Home / NEDD8 / Recombinant Human His6-NEDD8 Protein, CF

Recombinant Human His6-NEDD8 Protein, CF

Catalog #: UL-813 Datasheet / COA / SDS

Discontinued Product

UL-813 has been discontinued.
View all NEDD8 products.
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
FAQs
Reviews
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-NEDD8 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human His6-NEDD8 can be conjugated to substrate proteins via the subsequent actions of a NEDD8-activating (E1) enzyme, a NEDD8-conjugating (E2) enzyme, and a NEDD8 ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-NEDD8 concentration of 5-20 μM.
Source
E. coli-derived human NEDD8 protein
Contains a 6-His tag
Accession # Q15843
Accession #
Q15843
Predicted Molecular Mass
9.5 kDa

Product Datasheets

You must select a language.

x

UL-813

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

UL-813

Formulation Supplied as a solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: NEDD8

Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Related to Ubiquitin 1 (Rub1), is a 6-8 kDa member of the Ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one Ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide (1,2). Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from mouse, rat, and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits (3). Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes (4). Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates (5). For example, transfer of NEDD8 to the Cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of Ubiquitin ligases (E3) (6-8). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival (9,10). Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies (11,12).

References
  1. Kumar, S. et al. (1993) Biochem. Biophys. Res. Commun. 195:393.
  2. Kamitani, T. et al. (1997) J. Biol. Chem. 272:28557.
  3. Huang, D.T. & B.A. Schulman (2005) Methods Enzymol. 398:9.
  4. Gong, L. & E.T. Yeh (1999) J. Biol. Chem. 274:12036.
  5. Ohki, Y. et al. (2009) Biochem. Biophys. Res. Commun. 381:443.
  6. Petroski, M.D. & R.T. Deshares (2005) Nat. Rev. Mol. Cell Biol. 6:9.
  7. Hjerpe, R. et al. (2012) Biochem. J. 441:927.
  8. Calabrese, M.F. et al. (2011) Nat. Struct. Mol. Biol. 18:947.
  9. Tateishi, K. et al. (2001) J. Cell. Biol. 155:571.
  10. Ohh, M. et al. (2002) EMBO Rep. 3:177.
  11. Luo, Z. et al. (2012) Cancer Res. 72:3360.
  12. Soucy, T.A. et al. (2010) Genes Cancer 1:708.
Long Name
Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8
Entrez Gene IDs
4738 (Human); 18002 (Mouse); 25490 (Rat)
Alternate Names
FLJ43224; MGC104393; MGC125896; MGC125897; NEDD8; NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Rub1; Ubiquitin-like protein Nedd8

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human His6-NEDD8 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human His6-NEDD8 Protein, CF and earn rewards!

Have you used Recombinant Human His6-NEDD8 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review