Recombinant Human GST-UbcH8/UBE2L6 Protein, CF

Name: Recombinant Human GST-UbcH8/UBE2L6 Protein, CF
Brand: R&D Systems
SKU: E2-643
Price: 272 USD

Catalog #: E2-643 Datasheet / COA / SDS

Discontinued Product

E2-643 has been discontinued.

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Recombinant Human GST-UbcH8/UBE2L6 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Recombinant Human GST-UbcH8/UBE2L6 is a member of the Ub AND ISG15-conjugating (E2) enzyme family that receives Ub AND ISG15 from a Ub AND ISG15-activating (E1) enzyme and subsequently interacts with a Ub AND ISG15 ligase (E3) to conjugate Ub AND ISG15 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UbcH8/UBE2L6 concentration of 0.1-1 μM.

Source

E. coli-derived human UbcH8 protein
Contains an N-terminal GST (glutathione S-transferase) tag

Accession #

O14933

Predicted Molecular Mass

44 kDa

Product Datasheets

E2-643

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-643

Formulation	X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: UbcH8

Ubiquitin-conjugating Enzyme H8 (UbcH8), also known as Ubiquitin-conjugating Enzyme E2L 6 (UBE2L6), has a predicted molecular weight of 18 kDa. Human UbcH8 shares 76% and 74% amino acid sequence identity with mouse and rat UBE2L6, respectively. UbcH8 is capable of transferring either Ubiquitin or the Ubiquitin-like protein ISG15 Ubiquitin-like Modifier (ISG15) to target proteins (1,2). ISG15 conjugation is mediated by UbcH8 following IFN-alpha or IFN-beta stimulation (3,4). UbcH8 interacts with a variety of Ubiquitin ligases (E3s) to transfer Ubiquitin to target proteins such as the cell cycle regulator, p21/CIP1/CDKN1A (5). UbcH8 also regulates actin cytoskeleton dynamics, and dysregulated UbcH8 promotes migration and invasion of human breast cancer cells (6). In addition, UbcH8 interacts with the E3, Parkin, and is thought to play a role in the pathogenesis of Parkinson’s disease (1,7).

References

Kumar, S. et al. (1997) J. Biol. Chem. 272:13548.
Durfee, L.A. & J.M. Huibregste (2012) Methods Mol. Biol. 832:141.
Zhao, C. et al. (2004) Proc. Natl. Acad. Sci. USA 101:7578.
Kim, K. et al. (2004) Mol. Cell. Biol. 24:9592.
Shibata, E. et al. (2011) Mol. Cell. Biol. 31:3136.
Desai, S.D. et al. (2012) Exp. Biol. Med. 237:38.
Zhang, Y. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13354.

Entrez Gene IDs

9246 (Human); 56791 (Mouse); 295704 (Rat)

Alternate Names

EC 6.3.2.19; retinoic acid induced gene B protein; Retinoic acid-induced gene B protein; RIG-B; UbcH8; UBCH8MGC40331; UBE2L6; Ubiquitin carrier protein L6; ubiquitin/ISG15-conjugating enzyme E2 L6; ubiquitin-conjugating enzyme E2L 6; Ubiquitin-protein ligase L6