Recombinant Human IFN-lambda 4 Protein, CF Summary
Product Specifications
Ala23-Leu179 with an N-terminal Met
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9165-IF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS. |
| Reconstitution | Reconstitute at 250 μg/mL in water. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Recombinant Human IFN-lambda 4 (Catalog # 9165-IF) exhibits anti-viral activity in HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1.2 μg/mL.
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Background: IFN-lambda 4
Interferon-lambda 4 (IFN-lambda 4) is a secreted, approximately 17 kDa member of the type III interferon family of molecules (1). It is generated by a frameshift mutation based on a TT > ∆G polymorphism (2). The TT allele inactivates expression of IFN-lambda 4 (2). The ∆G allele is associated with reduced clearance of hepatitis C virus and reduced degranulation of CTL, NK, and NKT cells in the liver during hepatitis (2, 3), although IFN- lambda 4 itself shows in vitro activity against hepatitis C virus (4). IFN-lambda 4 is expressed in the liver of some chronic hepatitis C patients (5). It signals through a receptor complex containing IL-28 R/IFN-lambda R1 and IL-10 R beta to induce the expression of several interferon stimulated genes (ISG) (2, 4). Alternative splicing generates additional isoforms with large internal deletions or a substituted C-terminal region.
- Wack, A. et al. (2015) Nat. Immunol. 16:802.
- Prokunina-Olsson, L. et al. (2013) Nat. Genet. 45:164.
- Jouvin-Marche, E. et al. (2014) J. Infec. Dis. 209:1907.
- Hamming, O.J. et al. (2013) EMBO J. 32:3055.
- Amanzada, A. et al. (2013) PLoS One 8:e84026.
Citations for Recombinant Human IFN-lambda 4 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Distinct molecular phenotypes involving several human diseases are induced by IFN-lambda3 and IFN-lambda4 in monocyte-derived macrophages
Authors: M De, A Bhushan, WS Grubbe, S Roy, JL Mendoza, S Chinnaswam
Genes and immunity, 2022-02-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Expression of Interferons Lambda 3 and 4 Induces Identical Response in Human Liver Cell Lines Depending Exclusively on Canonical Signaling
Authors: M Lunova, J Kubovciak, B Smolková, M Uzhytchak, K Michalova, A Dejneka, P Strnad, O Lunov, M Jirsa
International Journal of Molecular Sciences, 2021-03-04;22(5):.
Species: Human
Sample Types: Whole Cell
Applications: Bioassay -
Monocytes differentiated into macrophages and dendritic cells in the presence of human IFN-&lambda3 or IFN-&lambda4 show distinct phenotypes
Authors: M De, A Bhushan, S Chinnaswam
J Leukoc Biol, 2020-11-17;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency
Authors: JH Chung, SH Hong, N Seo, TS Kim, HJ An, P Lee, EC Shin, HM Kim
Cytokine, 2019-08-31;125(0):154833.
Species: Human
Sample Types: Recombinant Protein, Transfected Whole Cells, Whole Cells
Applications: Bioassay, Biolayer Interferometry (BLI) -
Interferon lambda protects the female reproductive tract against Zika virus infection
Authors: EA Caine, SM Scheaffer, N Arora, K Zaitsev, MN Artyomov, CB Coyne, KH Moley, MS Diamond
Nat Commun, 2019-01-17;10(1):280.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IFN-?4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling
Authors: AA Obajemu, N Rao, KA Dilley, JM Vargas, F Sheikh, RP Donnelly, RS Shabman, EG Meissner, L Prokunina-, OO Onabajo
J. Immunol., 2017-10-25;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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