Recombinant Human Galectin-2 (HEK-293-expressed) Protein, CF

Catalog #: 9874-GA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9874-GA-050

Recombinant Human Galectin-2 (HEK-293-expressed) Protein Bioactivity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Galectin-2 (HEK-293-expressed) Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED₅₀for this effect is 5-25 μg/mL. Measured by the ability of the immobilized protein to enhance the adhesion of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 1-6 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human Galectin-2 protein
Met1-Glu132

Accession #

P05162

N-terminal Sequence
Analysis

Thr2

Predicted Molecular Mass

15 kDa

SDS-PAGE

12-15 kDa, reducing conditions

Product Datasheets

9874-GA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9874-GA

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose.
Reconstitution	Reconstitute at 400 μg/mL in water.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human Galectin-2 (Catalog # 9874-GA) agglutinates human red blood cells. The ED₅₀ for this effect is 5-25 μg/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human Galectin‑2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 12-15 kDa.

Reconstitution Calculator

Background: Galectin-2

Galectins constitute a large family of carbohydrate-binding proteins with specificity for N‑acetyl-lactosamine-containing glycoproteins. To date, 15 mammalian galectins, which share structural similarities in their carbohydrate-recognition domains (CRD), have been identified. Twelve galectin genes are found in humans, including two for galectin-9. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14, -15), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectin (galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. (1). Galectin-2 is an approximately 14-kDa homodimeric protein, and like other prototype galectins, consists of a single CRD (2-4). Human Galectin-2 shares 66% and 67% amino acid sequence identity with mouse and rat Galectin-2, respectively. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (5-7). Galectin-2 is expressed in hepatoma, stomach epithelial cells and in colorectal and neural tumors. The specific functions of Galectin-2 have not been reported but increased serum levels of Galectin-2 have been associated with metastatic cancer and this may also be involved in cancer cell adhesion to vascular endothelium (8).

References

Cummings, R.D. and Liu, F. (2009) Essentials of Glycobiology. 2nd edition. Chapter 33.
Hokama, A. et al. (2008) World J. Gastroenterol. 14:5133.
Barondes, S.H. et al. (1994) Cell 76:597.
Hirabayashi, J. and K. Kasai (1993) Glycobiology 3:297.
Rabinovich, A. et al. (2002) Trends in Immunol. 23:313.
Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
Hughes, R.C. (2001) Biochimie 83:667.
Barrow, H. et al. (2011) Clinical Cancer Research 22:7035.

Entrez Gene IDs

3957 (Human); 107753 (Mouse); 171134 (Rat)

Alternate Names

Beta-galactoside-binding lectin L-14-II; GAL2; Gal-2; galectin 2; Galectin2; Galectin-2; HL14gal-2; Lactose-binding lectin 2; lectin, galactoside-binding, soluble, 2; LGALS2; MGC75071; S-Lac lectin 2