Recombinant Human IGFALS/ALS Protein, CF

Catalog #: 9917-IA Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9917-IA-050

Recombinant Human IGFALS/ALS Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IGFALS/ALS Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. In the presence of Recombinant Human IGFBP-3 (Catalog # 675-B3), immobilized Recombinant Human IGFALS/ALS (0.5 µg/mL, 100 µL/well) can bind Recombinant Human IGF-I (Catalog # 291-G1) with an ED₅₀ of 15-90 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IGFALS/ALS protein
Ala28-Cys605, with a C-terminal 6-His tag

Accession #

P35858-1

N-terminal Sequence
Analysis

Ala28

Predicted Molecular Mass

64 kDa

SDS-PAGE

76-88 kDa, reducing conditions

Product Datasheets

9917-IA

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9917-IA

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	12 months from date of receipt, ≤ -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Immobilized Recombinant Human IGFALS/ALS (Catalog # 9917-IA) binds to Recombinant Human IGF-1 (Catalog # 291-G1) in the presence of Recombinant Human IGFBP-3 (Catalog # 675-B3). The ED₅₀ for the binding is 15-90 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human IGFALS was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 76-88 kDa.

Reconstitution Calculator

Background: IGFALS/ALS

IGFALS (Insulin-like growth factor-binding protein complex acid labile subunit) or ALS (Acid labile subunit) is an 84-86 kDa glycoprotein member of the leucine rich repeat superfamily of molecules. It is secreted by hepatocytes in response to growth hormone stimulation, and forms a 140-150 kDa ternary complex with IGFBP3 (or IGFBP5) and IGF-I (1, 2). This complex increases the half-life of IGF-I and retains it in the circulation for use in multiple tissues. Natural variants of IGFALS/ALS have been found in idiopathic short stature in children (3, 4). Mature human IGFALS/ALS is 578 amino acids (aa) in length (aa 28-605). It contains twenty-one 20-23 aa LRRs that participate in protein-protein interactions. Structural studies have predicted the molecule to be a torus with a cluster of negative charge on the inside (5). Over aa 28-603, human IGFALS/ALS shares 79% and 78% aa identity with mouse and rat IGFALS/ALS, respectively.

References