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Recombinant Human IGFBP-5 Protein, CF

Catalog #: 875-B5
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
875-B5-025
R&D Systems Recombinant Proteins and Enzymes
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Citations (11)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IGFBP-5 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.5‑1.5 µg/mL in the presence of 14 ng/mL rhIGF-II.
Source
Mouse myeloma cell line, NS0-derived human IGFBP-5 protein
Leu21-Glu272, with an N-terminal Met
Accession #
P24593
N-terminal Sequence
Analysis
Glu28 (Major) & Met-Leu21 (Minor)
Predicted Molecular Mass
28-29 kDa
SDS-PAGE
35-37 kDa, reducing conditions

Product Datasheets

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875-B5

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

875-B5

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGFBP-5

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-transitional modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Human IGFBP-5 cDNA encodes a 272 amino acid (aa) residue precursor protein with a putative 20 aa residue signal peptide that is processed to generate the 252 aa residue mature protein that is post-translationally modified by O-glycosylations and serine phosphorylations. IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. IGFBP-5 has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracellular matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment.

References
  1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Long Name
Insulin-like Growth Factor Binding Protein 5
Entrez Gene IDs
3488 (Human); 16011 (Mouse)
Alternate Names
IBP5IBP-5; IGF-binding protein 5; IGFBP5; IGFBP-5; insulin-like growth factor binding protein 5; insulin-like growth factor-binding protein 5

Citations for Recombinant Human IGFBP-5 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. IGFBP5 is an ROR1 ligand promoting glioblastoma invasion via ROR1/HER2-CREB signaling axis
    Authors: W Lin, R Niu, SM Park, Y Zou, SS Kim, X Xia, S Xing, Q Yang, X Sun, Z Yuan, S Zhou, D Zhang, HJ Kwon, S Park, C Il Kim, H Koo, Y Liu, H Wu, M Zheng, H Yoo, B Shi, JB Park, J Yin
    Nature Communications, 2023-03-22;14(1):1578.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Androgens modulate keratinocyte differentiation indirectly through enhancing growth factor production from dermal fibroblasts
    Authors: C Kumtornrut, T Yamauchi, S Koike, S Aiba, K Yamasaki
    J. Dermatol. Sci., 2019-01-26;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Mechanisms of Acquired Resistance to Trastuzumab Emtansine in Breast Cancer Cells
    Authors: G Li, J Guo, BQ Shen, D Bumbaca Ya, MX Sliwkowski, LM Crocker, JA Lacap, GD Lewis Phil
    Mol. Cancer Ther., 2018-04-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Effects of Transport Duration and Environmental Conditions in Winter or Summer on the Concentrations of Insulin-Like Growth Factors and Insulin-Like Growth Factor-Binding Proteins in the Plasma of Market-Weight Pigs
    Authors: E Wirthgen, S Goumon, M Kunze, C Walz, M Spitschak, A Tuchschere, J Brown, C Höflich, L Faucitano, A Hoeflich
    Front Endocrinol (Lausanne), 2018-02-13;9(0):36.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  5. Local Production of Activated Factor X in Atherosclerotic Plaque Induced Vascular Smooth Muscle Cell Senescence
    Authors: F Sanada, J Muratsu, R Otsu, H Shimizu, N Koibuchi, K Uchida, Y Taniyama, S Yoshimura, H Rakugi, R Morishita
    Sci Rep, 2017-12-07;7(1):17172.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Material-induced Senescence (MIS): Fluidity Induces Senescent Type Cell Death of Lung Cancer Cells via Insulin-Like Growth Factor Binding Protein 5
    Authors: SS Mano, K Uto, M Ebara
    Theranostics, 2017-10-17;7(19):4658-4670.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Local application of IGFBP5 protein enhanced periodontal tissue regeneration via increasing the migration, cell proliferation and osteo/dentinogenic differentiation of mesenchymal stem cells in an inflammatory niche
    Authors: N Han, F Zhang, G Li, X Zhang, X Lin, H Yang, L Wang, Y Cao, J Du, Z Fan
    Stem Cell Res Ther, 2017-09-29;8(1):210.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
    Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
    Reprod Biol Endocrinol, 2014-12-04;12(0):123.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori.
    Authors: McCaig C, Duval C, Hemers E, Steele I, Pritchard DM, Przemeck S, Dimaline R, Ahmed S, Bodger K, Kerrigan DD, Wang TC, Dockray GJ, Varro A
    Gastroenterology, 2006-03-06;130(6):1754-63.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006-03-03;449(1):164-70.
    Species: Human
    Sample Types: N/A
    Applications: Enzyme Assay
  11. Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability.
    Authors: Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A
    Biochem. Biophys. Res. Commun., 2005-08-05;333(3):1011-6.
    Species: Human
    Sample Types: Recombinant Protein, Whole Cells
    Applications: Bioassay, Enzyme Assay

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