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Recombinant Human IFN-alpha A (alpha 2a) Protein

Catalog #: 11100-1
16 Citations 1 Review Datasheet / COA / SDS
Newer Version Available: 10984-IF
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11100-1
R&D Systems Recombinant Proteins and Enzymes
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Citations (16)
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Reviews (1)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IFN-alpha A (alpha 2a) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity

Interferon was titrated with the use of the cytopathic effect inhibition assay as described:

Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.

Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc.Chem. Pharm. 12:39]. The EC50 for interferon in this assay is ~1 U/ml.

The units are determined by use of a Human IFN Alpha A (Hu-IFN- alpha A [2a]) laboratory standard calibrated to the international reference standard for human interferon alpha A (Hu-IFN- alpha A [2a]) provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257 (1-2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].

Source
E. coli-derived human IFN-alpha A protein
Accession #
V00549
Predicted Molecular Mass
19.2 kDa

Product Datasheets

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11100-1

Product Datasheet
COA
SDS

11100-1

Formulation

Phosphate, buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IFN-alpha 2/IFNA2

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name
Interferon alpha 2
Entrez Gene IDs
3440 (Human); 15965 (Mouse); 298213 (Rat)
Alternate Names
alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765

Citations for Recombinant Human IFN-alpha A (alpha 2a) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Replication competent HIV-guided CRISPR screen identifies antiviral factors including targets of the accessory protein Nef
    Authors: Prelli Bozzo, C;Laliberté, A;De Luna, A;Pastorio, C;Regensburger, K;Krebs, S;Graf, A;Blum, H;Volcic, M;Sparrer, KMJ;Kirchhoff, F;
    Nature communications
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Regulation of HHLA2 expression in kidney cancer and myeloid cells
    Authors: Shigemura, T;Perrot, N;Huang, Z;Bhatt, RS;Sheshdeh, AB;Ahmar, NE;Ghandour, F;Signoretti, S;McDermott, DF;Freeman, GJ;Mahoney, KM;
    BMC cancer
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Rescue and in vitro characterization of a divergent TBEV-Eu strain from the Netherlands
    Authors: TE Hoornweg, GJ Godeke, MN Hoogerwerf, PB van Kaster, A de Vries, H Sprong, GMGM Verjans, D van Riel, JHJ Reimerink, B Rockx, CBEM Reusken
    Scientific Reports, 2023-02-18;13(1):2872.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. IFITM proteins promote SARS-CoV-2 infection and are targets for virus inhibition in vitro
    Authors: C Prelli Boz, R Nchioua, M Volcic, L Koepke, J Krüger, D Schütz, S Heller, CM Stürzel, D Kmiec, C Conzelmann, J Müller, F Zech, E Braun, R Gro beta, L Wettstein, T Weil, J Wei beta, F Diofano, AA Rodríguez, S Wiese, D Sauter, J Münch, C Goffinet, A Catanese, M Schön, TM Boeckers, S Stenger, K Sato, S Just, A Kleger, KMJ Sparrer, F Kirchhoff
    Nature Communications, 2021-07-28;12(1):4584.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Identification of poly(ADP-ribose) polymerase 9 (PARP9) as a noncanonical sensor for RNA virus in dendritic cells
    Authors: J Xing, A Zhang, Y Du, M Fang, LJ Minze, YJ Liu, XC Li, Z Zhang
    Nature Communications, 2021-05-11;12(1):2681.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. A novel ACE2 isoform is expressed in human respiratory epithelia and is upregulated in response to interferons and RNA respiratory virus infection
    Authors: C Blume, CL Jackson, CM Spalluto, J Legebeke, L Nazlamova, F Conforti, JM Perotin, M Frank, J Butler, M Crispin, J Coles, J Thompson, RA Ridley, LSN Dean, M Loxham, S Reikine, A Azim, K Tariq, DA Johnston, PJ Skipp, R Djukanovic, D Baralle, CJ McCormick, DE Davies, JS Lucas, G Wheway, V Mennella
    Nature Genetics, 2021-01-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. TRIM8 is required for virus-induced IFN response in human plasmacytoid dendritic cells
    Authors: G Maarifi, N Smith, S Maillet, O Moncorgé, C Chamontin, J Edouard, F Sohm, FP Blanchet, JP Herbeuval, G Lutfalla, JP Levraud, NJ Arhel, S Nisole
    Sci Adv, 2019-11-20;5(11):eaax3511.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  8. Trisomy 21 activates the kynurenine pathway via increased dosage of interferon receptors
    Authors: RK Powers, R Culp-Hill, MP Ludwig, KP Smith, KA Waugh, R Minter, KD Tuttle, HC Lewis, AL Rachubinsk, RE Granrath, M Carmona-Ir, RB Wilkerson, DE Kahn, M Joshi, A Lleó, R Blesa, J Fortea, A D'Alessand, JC Costello, KD Sullivan, JM Espinosa
    Nat Commun, 2019-10-18;10(1):4766.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  9. Prevalence and correlation of cytokine-specific autoantibodies with epidemiological factors and C-reactive protein in 8,972 healthy individuals: Results from the Danish Blood Donor Study
    Authors: JH von Steman, AS Rigas, LW Thørner, DGK Rasmussen, OB Pedersen, K Rostgaard, C Erikstrup, H Ullum, MB Hansen
    PLoS ONE, 2017-06-30;12(6):e0179981.
    Species: Human
    Sample Types: Plasma
    Applications: Control
  10. Rabex-5 is a lenalidomide target molecule that negatively regulates TLR-induced type 1 IFN production
    Proc Natl Acad Sci USA, 2016-09-06;113(38):10625-10630.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. HIV-1-Mediated BAFF Secretion in Macrophages Does Not Require Endosomal TLRs, Type-I IFN, and Nef, but Depends on the Cellular Phenotype Status
    Authors: AM Gomez, M Ouellet, A Deshiere, Y Breton, MJ Tremblay
    J Immunol, 2016-03-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. HIV-1-triggered release of type I IFN by plasmacytoid dendritic cells induces BAFF production in monocytes.
    Authors: Gomez A, Ouellet M, Tremblay M
    J Immunol, 2015-01-30;194(5):2300-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth.
    Authors: Ding S, Khoury-Hanold W, Iwasaki A, Robek M
    PLoS Biol, 2014-01-07;12(1):e1001758.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
    Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
    Nature, 2012-07-26;487(7408):505-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. IFN-alpha induces transcription of hypoxia-inducible factor-1alpha to inhibit proliferation of human endothelial cells.
    Authors: Gerber SA, Pober JS
    J. Immunol., 2008-07-15;181(2):1052-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. PD-L1 is induced in hepatocytes by viral infection and by interferon-alpha and -gamma and mediates T cell apoptosis.
    Authors: Muhlbauer M, Fleck M, Schutz C, Weiss T, Froh M, Blank C, Scholmerich J, Hellerbrand C
    J. Hepatol., 2006-06-16;45(4):520-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Human IFN-alpha A (alpha 2a) Protein
By Anonymous on 06/07/2024
Application: Binding assay/Protein-protein interaction
Binding assay/Protein-protein interaction Recombinant Human IFN-alpha A (alpha 2a) Protein 11100-1