Home / Ubiquitin E1 Enzyme/UBE1L2 / Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF

Catalog #: E-307
3 Citations Datasheet / COA / SDS

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Catalog # Availability Size / Price Qty
E-307-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6) is a member of the Ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a Ubiquitin-conjugating (E2) enzyme and a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6) concentration of 50-200 nM.
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human Ubiquitin E1 Enzyme/UBE1L2 protein
Met1 - Asp1052 with a N-terminal GST (glutathione S-transferase) tag
Accession #
ABR25253.1
Predicted Molecular Mass
148 kDa

Product Datasheets

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E-307

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-307

Formulation Supplied as a solution in HEPES and DTT. See Certificate of Analysis for details.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ubiquitin E1 Enzyme/UBE1L2

Ubiquitin-activating Enzyme 1-like 2 (UBE1L2), also known as Ubiquitin-like Modifier Activating Enzyme 6 (UBA6), is a 1052 amino acid (aa) member of the Ubiquitin-activating (E1) enzyme family with a predicted molecular weight of 118 kDa. The mouse and rat UBE1L2/UBA6 orthologs share 88% aa sequence identity with the human protein. UBE1L2/UBA6 is expressed in vertebrate cells where four isoforms have been identified (1). UBE1L2/UBA6 is an activating enzyme for both Ubiquitin and FAT10 via a similar molecular mechanism but has a higher affinity for FAT10 than for Ubiquitin (2-4). UBE1L2/UBA6 contains an ATP-binding motif and an active site cysteine residue, Cys625 in humans, common to E1 enzymes. It shares 40% aa sequence identity with the related family member Ubiquitin Activating Enzyme/UBE1 (1). Similar to UBE1, UBE1L2/UBA6 can transfer activated Ubiquitin to Ubiquitin-conjugating (E2) enzymes including the UbcH5 isoforms and UbcH7 (5). Unlike UBE1, which can transfer Ubiquitin to the cell cycle-associated E2 enzyme, UbcH3, UBE1L2/UBA6 can transfer Ubiquitin to Use1/UBE2Z, an E2 enzyme that cannot be charged with Ubiquitin by UBE1 (1,5). UBE1L2/UBA6-initiated covalent linkage of FAT10 to protein targets results in proteasomal degradation (2). UBE1L2/UBA6 is essential for embryonic development (3). One isoform, nUBE1L, is highly expressed in human testis and may play a role in spermatogenesis and male fertility (6).


References
  1. Pelzer, C. et al. (2007) J. Biol. Chem. 282:23010.
  2. Pelzer, C. & M. Groettrup (2010) Subcell. Biochem. 54:238.
  3. Chiu, Y.H. et al. (2007) Mol. Cell 27:1014.
  4. Gavin, J.M. et al. (2012) J. Biol. Chem. 287:15512.
  5. Jin, J. et al. (2007) Nature 447:1135.
  6. Zhu, H. et al. (2004) Acta Biochim. Biophys. Sin. (Shanghai) 36:227.
Long Name
Ubiquitin-activating Enzyme E1-like 2
Alternate Names
MOP4; UBA6; UBEL2; Ubiquitin E1 Enzyme

Citations for Recombinant Human GST-Ubiquitin E1 Enzyme (UBE1L2/UBA6), CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes
    Authors: Yan, W;Zhong, Y;Hu, X;Xu, T;Zhang, Y;Kales, S;Qu, Y;Talley, DC;Baljinnyam, B;LeClair, CA;Simeonov, A;Polster, BM;Huang, R;Ye, Y;Rai, G;Henderson, MJ;Tao, D;Fang, S;
    Nature communications
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  2. Targeting a helix-in-groove interaction between E1 and E2 blocks ubiquitin transfer
    Authors: AM Cathcart, GH Bird, TE Wales, HD Herce, EP Harvey, ZJ Hauseman, CE Newman, U Adhikary, MS Prew, T Oo, S Lee, JR Engen, LD Walensky
    Nat. Chem. Biol., 2020-08-17;0(0):.
    Species: Human, N/A
    Sample Types: Peptide, Protein
    Applications: Bioassay
  3. Identification of the degradation determinants of insulin receptor substrate 1 for signaling cullin-RING E3 ubiquitin ligase 7-mediated ubiquitination.
    Authors: Xu, Xinsong, Keshwani, Malik, Meyer, Kathleen, Sarikas, Antonio, Taylor, Susan, Pan, Zhen-Qia
    J Biol Chem, 2012-10-08;287(48):40758-66.
    Applications: Binding Assay

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