Recombinant Human IGSF21 His-tag Protein, CF

Catalog #: 10330-S2 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10330-S2-050

Recombinant Human IGSF21 His-tag Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IGSF21 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IGSF21 His-tag (Catalog # 10330-S2) is immobilized at 1 µg/mL (100 µL/well), it binds to Recombinant Human Neurexin 2 alpha Fc Chimera (Catalog # 6636-NX) with an ED₅₀ of 20-120 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IGSF21 protein
Tyr25-Thr455, with a C-terminal 6-His tag

Accession #

Q96ID5.1

N-terminal Sequence
Analysis

Tyr25

Predicted Molecular Mass

49 kDa

SDS-PAGE

61-68 kDa, under reducing conditions

Product Datasheets

10330-S2

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10330-S2

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 1 mg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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When Recombinant IGSF21 His-tag (Catalog # 10330-S2) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Neurexin 2 alpha Isoform 2 Fc Chimera (Catalog # 6636-NX) binds with an ED₅₀ of 20-120 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human IGSF21 His-tag (Catalog # 10330-S2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-68 kDa.

Reconstitution Calculator

Background: IGSF21

IGSF21 (Immunoglobulin superfamily member 21) is an approximately 60 kDa glycoprotein that is a member of the immunoglobulin superfamily. In mouse, two isoforms have been identified. The long isoform is predominantly expressed in the brain, while the short isoform ~35 kDa is expressed in other organs (1). IGSF21 is expressed at both embryonic and postnatal stages (1). Human IGSF21 is synthesized as a 467 amino acid (aa) protein that includes a 24 aa signal peptide. The mature protein, which contains two Ig-like domains, shares 95% aa sequence identity with mouse and rat IGSF21. IGSF21 selectively promotes inhibitory presynaptic differentiation through interaction with presynaptic neurexin2 alpha that may represent an important mechanism of inhibitory synapse development in the brain (1). Thalamic IGSF21 expression is altered in response to FGF8 signaling (2). Genotypes of IGSF21 are also reported to influence the development of diabetic retinopathy (3).

References