Recombinant Human IL-1 RI Protein Summary
Product Specifications
Asp21-Thr332
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
269-1R
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
269-1R/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-1 RI
Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.
An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.
Citations for Recombinant Human IL-1 RI Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Expression and characterization of recombinant IL-1Ra in Aspergillus oryzae as a system
Authors: Mahmoudi Azar, L;Karaman, E;Beyaz, B;Göktan, I;Eyüpo?lu, AE;Kizilel, S;Erman, B;Gül, A;Uysal, S;
BMC biotechnology
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance -
Identification and Validation of Nutrient State-Dependent Serum Protein Mediators of Human CD4+ T Cell Responsiveness
Authors: K Han, K Singh, MJ Rodman, S Hassanzade, Y Baumer, RD Huffstutle, J Chen, J Candia, F Cheung, KER Stagliano, M Pirooznia, TM Powell-Wil, MN Sack
Nutrients, 2021-04-28;13(5):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Detailed mechanistic analysis of gevokizumab, an allosteric anti-IL-1beta antibody with differential receptor-modulating properties.
Authors: Issafras H, Corbin J, Goldfine I, Roell M
J Pharmacol Exp Ther, 2013-11-05;348(1):202-15.
Applications: Surface Plasmon Resonance -
Role of interleukin-1beta and tumor necrosis factor-alpha-dependent expression of cyclooxygenase-2 mRNA in thermal hyperalgesia induced by chronic inflammation in mice.
Authors: Narita M, Shimamura M, Imai S, Kubota C, Yajima Y, Takagi T, Shiokawa M, Inoue T, Suzuki M, Suzuki T
Neuroscience, 2007-11-12;152(2):477-86.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007-10-22;329(1):214-8.
Applications: ELISA (Standard) -
Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells.
Authors: Herrmann-Lavoie C, Rao CV, Akoum A
Endocrinology, 2007-08-16;148(11):5377-84.
Applications: Antibody Absorption, ELISA (Standard), Western Blot -
Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies.
Authors: Grundtman C, Salomonsson S, Dorph C, Bruton J, Andersson U, Lundberg IE
Arthritis Rheum., 2007-02-01;56(2):674-87.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay
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This antigen was used as the immunoassay standard for a sandwich ELISA using the AF269 as both the capture and detection. It worked very well.
