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Recombinant Human GST-UbcH5a/UBE2D1 Protein, CF

Catalog #: E2-615
3 Citations Datasheet / COA / SDS

Discontinued Product

E2-615 has been discontinued.
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human GST-UbcH5a/UBE2D1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human GST-UbcH5a/UBE2D1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UbcH5a/UBE2D1 concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH5a/UBE2D1 protein
Contains an N-terminal GST (glutathione S-transferase) tag
Accession #
P51668
Predicted Molecular Mass
44 kDa

Product Datasheets

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E2-615

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-615

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UbcH5a/UBE2D1

Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT) (1,2). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively (3). Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins (4). UbcH5a/UBE2D1 interacts with the E3, E6-AP, to conjugate Ubiquitin to the tumor suppressor, p53 (1). Additional protein targets of UbcH5a/UBE2D1 include c-Fos, RIP1, and HIF-1 (5,6). Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses (7).

 

References
  1. Scheffner, M. et al. (1994) Proc. Natl. Acad. Sci. USA 91:8797.
  2. Gehrke, S. et al. (2003) Blood 101:3288.
  3. Jensen, J. et al. (1995) J. Biol. Chem. 270:30408.
  4. Lorick, K. et al. (2005) Methods Enzymol. 398:54.
  5. Stancovski, I. et al. (1995) Mol. Cell Biol. 15:7106.
  6. Dynek, J. et al. (2010) EMBO J. 29:4128.
  7. Vanni, E. et al. (2012) J. Virol. 86:6323.
Entrez Gene IDs
7321 (Human); 216080 (Mouse); 361831 (Rat)
Alternate Names
E2(17)KB1; Stimulator of Fe transportUBC4/5 homolog; UBC4/5; UBC5A; UbcH5; UbcH5a; UBCH5AEC 6.3.2.19; UBCH5SFT; UBE2D1; Ubiquitin carrier protein D1; ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2-17 kDa 1; ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D1

Citations for Recombinant Human GST-UbcH5a/UBE2D1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. A role for LSH in facilitating DNA methylation by DNMT1 through enhancing UHRF1 chromatin association
    Authors: M Han, J Li, Y Cao, Y Huang, W Li, H Zhu, Q Zhao, JJ Han, Q Wu, J Li, J Feng, J Wong
    Nucleic Acids Res, 2020-12-02;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. Coordinated regulation of XPA stability by ATR and HERC2 during nucleotide excision repair.
    Authors: Lee, T-H, Park, J-M, Leem, S-H, Kang, T-H
    Oncogene, 2012-11-26;33(1):19-25.
    Applications: Bioassay
  3. The H3K4me3 histone demethylase Fbxl10 is a regulator of chemokine expression, cellular morphology, and the metabolome of fibroblasts.
    Authors: Janzer, Andreas, Stamm, Katrin, Becker, Astrid, Zimmer, Andreas, Buettner, Reinhard, Kirfel, Jutta
    J Biol Chem, 2012-07-23;287(37):30984-92.
    Applications: Bioassay

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