Recombinant Human GITR/TNFRSF18 Fc Chimera Protein, CF

Catalog #: 689-GR Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
689-GR-100

R&D Systems Recombinant Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human GITR/TNFRSF18 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human (rh) GITR/TNFRSF18 Fc Chimera at 2 µg/mL (100 µL/well) can bind rhGITR Ligand with a linear range of 0.3-20 ng/mL.

Source

Mouse myeloma cell line, NS0-derived human GITR/TNFRSF18 protein

Human GITR (Gln26-Glu161) (Thr45Ala) Accession # Q9Y5U5	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9Y5U5

N-terminal Sequence
Analysis

No results obtained: Gln26 predicted

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

41 kDa (monomer)

SDS-PAGE

50 kDa, reducing conditions

Product Datasheets

689-GR

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

689-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: GITR/TNFRSF18

GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation (1, 2). Mature human GITR consists of a 137 amino acid (aa) extracellular domain (ECD) with three tandem TNFR cysteine-rich repeats, a 21 aa transmembrane segment, and a 58 aa cytoplasmic domain (3, 4). Within the ECD, human GITR shares 55% and 60% aa sequence identity with mouse and rat GITR, respectively. Alternative splicing generates an isoform with a short deletion in the cytoplasmic domain and a potentially secreted isoform that is substituted within the third TNFR repeat and lacks the transmembrane and cytoplasmic regions. GITR is expressed on CD4⁺CD25⁺ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes (4-6), and it is upregulated on antigen-activated conventional CD4⁺ and CD8⁺ T cells (3, 4, 6, 7). GITR binding by GITR Ligand/TNFSF18 costimulates the proliferation and activation of CD4⁺ or CD8⁺ conventional T cells (3, 7-9). It also induces the proliferation of Treg (8, 10) but inhibits the ability of Treg to suppress immune responses (5, 8, 11-13). This can result in the development of autoimmunity, increased tumor cell killing by effector T cells (5, 11), and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease (10, 14). GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching (15).

References

Clouthier, D.L. and T.H. Watts (2014) Cytokine Growth Factor Rev. 25:91.
Ronchetti, S. et al. (2015) J. Immunol. Res. 2015:171520.
Gurney, A.L. et al. (1999) Curr. Biol. 9:215.
Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
Shimizu, J. et al. (2002) Nat. Immunol. 3:135.
Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
Muriglan, S.J. et al. (2004) J. Exp. Med. 200:149.
Ronchetti, S. et al. (2004) Eur. J. Immunol. 34:613.
Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
Ephrem, A. et al. (2013) Eur. J. Immunol. 43:2421.
Ko, K. et al. (2005) J. Exp. Med. 202:885.
Ji, H. et al. (2004) J. Immunol. 172:5823.
Stephens, G.L. et al. (2004) J. Immunol. 173:5008.
Patel, M. et al. (2005) Eur. J. Immunol. 35:3581.
O'Keeffe, G.W. et al. (2008) Nat. Neurosci. 11:135.

Long Name

Glucocorticoid Induced TNF Receptor Family Related Gene

Entrez Gene IDs

8784 (Human); 21936 (Mouse); 500598 (Rat); 102146362 (Cynomolgus Monkey)

Alternate Names

Activation-inducible TNFR family receptor; AITR; AITRTNF receptor superfamily activation-inducible protein; CD357 antigen; CD357; GITR; GITR-D; GITRtumor necrosis factor receptor superfamily member 18; Glucocorticoid-induced TNFR-related protein; TNFRSF18; tumor necrosis factor receptor superfamily, member 18