Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein, CF

Catalog #: 11177-HV Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11177-HV-100

Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein Binding Activity.

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human BTLA (Catalog # 9235-BT) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human HVEM/TNFRSF14 Fc Chimera (Catalog # 11177-HV) binds with an ED₅₀ of 50.0-600 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human HVEM/TNFRSF14 protein

Human HVEM/TNFRSF14 (Pro37-Val202) Accession # Q92956.3	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q92956.3

N-terminal Sequence
Analysis

Pro37

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

44 kDa

SDS-PAGE

55-75 kDa, under reducing conditions.

Product Datasheets

11177-HV

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11177-HV

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant Human BTLA (9235-BT) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein (Catalog # 11177-HV) binds with an ED₅₀ of 50.0-600 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human HVEM/TNFRSF14 Fc Chimera Protein (Catalog # 11177-HV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-75 kDa and 110-150 kDa, respectively.

Reconstitution Calculator

Background: HVEM/TNFRSF14

Herpesvirus entry mediator (HVEM), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily. Originally identified as important for entry of herpes simplex virus (HSV) through recognition of HSV glycoprotein D (gD), HVEM has been shown to be involved in the regulation of T cell activity (1). Mature human HVEM consists of an extracellular domain (ECD) with four cysteine-rich domains (CRD), a transmembrane segment, and a cytoplasmic region with a TRAF interaction domain (2). The ECD of human HVEM shares 51% amino acid sequence identity with mouse HVEM. HVEM is found on the membrane of various cell types, including hematopoietic and non-hematopoietic cells, with higher expression in the lung, kidney, and liver (3). HVEM is a receptor for the TNF ligand LIGHT, Ig superfamily members BTLA and CD160, as well as Lymphotoxin-alpha and is involved in bidirectional signaling as both a signaling receptor and a ligand for inhibitory receptors (1,4). Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation (5,6). In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation (3,6,7). HVEM enhances the development of CD8+ T cell memory and Treg function (8, 9). It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epithelial integrity and host defense (10). HVEM is being investigated as an immune checkpoint inhibitor as it is commonly mutated in lymphomas, while upregulation leads to disease progression and is associated with poor prognosis (11, 12).

References

Steinberg, M.W. et al. (2011) Immunological reviews 244:169.
Liu, W. et al. (2021) J Exp Med. 218:e20211112).
Demerlé C. et al. (2021) Front Oncol. 2021 11:682007.
del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
Heo, S.K. et al. (2006) J. Leukoc. Biol. 79:330.
Cai, G. et al. (2008) Nat. Immunol. 9:176.
Gonzalez, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116.
Tao, R. et al. (2008) J. Immunol. 180:6649.
Steinberg, M.W. et al. (2013) PLoS One 8:e77992.
Shui, J.W. et al. (2012) Nature 488:222.
Aubert, N. et al. (2021) Cancers (Basel) 13:3009.
Boice, M. et al. (2016) Cell. 167:405.

Long Name

Herpesvirus Entry Mediator

Entrez Gene IDs

8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)

Alternate Names

ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEM; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2