Recombinant Human IL-10 R alpha Avi-tag His-tag Protein, CF

Catalog #: AVI11459 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AVI11459-050

Recombinant Human IL‑10 R alpha Avi-tag His-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-10 R alpha Avi-tag His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Recombinant Human IL-10 R alpha Avi-tag His-tag (Catalog # AVI11459) binds Recombinant Human IL-10 (Catalog # 1064-ILB) with an ED₅₀ of 8.00-96.0 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-10 R alpha protein

Human IL-10 R alpha (His22-Asn235) Accession # Q13651.2	Avi-tag	6-His tag
N-terminus		C-terminus

Accession #

Q13651.2

N-terminal Sequence
Analysis

His22

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

28 kDa

SDS-PAGE

40-55 kDa, under reducing conditions.

Product Datasheets

AVI11459

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11459

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 400 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Recombinant Human IL‑10 R alpha Avi-tag His-tag Protein (Catalog # AVI11459) binds Recombinant Human IL‑10 (1064-ILB) with an ED₅₀ of 8.00-96.0 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human IL‑10 R alpha Avi-tag His-tag Protein (Catalog # AVI11459) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-55 kDa, under reducing conditions.

Reconstitution Calculator

Background: IL-10 R alpha

Interleukin-10 Receptor alpha (IL-10 R alpha ), also known as IL-10 R1, is a 90-110 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1). IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer (2-5). Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6). IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 R alpha, resulting in the recruitment of IL-10 R beta (6-8). IL-10 R beta is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (1). Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection (10). Polymorphisms of human IL-10 R alpha may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature human IL-10 R alpha consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain (12). Within the ECD, human IL-10 R alpha shares 59% aa sequence identity with mouse and rat IL-10 R alpha. Our Avi-tag Biotinylated human IL-10R alpha features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
Manzanillo, P. et al. (2015) Trends Immunol. 36:471.
Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641.
Mannino, M.H. et al. (2015) Cancer Lett. 367:103.
Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
Tan, J.C. et al. (1993) J. Biol. Chem. 268:21053.
Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
Tan, J.C. et al. (1995) J. Biol. Chem. 270:12906.
Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
Fernandez, S. et al. (2004) J. Immunol. 172:2613.
Gruber, S.G. et al. (2008) Eur. J. Immunol. 38:3365.
Liu, Y. et al. (1994) J. Immunol. 152:1821.

Long Name

Interleukin 10 Receptor alpha

Entrez Gene IDs

3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)

Alternate Names

CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL-10 receptor subunit alpha; IL10R alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10Ra; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha