Recombinant Human Hepassocin/FGL1 Fc Chimera Protein, CF

Catalog #: 10573-HE Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10573-HE-050

Recombinant Human Hepassocin/FCL1 Fc Chimera Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Hepassocin/FGL1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human LAG-3 Fc Chimera (Catalog # 2319-L3) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Human Hepassocin/FGL1 Fc Chimera (Catalog # 10573-HE) that produces 50% of the optimal binding response is 0.2-2 μg/mL.

Source

Human embryonic kidney cell, HEK293-derived human Hepassocin/FGL1 protein

MD	Human IgG₁ (Pro100-Lys330)	IEGR	Human Hepassocin/FGF1 (Leu23-Ile312) Accession # Q08830.3
N-terminus			C-terminus

Accession #

Q08830.3

N-terminal Sequence
Analysis

Met

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

61 kDa

SDS-PAGE

60-65 kDa, under reducing conditions

Product Datasheets

10573-HE

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10573-HE

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human LAG-3 Fc Chimera (2319-L3) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Human Hepassocin/FGL1 Fc Chimera (Catalog # 10573-HE) that produces 50% of the optimal binding response is 0.2-2 μg/mL.

SDS-PAGE

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2 μg/lane of 2 μg/lane of Recombinant Human Hepassocin/FGL1 Fc Chimera Protein (Catalog # 10573-HE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60-65 kDa and 120-130 kDa, respectively.

Reconstitution Calculator

Background: Hepassocin/FGL1

Hepassocin, also known as Hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and Fibrinogen-Like Protein 1 (FGL1) (1), is a liver-specific secreted protein belonging to the fibrinogen superfamily whose members share a fibrinogen domain at their C-termini (2). Human Hepassocin/FGL1 is a secreted homodimer consisting of 312 amino acids (aa) with a 22 aa signal sequence and a 290 aa mature protein (3). Mouse and rat Hepassocin/FGL1 share approximately 84% and 83% amino acid identity with human Hepassocin/FGL1, respectively. Human Hepassocin/FGL1 binds LAG-3 through its fibrinogen-like domain independently of MHC class II (4). Hepassocin/FGL1 inhibits antigen-specific T cell activation, with its elevated presence in plasma of cancer patients indicating poor prognoses (4). Other than this role in cancer progression, Hepassocin/FGL1 also has restorative function for liver cells. It is upregulated during liver regeneration following partial hepatectomy (5), and stimulates proliferation of hepatocytes in vivo and improves prognoses with fulminant hepatic failure in rats (6). Its expression is regulated in Hep G2 cells by interleukin-6 (IL-6) and is found in the serum in both bound and unbound states as an acute phase reactant (7).

References

Yamamoto, T. et al. (1993) Biochem. Biophys. Res. Commun. 2:681.
Zhang, S.M. et al. (2008) Innate Immun. 14:175.
Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.
Wang, J. et al. (2019) Cell. 176:334.
Yu, HT. et al. (2009) J. Biol. Chem. 284:13335.
Li, C.Y. et al. (2010) Gut. 59:817.
Liu, Z. and C. Ukomadu. (2008) Biochem. Briophys. Res. Commun. 365:729.

Long Name

Fibrinogen Like 1

Entrez Gene IDs

2267 (Human); 246186 (Rat); 102118020 (Cynomolgus Monkey)

Alternate Names

FGL1; fibrinogen-like 1; fibrinogen-like protein 1; Fibrinogen-like-protein 1; FREP1; Hepassocin; hepatocellular carcinoma-related sequence; Hepatocyte-derived fibrinogen-related protein 1; HFREP-1; HFREP1MGC12455; HP-041; LFIRE1; LFIRE-1; Liver fibrinogen-related protein 1