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Recombinant Human GST-UBE2M/Ubc12 Protein, CF

Catalog #: E2-655 Datasheet / COA / SDS

Discontinued Product

E2-655 has been discontinued.
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Recombinant Human GST-UBE2M/Ubc12 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human GST-UBE2M/Ubc12 is a member of the NEDD8-conjugating (E2) enzyme family that receives NEDD8 from a NEDD8-activating (E1) enzyme and subsequently interacts with a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UBE2M/Ubc12 concentration of 0.1-1 μM.
Source
E. coli-derived human UBE2M/Ubc12 protein
Contains an N-terminal GST (glutathione S-transferase) tag
Accession #
P61081
Predicted Molecular Mass
48 kDa

Product Datasheets

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E2-655

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E2-655

Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.0, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2M/Ubc12

Ubiquitin-conjugating Enzyme E2M (UBE2M), also known as Ubiquitin-conjugating Enzyme 12 (Ubc12), is a ubiquitously expressed protein with a predicted molecular weight of 20 kDa. UBE2M/Ubc12 is highly conserved, and the human ortholog shares 100% amino acid identity with mouse and rat UBE2M. UBE2M/Ubc12 mediates conjugation of the Ubiquitin-like protein Neural Precursor Cell Expressed, Developmentally Down-regulated 8 (NEDD8) to substrate proteins (1). Neddylation requires the coordinated activities of UBE2M/Ubc12 and the APPBPI/UBA3 heterodimer, a NEDD8-activating (E1) enzyme (2,3). The NEDD8 pathway is essential for the viability of many organisms and plays important roles in morphogenesis and cell cycle progression (4-5). UBE2M/Ubc12-dependent neddylation modulates the levels of p27/Kip1, beta-Catenin, Cdc53/Cul1, and other members of the Cullin family (6-9).

References
  1. Gong, L. et al. (1999) J. Biol. Chem. 274:12036. 
  2. Huang, D.T. et al. (2005) Mol. Cell 17:341. 
  3. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
  4. Osaka, F. et al. (2000) EMBO J. 19:3475.
  5. Tateishi, K. et al. (2001) J. Cell Biol. 155:571. 
  6. Huang, A.M. et al. (2011) Biochem. Pharmacol. 82:35.
  7. Miller, B.W. et al. (2009) Mol. Syst. Biol. 5:315.
  8. Huang, D.T. et al. (2009) Mol. Cell 33:483.
  9. Tanaka, T. et al. (2012) Mol. Oncol. 6:267.
Entrez Gene IDs
9040 (Human); 22192 (Mouse); 361509 (Rat)
Alternate Names
EC 6.3.2; EC 6.3.2.-; hUbc12; NEDD8 carrier protein; NEDD8 protein ligase; UBC12; UBC12NEDD8-conjugating enzyme Ubc12; UbcH12; UBC-RS2; UBE2M; ubiquitin carrier protein M; Ubiquitin-conjugating enzyme E2 M; ubiquitin-conjugating enzyme E2M (homologous to yeast UBC12); ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog

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