Recombinant Human GPR37 Protein, CF

Catalog #: 9609-GP Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9609-GP-050

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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Recombinant Human GPR37 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Bioassay data are not available.

Source

Chinese Hamster Ovary cell line, CHO-derived human GPR37 protein
Leu28-Met265, with a C-terminal 6-His tag

Accession #

O15354

N-terminal Sequence
Analysis

Leu28

Predicted Molecular Mass

26 kDa

SDS-PAGE

37-53 kDa, reducing conditions

Product Datasheets

9609-GP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9609-GP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: GPR37

GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor) is a 587 aa, 7-transmembrane receptor for the neuroprotective and glioprotective factor prosaposin (1, 4). It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus (2). It is a substrate of the E3 ubiquitin ligase, parkin, which is up-regulated during endoplasmic reticulum stress (3). In a juvenile form of Parkinson's disease, GPR37 accumulates, contributing to stress-induced neuronal cell death (2). The extracelluar domains (aa 27-265) of human and mouse GPR37 share 68% aa identity.

References

Marazziti, D. et al. (1997) Genomics, 45:68.
Donohue, P.J. et al. (1998) Brain Res Mol Brain Res. 54:152.
Omura, T. et al. (2006) J Neurochem. 99:1456.
Meyer, R.C. et al. (2013) Proc. Natl. Acad. Sci. U. S. A. 110: 9529

Long Name

G Protein-coupled Receptor 37

Entrez Gene IDs

2861 (Human); 14763 (Mouse); 117549 (Rat)

Alternate Names

EDNRBL; Endothelin B receptor-like protein 1; ETBR-LP-1; G protein-coupled receptor 37 (endothelin receptor type B-like); GPR37; hET(B)R-LP; PAELR; PAELRETBR-LP-1; Parkin-associated endothelin receptor-like receptor; probable G-protein coupled receptor 37