 2011-11-23;119(7):1683-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. ETV6-RUNX1 promotes survival of early B lineage progenitor cells via a dysregulated erythropoietin receptor.
    Authors: Torrano V, Procter J, Cardus P
    Blood, 2011-09-07;118(18):4910-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  46. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.
    Authors: Niwa A, Heike T, Umeda K
    PLoS ONE, 2011-07-27;6(7):e22261.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. Hematopoietic differentiation of induced pluripotent stem cells from patients with mucopolysaccharidosis type I (Hurler syndrome).
    Authors: Tolar J, Park I, Xia L, Lees C, Peacock B, Webber B, McElmurry R, Eide C, Orchard P, Kyba M, Osborn M, Lund T, Wagner J, Daley G, Blazar B
    Blood, 2010-10-29;117(3):839-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  48. Does granulocyte colony-stimulating factor ameliorate the proinflammatory response in human meningococcal septic shock?
    Authors: Rojahn A, Brusletto B, Ovstebo R, Haug KB, Kierulf P, Brandtzaeg P
    Crit. Care Med., 2008-09-01;36(9):2583-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  49. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008-05-27;36(8):1035-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. Unique structural determinants for Stat3 recruitment and activation by the granulocyte colony-stimulating factor receptor at phosphotyrosine ligands 704 and 744.
    Authors: Shao H, Xu X, Jing N, Tweardy DJ
    J. Immunol., 2006-03-01;176(5):2933-41.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
    Authors: Ramachandran R, Morice AH, Compton SJ
    Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):133-41.
    Applications: ELISA (Standard)
  52. Proinflammatory mediators elicit secretion of the intracellular B-lymphocyte stimulator pool (BLyS) that is stored in activated neutrophils: implications for inflammatory diseases.
    Authors: Scapini P, Carletto A, Nardelli B, Calzetti F, Roschke V, Merigo F, Tamassia N, Pieropan S, Biasi D, Sbarbati A, Sozzani S, Bambara L, Cassatella MA
    Blood, 2004-09-09;105(2):830-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  53. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004-08-01;21(2):241-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  54. Enhanced circulating half-life and hematopoietic properties of a human granulocyte colony-stimulating factor/immunoglobulin fusion protein.
    Authors: Cox GN, Smith DJ, Carlson SJ, Bendele AM, Chlipala EA, Doherty DH
    Exp. Hematol., 2004-05-01;32(5):441-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  55. High-affinity autoantibodies specifically eliminate granulocyte-macrophage colony-stimulating factor activity in the lungs of patients with idiopathic pulmonary alveolar proteinosis.
    Authors: Uchida K, Nakata K, Trapnell BC, Terakawa T, Hamano E, Mikami A, Matsushita I, Seymour JF, Oh-Eda M, Ishige I, Eishi Y, Kitamura T, Yamada Y, Hanaoka K, Keicho N
    Blood, 2003-09-25;103(3):1089-98.
    Species: Human
    Sample Types: Serum
    Applications: SDS-Page
  56. Proliferating dendritic cell progenitors in human blood.
    Authors: Romani N, Gruner S, Brang D, Kampgen E, Lenz A, Trockenbacher B, Konwalinka G, Fritsch P, Steinman R, Schuler G
    J Exp Med, 1994-07-01;180(1):83-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human G-CSF Protein
By Meilyn Sylvestre on 11/20/2020
Application: Stem/Immune cell maintenance or differentiation
Recombinant Human G-CSF Protein
By Anonymous on 11/06/2018
Application: Immunoassay Standard

The recombinant G-CSF was used as a calibrator in an ELISA with sensitivity at 18pg/ml. The RnD Systems G-CSF antibody MAB214 was used as the capture in this assay along with BAF214 as the detection.

Immunoassay Standard Recombinant Human G-CSF Protein 214-CS