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Recombinant Human HMGB1 Protein, CF

Catalog #: 1690-HMB
79 Citations 1 Review Datasheet / COA / SDS
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1690-HMB-050
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Recombinant Human HMGB1 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 2.5-12.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human HMGB1/HMG-1 protein
Met1-Glu215
Accession #
P09429
N-terminal Sequence
Analysis
Met1 & Gly2
Predicted Molecular Mass
25 kDa
SDS-PAGE
28-34 kDa, reducing conditions

Product Datasheets

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1690-HMB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1690-HMB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HMGB1/HMG-1

High-mobility Group Box 1 protein (HMGB1), also known as HMG1 or Amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins (1-3). Human HMGB1 is expressed as a 25 kDa single chain protein containing two globular positively charged DNA-binding domains (HMG boxes A and B) and a negatively charged C-terminal region that contains only Asp and Glu residues (4, 5). Posttranslational modification of HMGB1, including acetylation, phosphorylation, and methylation, affects HMGB1 localization, receptor interactions, and bioactivity (3). An intramolecular disulfide bond between Cys23 and Cys45 as well as the presence of the unpaired Cys106 thiol are critical for HMGB1-induced pro-inflammatory TNF-alpha secretion (2, 6). Alternatively, fully reduced HMGB1 acts as a potent chemoattractant for neutrophils and monocytes (7). HMGB1 can be localized to the nucleus or cytoplasm and can also be secreted despite its lack of a signal peptide (2). HMGB1 binds DNA in a non-sequence specific manner and may act as a structural cofactor during gene transcription (8). Acetylation of HMGB1 results in its cytoplasmic localization and eventual secretion (9). HMGB1 can be secreted by multiple cell types, and it is also released upon cell necrosis, apoptosis, and pyroptosis (2, 3). HMGB1 is widely recognized as a multifunctional alarmin that stimulates inflammation upon sterile or infectious insult. Receptors for HMGB1 include TLR2, TLR4, TLR9, Syndecan-3, Siglec-10, Integrin alpha M beta 2, CXCR4, TIM-3, and RAGE (1, 2). It is implicated in the pathogenesis of a broad range of diseases including atherosclerosis, sepsis, cancer, neurodegenerative diseases, and autoimmunity (3, 10-13).

References
  1. Yanai, H. et al. (2012) Trends Immunol. 33:633.
  2. Yang, H. et al. (2013) J. Leukoc. Biol. 93:865.
  3. Harris, H.E. et al. (2012) Nat. Rev. Rheumatol. 8:195.
  4. Degryse, B. and M. de Virgilio (2003) FEBS Lett. 553:11.
  5. Wen, L. et al. (1989) Nucleic Acids Res. 17:1197.
  6. Yang, H. et al. (2021) Mol. Med. 27:58.
  7. Venereau, E. et al. (2012) J. Exp. Med. 209:1519.
  8. Bianchi, M.E. and A. Agresti (2005) Curr. Opin. Genet. Dev. 15:496.
  9. Bonaldi, T. et al. (2003) EMBO J. 22:5551.
  10. Diener, K.R. et al. (2013) Immunol. Cell Biol. 91:443.
  11. Fang, P. et al. (2012) Mol. Neurobiol. 45:499.
  12. de Souza, A.W. et al. (2012) Autoimmun. Rev. 11:909.
  13. Bae, J.S. (2012) Arch. Pharm. Res. 35:1511.
Long Name
High Mobility Group Protein 1
Entrez Gene IDs
3146 (Human)
Alternate Names
Amphoterin; high mobility group box 1; High mobility group protein 1; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG1; HMG-1; HMG1DKFZp686A04236; HMG3; HMGB1; SBP-1; Sulfoglucuronyl carbohydrate binding protein

Citations for Recombinant Human HMGB1 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

79 Citations: Showing 1 - 10
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  1. High-mobility group box 1 increases platelet surface P2Y12 and platelet activation in sickle cell disease
    Authors: Nolfi-Donegan, D;Annarapu, GK;St Croix, C;Calderon, M;Hillery, CA;Shiva, S;
    JCI insight
    Species: Mouse
    Sample Types: In Vivo
    Applications: In vivo assay
  2. Differential Expression of Circulating Damage-Associated Molecular Patterns in Patients with Coronary Artery Ectasia
    Authors: Tsoporis, JN;Triantafyllis, AS;Kalogeropoulos, AS;Izhar, S;Rigopoulos, AG;Rallidis, LS;Sakadakis, E;Toumpoulis, IK;Salpeas, V;Leong-Poi, H;Parker, TG;Rizos, I;
    Biomolecules
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. Individual cell types in C. elegans age differently and activate distinct cell-protective responses
    Authors: Roux, AE;Yuan, H;Podshivalova, K;Hendrickson, D;Kerr, R;Kenyon, C;Kelley, D;
    Cell reports
    Species: Rat
    Sample Types: In Vivo
    Applications: In vivo assay
  4. RAGE/SNAIL1 signaling drives epithelial-mesenchymal plasticity in metastatic triple-negative breast cancer
    Authors: Pujals, M;Mayans, C;Bellio, C;Méndez, O;Greco, E;Fasani, R;Alemany-Chavarria, M;Zamora, E;Padilla, L;Mitjans, F;Nuciforo, P;Canals, F;Nonell, L;Abad, M;Saura, C;Tabernero, J;Villanueva, J;
    Oncogene
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. HMGB1/STAT3/p65 Axis Drives Microglial Activation and Autophagy Exert a Crucial Role in Chronic Stress-Induced Major Depressive Disorder
    Authors: Xu, K;Wang, M;Wang, H;Zhao, S;Tu, D;Gong, X;Li, W;Liu, X;Zhong, L;Chen, J;Xie, P;
    Journal of advanced research
    Species: Mouse
    Sample Types:
    Applications: In vivo assay
  6. HMGB1-induced activation of ER stress contributes to pulmonary artery hypertension in vitro and in vivo
    Authors: Zhang, Q;Chen, Y;Wang, Q;Wang, Y;Feng, W;Chai, L;Liu, J;Li, D;Chen, H;Qiu, Y;Shen, N;Shi, X;Xie, X;Li, M;
    Respiratory research
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  7. HMGB1 acts as an agent of host defense at the gut mucosal barrier
    Authors: Overstreet, AC;Anderson, B;Burge, M;Zhu, X;Tao, Y;Cham, CM;Michaud, B;Horam, S;Sangwan, N;Dwidar, M;Liu, X;Santos, A;Finney, C;Dai, Z;Leone, VA;Messer, JS;
    bioRxiv : the preprint server for biology
    Species: Human, Bacteria - E. Coli
    Sample Types: Bacteria, Whole Cells
    Applications: Bioassay
  8. High-mobility group box 1 (HMGB1) crosses the BBB Bidirectionally
    Authors: Banks, WA;Hansen, KM;Erickson, MA;Crews, FT;
    Brain, behavior, and immunity
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Targeting IKKbeta Activity to Limit Sterile Inflammation in Acetaminophen-Induced Hepatotoxicity in Mice
    Authors: SH Kim, DE Jung, JY Song, J Jung, JK Jung, H Lee, E Roh, JT Hong, SB Han, Y Kim
    Pharmaceutics, 2023-02-20;15(2):.
    Species: Mouse
    Sample Types: Transfected Whole Cells
    Applications: Bioassay
  10. Anti-Na+/K+-ATPase DR antibody attenuates UUO-induced renal fibrosis through inhibition of Na+/K+-ATPase alpha1-dependent HMGB1 release
    Authors: J Zheng, P Lan, M Li, MC Kang, M Xun, X Ma, M Yan, D Sun, Y Shen, X Fu, X Ding, X Yan, WJ Xue
    International immunopharmacology, 2023-02-09;116(0):109826.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. RAGE activation in macrophages and development of experimental diabetic polyneuropathy
    Authors: S Osonoi, H Mizukami, Y Takeuchi, H Sugawa, S Ogasawara, S Takaku, T Sasaki, K Kudoh, K Ito, K Sango, R Nagai, Y Yamamoto, M Daimon, H Yamamoto, S Yagihashi
    JCI Insight, 2022-12-08;7(23):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  12. Upregulation of REG IV gene in human intestinal epithelial cells by lipopolysaccharide via downregulation of microRNA-24
    Authors: S Takasawa, C Tsuchida, S Sakuramoto, T Uchiyama, M Makino, A Yamauchi, A Itaya-Hiro
    Journal of Cellular and Molecular Medicine, 2022-08-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. Mechanical Compression by Simulating Orthodontic Tooth Movement in an In Vitro Model Modulates Phosphorylation of AKT and MAPKs via TLR4 in Human Periodontal Ligament Cells
    Authors: CE Roth, RB Craveiro, C Niederau, H Malyaran, S Neuss, J Jankowski, M Wolf
    International Journal of Molecular Sciences, 2022-07-22;23(15):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. HMGB1 mediates invasion and PD-L1 expression through RAGE-PI3K/AKT signaling pathway in MDA-MB-231 breast cancer cells
    Authors: K Amornsupak, S Thongchot, C Thinyakul, C Box, S Hedayat, P Thuwajit, SA Eccles, C Thuwajit
    BMC Cancer, 2022-05-24;22(1):578.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Self-extracellular RNA promotes pro-inflammatory response of astrocytes to exogenous and endogenous danger signals
    Authors: S Fischer, E Nasyrov, M Brosien, KT Preissner, HH Marti, R Kunze
    Journal of Neuroinflammation, 2021-11-02;18(1):252.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Indoprofen exerts a potent therapeutic effect against sepsis by alleviating high mobility group box 1-mediated inflammatory responses
    Authors: X Bi, X Yan, B Jiang, J Liang, J Zhou, S Lu, J Liu, L Luo, Z Yin
    Toxicology and Applied Pharmacology, 2021-10-29;433(0):115778.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Role of Glycated High Mobility Group Box-1 in Gastric Cancer
    Authors: S Kishi, Y Nishiguchi, K Honoki, S Mori, R Fujiwara-T, T Sasaki, K Fujii, I Kawahara, K Goto, C Nakashima, A Kido, Y Tanaka, Y Luo, H Kuniyasu
    International Journal of Molecular Sciences, 2021-05-13;22(10):.
    Species: Human
    Sample Types: Small Molecule
    Applications: Bioassay
  18. Local administration of HMGB-1 promotes bone regeneration on the critical-sized mandibular defects in rabbits
    Authors: A Monir, T Mukaibo, ABM Abd El-Aal, T Nodai, T Munemasa, Y Kondo, C Masaki, MA El-Shair, K Matsuo, R Hosokawa
    Scientific Reports, 2021-04-26;11(1):8950.
    Species: Rabbit
    Sample Types: In Vivo
    Applications: Bioassay
  19. HMGB1, anti-HMGB1 antibodies, and ratio of HMGB1/anti-HMGB1 antibodies as diagnosis indicator in fever of unknown origin
    Authors: M Chen, L Zhu, M Xue, R Zhu, L Jing, H Wang, Y Qin
    Scientific Reports, 2021-03-03;11(1):5059.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  20. HMGB1 as a potential biomarker and therapeutic target for severe COVID-19
    Authors: R Chen, Y Huang, J Quan, J Liu, H Wang, TR Billiar, MT Lotze, HJ Zeh, R Kang, D Tang
    Heliyon, 2020-12-07;6(12):e05672.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Effects of Circulating HMGB-1 and Histones on Cardiomyocytes-Hemadsorption of These DAMPs as Therapeutic Strategy after Multiple Trauma
    Authors: B Weber, I Lackner, M Baur, G Fois, F Gebhard, I Marzi, H Schrezenme, B Relja, M Kalbitz
    J Clin Med, 2020-05-11;9(5):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin in?ammation
    Authors: Z Wang, H Zhou, H Zheng, X Zhou, G Shen, X Teng, X Liu, J Zhang, X Wei, Z Hu, F Zeng, Y Hu, J Hu, X Wang, S Chen, J Cheng, C Zhang, Y Gui, S Zou, Y Hao, Q Zhao, W Wu, Y Zhou, K Cui, N Huang, Y Wei, W Li, J Li
    Autophagy, 2020-02-16;0(0):1-24.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  23. Cyclosporine-a attenuates retinal inflammation by inhibiting HMGB-1 formation in rats with type 2 diabetes mellitus
    Authors: P Wang, F Chen, X Zhang
    BMC Pharmacol Toxicol, 2020-02-04;21(1):9.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  24. Cigarette smoke-induced HMGB1 translocation and release contribute to migration and NF-kappaB activation through inducing autophagy in lung macrophages
    Authors: Y Le, Y Wang, L Zhou, J Xiong, J Tian, X Yang, X Gai, Y Sun
    J. Cell. Mol. Med., 2019-11-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  25. TLR9 is essential for HMGB1-mediated post-myocardial infarction tissue repair through affecting apoptosis, cardiac healing, and angiogenesis
    Authors: FY Liu, D Fan, Z Yang, N Tang, Z Guo, SQ Ma, ZG Ma, HM Wu, W Deng, QZ Tang
    Cell Death Dis, 2019-06-17;10(7):480.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Bioassay
  26. Non-cell�autonomous modulation of tyrosine hydroxylase by HMGB1 released from astrocytes in an acute MPTP-induced Parkinsonian mouse model
    Authors: SJ Kim, MJ Ryu, J Han, Y Jang, MJ Lee, X Ju, I Ryu, YL Lee, E Oh, W Chung, JY Heo, GR Kweon
    Lab. Invest., 2019-05-01;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  27. HMGB1 promotes hair growth via the modulation of prostaglandin metabolism
    Authors: JH Hwang, H Chu, Y Ahn, J Kim, DY Kim
    Sci Rep, 2019-04-30;9(1):6660.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Cellular senescence in progenitor cells contributes to diminished remyelination potential in progressive multiple sclerosis
    Authors: AM Nicaise, LJ Wagstaff, CM Willis, C Paisie, H Chandok, P Robson, V Fossati, A Williams, SJ Crocker
    Proc. Natl. Acad. Sci. U.S.A., 2019-03-25;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Ectodomain Shedding of RAGE and TLR4 as a Negative Feedback Regulation in High-Mobility Group Box 1-Activated Aortic Endothelial Cells
    Authors: WS Yang, JJ Kim, MJ Lee, EK Lee, SK Park
    Cell. Physiol. Biochem., 2018-11-29;51(4):1632-1644.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. HMGB1 deficiency reduces H2 O2 -induced oxidative damage in human melanocytes via the Nrf2 pathway
    Authors: K Mou, W Liu, Y Miao, F Cao, P Li
    J. Cell. Mol. Med., 2018-10-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Proinflammatory Effects of Respiratory Syncytial Virus-Induced Epithelial HMGB1 on Human Innate Immune Cell Activation
    Authors: K Rayavara, A Kurosky, S J Stafford, NJ Garg, AR Brasier, RP Garofalo, YM Hosakote
    J. Immunol., 2018-10-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  32. Sensory neuronal sensitisation occurs through HMGB-1/ RAGE and TRPV1 in high glucose conditions
    Authors: SM Bestall, RP Hulse, Z Blackley, M Swift, N Ved, K Paton, N Beazley-Lo, DO Bates, LF Donaldson
    J. Cell. Sci., 2018-07-26;0(0):.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  33. High Mobility Group B-1 (HMGB-1) Promotes Apoptosis of Macrophage-Derived Foam Cells by Inducing Endoplasmic Reticulum Stress
    Authors: H Wu, Z Chen, JZ Chen, LG Pei, J Xie, ZH Wei, LN Kang, L Wang, B Xu
    Cell. Physiol. Biochem., 2018-07-24;48(3):1019-1029.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Porcine reproductive and respiratory syndrome virus induces concurrent elevation of High Mobility Group Box-1 protein and pro-inflammatory cytokines in experimentally infected piglets
    Authors: D Senthilkum, K Rajukumar, M Kumar, S Kalaiyaras, D Shrivastav, M Katare, DD Kulkarni, VP Singh
    Cytokine, 2018-06-10;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  35. High-Mobility Group Box 1-Induced Complement Activation Causes Sterile Inflammation
    Authors: SY Kim, M Son, SE Lee, IH Park, MS Kwak, M Han, HS Lee, ES Kim, JY Kim, JE Lee, JE Choi, B Diamond, JS Shin
    Front Immunol, 2018-04-11;9(0):705.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  36. Reduced-HMGB1 suppresses poly(I:C)-induced inflammation in keratinocytes
    Authors: H Mori, M Murakami, T Tsuda, K Kameda, R Utsunomiya, K Masuda, K Shiraishi, X Dai, M Tohyama, H Nakaoka, K Sayama
    J. Dermatol. Sci., 2018-02-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Deep vein thrombosis in mice is regulated by platelet HMGB1 through release of neutrophil-extracellular traps and DNA
    Authors: MR Dyer, Q Chen, S Haldeman, H Yazdani, R Hoffman, P Loughran, A Tsung, BS Zuckerbrau, RL Simmons, MD Neal
    Sci Rep, 2018-02-01;8(1):2068.
    Species: Mouse
    Sample Types: In Vivo
  38. Broad adsorption of sepsis-related PAMP and DAMP molecules, mycotoxins, and cytokines from whole blood using CytoSorb� sorbent porous polymer beads
    Authors: MC Gruda, KG Ruggeberg, P O'Sullivan, T Guliashvil, AR Scheirer, TD Golobish, VJ Capponi, PP Chan
    PLoS ONE, 2018-01-25;13(1):e0191676.
    Species: Human
    Sample Types: Protein
    Applications: Protein Filtration
  39. Association of HMGB1 with oxidative stress markers and regulators in PDR
    Authors: AM Abu El-Asr, K Alam, M Garcia-Ram, A Ahmad, MM Siddiquei, G Mohammad, A Mousa, G De Hertogh, G Opdenakker, R Simó
    Mol. Vis., 2017-12-05;23(0):853-871.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  40. Piperidylmethyloxychalcone improves immune-mediated acute liver failure via inhibiting TAK1 activity
    Authors: SH Park, JA Kwak, SH Jung, B Ahn, WJ Cho, CY Yun, CS Na, BY Hwang, JT Hong, SB Han, Y Kim
    Exp. Mol. Med., 2017-11-17;49(11):e392.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  41. sRAGE alleviates neutrophilic asthma by blocking HMGB1/RAGE signalling in airway dendritic cells
    Authors: F Zhang, X Su, G Huang, XF Xin, EH Cao, Y Shi, Y Song
    Sci Rep, 2017-10-27;7(1):14268.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  42. TNF-? mRNA is negatively regulated by microRNA-181a-5p in maturation of dendritic cells induced by high mobility group box-1 protein
    Authors: J Zhu, FL Wang, HB Wang, N Dong, XM Zhu, Y Wu, YT Wang, YM Yao
    Sci Rep, 2017-09-25;7(1):12239.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  43. DAMPs Synergize with Cytokines or Fibronectin Fragment on Inducing Chondrolysis but Lose Effect When Acting Alone
    Authors: L Ding, JA Buckwalter, JA Martin
    Mediators Inflamm., 2017-07-18;2017(0):2642549.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  44. High mobility group box-1 contributes to anti-myeloperoxidase antibody-induced glomerular endothelial cell injury through a moesin-dependent route
    Authors: H Deng, C Wang, DY Chang, N Hu, M Chen, MH Zhao
    Arthritis Res. Ther., 2017-06-06;19(1):125.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. High-Mobility Group Box 1 Disrupts Metabolic Function with Cigarette Smoke Exposure in a Ceramide-Dependent Manner
    Authors: OJ Taylor, MO Thatcher, ST Carr, JL Gibbs, AM Trumbull, ME Harrison, DR Winden, MJ Pearson, TS Tippetts, WL Holland, PR Reynolds, BT Bikman
    Int J Mol Sci, 2017-05-20;18(5):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  46. Cold-inducible RNA-binding protein through TLR4 signaling induces mitochondrial DNA fragmentation and regulates macrophage cell death after trauma
    Authors: Z Li, EK Fan, J Liu, MJ Scott, Y Li, S Li, W Xie, TR Billiar, MA Wilson, Y Jiang, P Wang, J Fan
    Cell Death Dis, 2017-05-11;8(5):e2775.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  47. Glycyrrhizin Protects Rats from Sepsis by Blocking HMGB1 Signaling
    Authors: F Zhao, Y Fang, S Deng, X Li, Y Zhou, Y Gong, H Zhu, W Wang
    Biomed Res Int, 2017-04-18;2017(0):9719647.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  48. HMGB1 is upregulated in the airways in asthma and potentiates airway smooth muscle contraction via TLR4
    Authors: L Di Candia, E Gomez, E Venereau, L Chachi, D Kaur, ME Bianchi, RA Challiss, CE Brightling, RM Saunders
    J. Allergy Clin. Immunol, 2017-03-01;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  49. HMGB1 contributes to glomerular endothelial cell injury in ANCA-associated vasculitis through enhancing endothelium-neutrophil interactions
    Authors: C Wang, DY Chang, M Chen, MH Zhao
    J. Cell. Mol. Med, 2017-02-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  50. CD16 is indispensable for antibody-dependent cellular cytotoxicity by human monocytes
    Sci Rep, 2016-09-27;6(0):34310.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  51. Cerebral ischemic post?conditioning induces autophagy inhibition and a HMGB1 secretion attenuation feedback loop to protect against ischemia reperfusion injury in an oxygen glucose deprivation cellular model
    Mol Med Rep, 2016-09-19;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  52. Hemorrhagic shock primes for lung vascular endothelial cell pyroptosis: role in pulmonary inflammation following LPS
    Cell Death Dis, 2016-09-08;7(9):e2363.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  53. High Mobility Group Box Protein 1 Boosts Endothelial Albumin Transcytosis through the RAGE/Src/Caveolin-1 Pathway
    Sci Rep, 2016-08-30;6(0):32180.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  54. Receptor for advanced glycation endproducts signaling cascades are activated in pancreatic fibroblasts, but not in the INS1E insulinoma cell line: Are mesenchymal cells major players in chronic inflammation?
    Islets, 2016-07-14;0(0):0.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  55. HMGB1 Induces Secretion of Matrix Vesicles by Macrophages to Enhance Ectopic Mineralization
    Authors: Qiang Chen
    PLoS ONE, 2016-05-31;11(5):e0156686.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  56. Physiologic concentrations of HMGB1 have no impact on cytokine-mediated eosinophil survival or chemotaxis in response to Eotaxin-2 (CCL24).
    Authors: Dyer, Kimberly, Rosenberg, Helene F
    PLoS ONE, 2015-03-16;10(3):e0118887.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  57. High mobility group box 1 induced human lung myofibroblasts differentiation and enhanced migration by activation of MMP-9.
    Authors: Lee C, Wang C, Lee Y, Tsai Y, Liu J
    PLoS ONE, 2015-02-18;10(2):e0116393.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  58. HMGB1 induces angiogenesis in rheumatoid arthritis via HIF-1alpha activation.
    Authors: Park S, Lee S, Kim H, Lee W, Hong K, Kim C
    Eur J Immunol, 2015-01-15;45(4):1216-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  59. Necrotic cell-derived high mobility group box 1 attracts antigen-presenting cells but inhibits hepatocyte growth factor-mediated tropism of mesenchymal stem cells for apoptotic cell death.
    Authors: Vogel S, Borger V, Peters C, Forster M, Liebfried P, Metzger K, Meisel R, Daubener W, Trapp T, Fischer J, Gawaz M, Sorg R
    Cell Death Differ, 2015-01-09;22(7):1219-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  60. Glycyrrhizin, inhibitor of high mobility group box-1, attenuates monocrotaline-induced pulmonary hypertension and vascular remodeling in rats.
    Authors: Yang P, Kim D, Lee Y, Lee S, Kang W, Chang H, Shin J
    Respir Res, 2014-11-25;15(1):148.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  61. Inhibition of HMGB1-induced angiogenesis by cilostazol via SIRT1 activation in synovial fibroblasts from rheumatoid arthritis.
    Authors: Kim H, Park S, Lee S, Lee H, Lee W, Rhim B, Hong K, Kim C
    PLoS ONE, 2014-08-15;9(8):e104743.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  62. A dual-role of Gu-4 in suppressing HMGB1 secretion and blocking HMGB1 pro-inflammatory activity during inflammation.
    Authors: Zhou H, Ji X, Wu Y, Xuan J, Qi Z, Shen L, Lan L, Li Q, Yin Z, Li Z, Zhao Z
    PLoS ONE, 2014-03-06;9(3):e89634.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  63. Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET.
    Authors: Vogel S, Chatterjee M, Metzger K, Borst O, Geisler T, Seizer P, Muller I, Mack A, Schumann S, Buhring H, Lang F, Sorg R, Langer H, Gawaz M
    J Biol Chem, 2014-02-24;289(16):11068-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  64. The alarmin concept applied to human renal transplantation: evidence for a differential implication of HMGB1 and IL-33.
    Authors: Thierry A, Giraud S, Robin A, Barra A, Bridoux F, Ameteau V, Hauet T, Girard J, Touchard G, Gombert J, Herbelin A
    PLoS ONE, 2014-02-20;9(2):e88742.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  65. Cellular mechanisms of high mobility group 1 (HMGB-1) protein action in the diabetic retinopathy.
    Authors: Santos, Andrea R, Dvoriantchikova, Galina, Li, Yiwen, Mohammad, Ghulam, Abu El-Asrar, Ahmed M, Wen, Rong, Ivanov, Dmitry
    PLoS ONE, 2014-01-31;9(1):e87574.
    Species: Human, Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Bioassay, In Vivo
  66. Cancer usurps skeletal muscle as an energy repository.
    Authors: Luo, Yi, Yoneda, Junya, Ohmori, Hitoshi, Sasaki, Takamits, Shimbo, Kazutaka, Eto, Sachise, Kato, Yumiko, Miyano, Hiroshi, Kobayashi, Tsuyoshi, Sasahira, Tomonori, Chihara, Yoshitom, Kuniyasu, Hiroki
    Cancer Res, 2013-11-06;74(1):330-40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  67. IL-1alpha released from damaged epithelial cells is sufficient and essential to trigger inflammatory responses in human lung fibroblasts.
    Authors: Suwara M, Green N, Borthwick L, Mann J, Mayer-Barber K, Barron L, Corris P, Farrow S, Wynn T, Fisher A, Mann D
    Mucosal Immunol, 2013-10-30;7(3):684-93.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA (Standard)
  68. Endothelial pentraxin 3 contributes to murine ischemic acute kidney injury.
    Authors: Chen, Jianlin, Matzuk, Martin M, Zhou, Xin J, Lu, Christop
    Kidney Int, 2012-08-15;82(11):1195-207.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  69. The persistent release of HMGB1 contributes to tactile hyperalgesia in a rodent model of neuropathic pain.
    J Neuroinflammation, 2012-07-23;9(0):180.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  70. S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis.
    Clin. Cancer Res., 2012-06-20;18(16):4356-64.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  71. TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding.
    Authors: Sakaguchi M, Murata H, Yamamoto K, Ono T, Sakaguchi Y, Motoyama A, Hibino T, Kataoka K, Huh NH
    PLoS ONE, 2011-08-01;6(8):e23132.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  72. Effects of high mobility group box protein-1, interleukin-1beta, and interleukin-6 on cartilage matrix metabolism in three-dimensional equine chondrocyte cultures.
    Authors: Ley C, Svala E, Nilton A, Lindahl A, Eloranta ML, Ekman S, Skioldebrand E
    Connect. Tissue Res., 2010-11-30;52(4):290-300.
    Species: Equine
    Sample Types: Whole Cells
    Applications: Bioassay
  73. Complement C3a, CpG oligos, and DNA/C3a complex stimulate IFN-alpha production in a receptor for advanced glycation end product-dependent manner.
    Authors: Ruan BH, Li X, Winkler AR, Cunningham KM, Kuai J, Greco RM, Nocka KH, Fitz LJ, Wright JF, Pittman DD, Tan XY, Paulsen JE, Lin LL, Winkler DG
    J. Immunol., 2010-09-03;185(7):4213-22.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  74. Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligands.
    Authors: Rao NV, Argyle B, Xu X, Reynolds PR, Walenga JM, Prechel M, Prestwich GD, MacArthur RB, Walters BB, Hoidal JR, Kennedy TP
    Am. J. Physiol., Cell Physiol., 2010-04-07;299(1):C97-110.
    Species: Human, Mouse
    Sample Types: In Vivo, Protein, Recombinant Protein
    Applications: Binding Assay, ELISA (Capture), In Vivo
  75. Hemorrhagic shock augments lung endothelial cell activation: role of temporal alterations of TLR4 and TLR2.
    Authors: Li Y, Xiang M, Yuan Y, Xiao G, Zhang J, Jiang Y, Vodovotz Y, Billiar TR, Wilson MA, Fan J
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009-10-14;297(6):R1670-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  76. Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors.
    Authors: Ghiringhelli F, Apetoh L, Tesniere A, Aymeric L, Ma Y, Ortiz C, Vermaelen K, Panaretakis T, Mignot G, Ullrich E, Perfettini JL, Schlemmer F, Tasdemir E, Uhl M, Genin P, Civas A, Ryffel B, Kanellopoulos J, Tschopp J, Andre F, Lidereau R, McLaughlin NM, Haynes NM, Smyth MJ, Kroemer G, Zitvogel L
    Nat. Med., 2009-09-20;15(10):1170-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  77. Functional dissection of an IFN-alpha/beta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection.
    Authors: Zhou J, Huang JD, Poon VK, Chen DQ, Chan CC, Ng F, Guan XY, Watt RM, Lu L, Yuen KY, Zheng BJ
    J. Hepatol., 2009-05-03;51(2):322-32.
    Applications: Western Blot
  78. Negative inotropic effects of high-mobility group box 1 protein in isolated contracting cardiac myocytes.
    Authors: Tzeng HP, Fan J, Vallejo JG, Dong JW, Chen X, Houser SR, Mann DL
    Am. J. Physiol. Heart Circ. Physiol., 2008-01-25;294(3):H1490-6.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Bioassay
  79. Molecular mechanisms of Ca(2+) signaling in neurons induced by the S100A4 protein.
    Authors: Kiryushko D, Novitskaya V, Soroka V, Klingelhofer J, Lukanidin E, Berezin V, Bock E
    Mol. Cell. Biol., 2006-05-01;26(9):3625-38.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance

FAQs

  1. What is the oxidation state of Recombinant Human HMGB1 Protein, CF (Catalog # 1690-HMB)?

    • The presence of both a disulfide bond between Cys23 and Cys45, as well as an unpaired Cys106 thiol, has been reported in the literature to be critical for HMBG1-induced TNF-alpha secretion. The activity of 1690-HMB is measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. Each new bulk lot of 1690-HMB must exhibit acceptable activity in the TNF-alpha secretion assay to pass our activity specifications. The oxidiation state of 1690-HMB is not a product specification and has not been fully characterized in-house.

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Recombinant Human HMGB1 Protein, CF
By Anonymous on 07/17/2018
Application: In vitro bioactivity in cell culture
In vitro bioactivity in cell culture Recombinant Human HMGB1 Protein, CF 1690-HMB