Recombinant Human IL-17A, Biotinylated Protein

Name: Recombinant Human IL-17A, Biotinylated Protein
Brand: R&D Systems
SKU: BT7955
Price: 458 USD

Catalog #: BT7955
1 Citation Datasheet / COA / SDS

Newer Version Available: BT7955B

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Discontinued Product

BT7955 has been discontinued and is replaced by BT7955B.

Recombinant Human IL-17A, Biotinylated Protein Bioactivity

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All IL-17/IL-17A Proteins and Enzymes

Product Details

Citations (1)

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17A, Biotinylated Protein Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED₅₀ for this effect is 1.5-7.5 ng/mL. Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.12-1.2 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human IL-17/IL-17A protein
Gly24-Ala155

Accession #

Q16552

N-terminal Sequence
Analysis

Gly24

Structure / Form

Disulfide-linked homodimer, Biotinylated via sugars

Predicted Molecular Mass

15 kDa (unlabeled)

SDS-PAGE

14-23 kDa, reducing conditions

Product Datasheets

BT7955 (with carrier)

Product Datasheet

COA

SDS

BT7955/CF (carrier free)

Discontinued Product

BT7955

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:

Scientific Data

Bioactivity

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Both Biotinylated Recombinant Human IL-17A (Catalog # BT7955) and unlabeled Recombinant Human IL-17A (Catalog # 7955-IL) induces IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 1.5-7.5 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional.

Reconstitution Calculator

Background: IL-17/IL-17A

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17A shares 60% amino acid sequence identity with mouse and rat IL-17A (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9-12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides
(9-11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15).
IL-17A has been shown to exert either tumorigenic or anti-tumor effects (16, 17).

References

Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
Yao, Z. et al. (1995) J. Immunol. 155:5483.
Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
Wright, J.F. et al. (2008) J. Immunol. 181:2799.
Rong, Z. et al. (2009) Cell Res. 19:208.
Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
Liang, S.C. et al. (2007) J. Immunol. 179:7791.
O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
Hsu, H. et al. (2008) Nat. Immunol. 9:166.
Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
Wang, L. et al. (2009) J. Exp. Med. 206:1457.
Kryczek, I. et al. (2009) Blood 114:357.

Long Name

Interleukin 17

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Alternate Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

Related Research Areas

Citation for Recombinant Human IL-17A, Biotinylated Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Identification and structure-based drug design of cell-active inhibitors of interleukin 17A at a novel C-terminal site
Authors: ER Goedken, MA Argiriadi, JD Dietrich, AM Petros, N Krishnan, SC Panchal, W Qiu, H Wu, H Zhu, AM Adams, PM Bodelle, L Goguen, PL Richardson, PF Slivka, M Srikumaran, AK Upadhyay, B Wu, RA Judge, A Vasudevan, SM Gopalakris, PB Cox, VS Stoll, C Sun
Scientific Reports, 2022-08-26;12(1):14561.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay