Recombinant Human IFN-epsilon Protein

Name: Recombinant Human IFN-epsilon Protein
Brand: R&D Systems
SKU: 9667-ME
Rating: 4 (1 reviews)

Catalog #: 9667-ME
1 Citation 1 Review Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
9667-ME-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
9667-ME-025

Recombinant Human IFN-epsilon Protein Bioactivity

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All IFN-epsilon Proteins and Enzymes

Product Details

Citations (1)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IFN-epsilon Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED₅₀ for this effect is 100-500 ng/mL.

Source

E. coli-derived human IFN-epsilon protein
Leu22-Arg208 (Cys175Ser), with an N-terminal Met and a C-terminal 6-His tag

Accession #

Q86WN2

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

23 kDa

SDS-PAGE

23 kDa, reducing conditions

Product Datasheets

9667-ME (with carrier)

Product Datasheet

COA

SDS

9667-ME/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9667-ME

Formulation	Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9667-ME/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
Reconstitution	Reconstitute at 250 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Recombinant Human IFN-epsilon (Catalog # 9667-ME) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is 100‑500 ng/mL.

Reconstitution Calculator

Background: IFN-epsilon

Interferon-epsilon (IFN-epsilon) is a secreted, approximately 23 kDa member of the type I interferon family of molecules (1). Mature human IFN-epsilon shares 62% and 60% amino acid sequence identity with rat and mouse IFN-epsilon, respectively. IFN-epsilon signals through IFN‑alpha / beta R1 and IFN‑alpha / beta R2 and contributes to TNF-alpha induced signaling (2, 3). It is constitutively expressed in epithelial cells lining the lung, intestines, testes, and female reproductive tract, and it is further up-regulated in the uterus by estrogen (2-6). IFN-epsilon provides mucosal barrier protection against the pathogens Chlamidia and Herpes simplex virus 2 (2). It induces B cell activation, attraction of CD8⁺ T cells to sites of virus infection and directly mediates protection against viral and bacterial genital infections (5, 7). Differential regulation of genes related to central nervous system by recombinant human IFN-epsilon suggests its role in maintenance of the structure and function of brain (8).

References

Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
Fung, K.Y. et al. (2013) Science 339:1088.
Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
Demers, A. et al. (2014) J. Leukoc. Biol. 96:1101.
Xi, Y. et al. (2012) Mucosal Immunol. 5:610.
Hermant, P. et al. (2013) PLoS One 8:e71320.
Day, S.L. et al. (2008) J. Immunol. 180:7158.
Peng, Fu-Wang et.al. (2007) Protein Expression and Purification 53:356.

Long Name

Interferon epsilon

Entrez Gene IDs

338376 (Human); 230405 (Mouse); 298225 (Rat)

Alternate Names

IFNE; IFN-E; IFNE1PRO655; IFNepsilon; IFN-epsilon; IFNT1; Ifn-tau-11; INFE1; interferon epsilon 1; interferon epsilon; Interferon epsilon-1; interferon tau-1; interferon, epsilon; interferon-epsilon; MGC119018; MGC119020

Related Research Areas

Citation for Recombinant Human IFN-epsilon Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

A sensitive assay for measuring whole-blood responses to type I IFNs
Authors: Gervais, A;Le Floc'h, C;Le Voyer, T;Bizien, L;Bohlen, J;Celmeli, F;Al Qureshah, F;Masson, C;Rosain, J;Chbihi, M;Lévy, R;Castagnoli, R;Rothenbuhler, A;Jouanguy, E;Zhang, Q;Zhang, SY;Béziat, V;Bustamante, J;Puel, A;Bastard, P;Casanova, JL;
Proceedings of the National Academy of Sciences of the United States of America
Species: Human
Sample Types: Whole Cells
Applications: Bioassay