Recombinant Human IL-13 R alpha 1 Fc Avi-tag Protein, CF

Catalog #: AVI10436 Datasheet / COA / SDS

Biotinylated

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AVI10436-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-13 R alpha 1 Fc Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human IL-13 R alpha 1 Fc Chimera Avi-tag (Catalog # AVI10436) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human IL-13 (Catalog # 213-ILB) binds with an ED₅₀ of 10-80 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-13 R alpha 1 protein

Human IL-13 R alpha 1 (Ala27-Thr343) Accession # AAB37127.1	IEGRMD	Human IgG₁ (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

AAB37127.1

N-terminal Sequence
Analysis

Ala27

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

65 kDa

SDS-PAGE

93-103 kDa, under reducing conditions

Product Datasheets

AVI10436

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10436

Formulation	Supplied as a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Binding Activity

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When Biotinylated Recombinant Human IL-13 R alpha 1 Fc Chimera Avi-tag (Catalog # AVI10436) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human IL-13 (213-ILB) binds with an ED₅₀ of 10-80 ng/mL.

Binding Activity

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2 μg/lane of Biotinylated Recombinant Human IL-13 R alpha 1 Fc Chimera Avi-tag (Catalog # AVI10436) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 93-103 kDa and 186-206 kDa, respectively.

Reconstitution Calculator

Background: IL-13 R alpha 1

IL-13 RA1, also known as IL-13R and IL-13 RA, is a type I transmembrane protein. Its cDNA encodes a 427 aa precursor protein, with 322 aa extracellular domain, 24 aa transmembrane domain and 60 aa intracellular domain. Within the extracellular domain, human IL-13 RA1 shares 75% and 74% homology with mouse and rat IL-13 RA1, respectively. IL-13 RA1 expresses ubiquitously in all tissues with the highest level in heart, liver, skeletal muscle and ovary (1). As a receptor, IL-13 RA1 can function alone or as a heterodimer with IL-4R. Although both IL-4 and IL-13 signal through IL-4R/IL-13 RA1 heterodimer, there are distinct differences. IL-4 binds IL-4R with high affinity then binds IL-13 RA1 with low affinity. In contrast, IL-13 binds IL-13 RA1 with decent affinity, then binds IL-4R with high affinity (2). In addition, the N-terminal Fibronectin type III domain (D1) of IL-13 RA1 is only required for the binding of IL-13 not IL-4 (3,4). After binding to IL-4 or IL-13, the Tyr residues in the cytoplasmic domain of IL-13 RA get phosphorylated and then activate signaling proteins including Jak1, Tyk1, Tyk2, IRS-1, and STAT6 (5, 6). Alternative splicing generates soluble iL-13 RA1 missing the transmembrane domain (7). It not only functions as a decoy receptor for IL-13, but also is able to reduce fasting blood glucose, mediated by IL-4 (8). Higher expression of IL-13 RA1 are found in several cancers, often associated with poor prognosis in patients (9-11). Our Avi-tag Biotinylated human IL-13 RA1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Aman M.J. et al. (1996) J. Biol. Chem. 271:29265.
LaPorte S.L. et al. (2008) Cell 132:259.
Arima K. et al. (2005) J. Biol. Chem. 280:24915.
Ito T, et al. (2009) J. Biol. Chem. 284:24289.
Umeshita-Suyama R. et al. (2000) Int. Immunol. 12:1499.
Roy B, et al. (2002) J. Leukoc. Biol. 72:580.
Osawa M, et al. (2000) Immunogenetics 51:974.
Rachmin I, et al. (2017) Am. J. Physiol. Endocrinol. Metab. 313:E663.
Park M.H. et al. (2017) Ann, Surg. Oncol. 24:3780.
Cao H, et al. (2016) Oncotarget 7:61183.
Suzuki A, et al. (2015) Cytokine 75:79.

Long Name

Interleukin 13 Receptor alpha 1

Entrez Gene IDs

3597 (Human); 16164 (Mouse)

Alternate Names

bB128O4.2.1 (interleukin 13 receptor, alpha 1); Cancer/testis antigen 19; CD213a1 antigen; CD213a1; CT19; IL-13 R alpha 1; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; IL13R alpha 1; IL-13R subunit alpha-1; IL13R; IL13RA; IL-13Ra; IL13RA1; IL-13Ra1; IL-13R-alpha-1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; NR4