Recombinant Human HAPLN1 Protein, CF Summary
Product Specifications
Asp16-Asn354, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2608-HP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: HAPLN1
HAPLN1 (also known as link protein and CRTL1) is a member of the hyaladherin family of hyaluronic acid (HA) binding proteins. Hyaluronan binding proteins are of two types; those with link modules, and those without. Link modules are 100 amino acid (aa) HA and protein-binding sequences that contain two alpha -helices and two antiparallel beta -sheets (1, 3). There are three categories of link module-containing proteins. “A” domain-type proteins contain one link module; “B” domain-type proteins contain one link module with an N- and C-terminal flanking region; and “C” domain-type proteins have an extended structure with one N-terminal V-type Ig-like domain followed by two link modules (2). The HAPLN family is a group of four C domain-type proteins that share approximately 50% aa identity (4). HAPLN1 is synthesized as a 354 aa precursor that contains a 15 aa signal sequence and a 339 aa mature region (4 - 6). It contains one Ig-like domain and two 95 aa link modules (6). It is variably glycosylated with a native molecular weight between 41 - 48 kDa (7, 8). Mature human HAPLN1 is 97%, 96%, 96%, 96%, and 96% aa identical to equine, porcine, rat, mouse and bovine HAPLN1, respectively. HAPLN1 contributes to extracellular matrix stability and flexibility (9). In cartilage, HALPN1 forms a ternary complex with HA and aggrecan. This creates a gel-like substance with remarkable resistance to deformation (3). In this complex, HA forms a linear backbone with perpendicularly attached aggrecan and HAPLN1. Aggrecan and HAPLN1 lie parallel to each other, while HA runs between the two HAPLN1 link modules (2, 3, 10). The Ig domain of HAPLN1 binds to aggrecan, while the two link modules of HAPLN1 bind to HA. Although HA and aggrecan will associate, the tendency is towards dissociation (2, 3, 8). HAPLN1 provides a stabilizing influence on HA-aggrecan associations, thus creating a long-lived ternary functional complex.
- Day, A.J. and G.D. Prestwich (2002) J. Biol. Chem. 277:4585.
- Seyfried, N.T. et al. (2005) J. Biol. Chem. 280:5435.
- Matsumoto, K. et al. (2003) J. Biol. Chem. 278:41205.
- Spicer, A.P. et al. (2003) J. Biol. Chem. 278:21083.
- Dudhia, J. and T.E. Hardingham (1990) Nucleic Acids Res. 18:1292.
- Osborne-Lawrence, S.L. et al. (1990) Genomics 8:562.
- Roughley, P.J. et al. (1982) J. Biol. Chem. 257:11908.
- Shi, S. et al. (2004) J. Biol. Chem. 279:12060.
- Binette, F. et al. (1994) J. Biol. Chem. 269:19116.
- Perkins, S.J. et al. (1992) Biochem. J. 285:263.
Citations for Recombinant Human HAPLN1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis
Authors: BL Ecker, A Kaur, SM Douglass, MR Webster, FV Almeida, GE Marino, AJ Sinnamon, MG Neuwirth, GM Alicea, A Ndoye, M Fane, X Xu, MS Sim, GB Deutsch, MB Faries, GC Karakousis, AT Weeraratna
Cancer Discov, 2018-10-02;9(1):82-95.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Hyaluronan and proteoglycan link protein 1 (HAPLN1) activates bortezomib-resistant NF-?B activity and increases drug resistance in multiple myeloma
Authors: M Huynh, C Pak, S Markovina, NS Callander, KS Chng, SM Wuerzberge, DD Bakshi, JA Kink, P Hematti, C Hope, F Asimakopou, L Rui, S Miyamoto
J. Biol. Chem., 2017-12-26;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Western Blot -
Protumorigenic role of HAPLN1 and its IgV domain in malignant pleural mesothelioma.
Authors: Ivanova AV, Goparaju CM, Ivanov SV
Clin. Cancer Res., 2009-04-07;15(8):2602-11.
Applications: Western Blot
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