Recombinant Human His6-USP4 Protein, CF

Catalog #: E-596 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
E-596-050

R&D Systems Recombinant Proteins and Enzymes

1 Image

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human His6-USP4 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP4 concentration of 10-100 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP4 protein
Met1 - Asn963 with a C-terminal 6-His tag

Accession #

Q13107.3

Predicted Molecular Mass

109 kDa

Product Datasheets

E-596

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-596

Formulation	Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

Background: USP4

Ubiquitin carboxyl-terminal hydrolase 4 (USP4) is a specialized cysteine protease with a predicted molecular weight of 109 kDa. USP4 is a member of the peptidase C19 family and the human protein shares 91% amino acid sequence identity with its mouse ortholog. USP4 is ubiquitinated on multiple sites, and deubiquitination of these sites requires USP4 catalytic activity. USP4 interacts with CtIP protein and the MRN (MRE11-RAD50-NBS1) complex to promote DNA double-strand break repair. Other reported USP4 targets include HDAC2, ADORA2A, PDPK1 and TRIM21.

References

Li, Z., et al. (2016) Oncogene 35:2902.
Milojevic, T., et al. (2006) Mol. Pharmacol. 69:1083.
Wada, K. & T. Kamitani (2006) Biochem. Biophys. Res. Commun. 342:253.
Wijnhoven, P., et al. (2015) Mol. Cell 60:362.

Long Name

Ubiquitin Specific Protease 4

Entrez Gene IDs

7375 (Human); 22258 (Mouse); 290864 (Rat)

Alternate Names

Deubiquitinating enzyme 4; EC 3.1.2.15; EC 3.4.19.12; MGC149848; MGC149849; ubiquitin carboxyl-terminal esterase 4; ubiquitin carboxyl-terminal hydrolase 4; ubiquitin specific peptidase 4 (proto-oncogene); ubiquitin specific protease 4 (proto-oncogene); ubiquitin thioesterase 4; Ubiquitin thiolesterase 4; ubiquitin-specific processing protease 4; Ubiquitin-specific-processing protease 4; Ubiquitous nuclear protein homolog; UNP; Unph; UNPUnph; USP4