Recombinant Human IL-17E GMP Protein, CF

Name: Recombinant Human IL-17E GMP Protein, CF
Brand: R&D Systems
SKU: 1258-GMP

Catalog #: 1258-GMP Datasheet / COA / SDS

Discontinued Product

1258-GMP has been discontinued.

View all IL-17E/IL-25 products.

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All IL-17E/IL-25 Proteins and Enzymes

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17E GMP Protein, CF Summary

Product Specifications

Purity

>97%, by SDS-PAGE with silver staining, under reducing conditions.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce CXCL1/GRO alpha secretion in HT‑29 human colon adenocarcinoma cells. The ED₅₀ for this effect is 0.25-1.5 ng/mL.

Source

E. coli-derived human IL-17E/IL-25 protein
Tyr33-Gly177, with an N-terminal Met
Manufactured and tested under cGMP guidelines.

Accession #

Q9H293

N-terminal Sequence
Analysis

Met-Tyr-Ser-His-Trp-Pro-Ser-(Cys)-(Cys)-Pro

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

17 kDa (monomer)

Product Datasheets

1258-GMP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1258-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 6 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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GMP-grade Recombinant Human IL‑17E/IL‑25 (Catalog # 1258-GMP) induces CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The ED₅₀ for this effect is 0.25‑1.5 ng/mL.

SDS-PAGE

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1 μg/lane of GMP-grade Recombinant Human IL-17E/IL-25 (Catalog # 1258-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 17 kDa and 31 kDa, respectively.

Mass Spectrometry

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MALDI-TOF analysis of GMP-grade Recombinant Human IL-17E (Catalog # 1258-GMP). The major peak at 33738 corresponds to the measured molecular weight of the intact dimeric protein. The calculated mass for a monomer is 16873 Da.

Reconstitution Calculator

Background: IL-17E/IL-25

The Interleukin‑17 (IL‑17) family of proteins are immunoregulatory cytokines that share a conserved cysteine‑rich region. IL‑17E, which is also known as IL‑25, promotes Th2‑biased immune responses. This is in contrast to other IL‑17 family members which promote Th1- and Th17‑biased inflammation. IL‑25 is an important mediator of allergic reactions and protection against intestinal parasites (1, 2). Mature human IL‑25 shares 80% amino acid sequence identity with mouse and rat IL‑25 (3, 4). During helminth infections and allergic reactions, IL‑25 is locally up‑regulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils (4‑9). It binds to IL‑17 RB but also requires IL‑17 RA to exert its activity (3, 8, 10). IL‑25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL‑4, IL‑5, IL‑13) and reduced production of Th1 and Th17 cytokines (e.g. IFN‑ gamma, IL‑12, IL‑23, IL‑17A, IL‑17F) (4‑6, 8, 9, 11‑15). Airway IL‑25 can be activated by MMP‑7, a protease that is up‑regulated in airway epithelium in response to allergen exposure (16). Cleaved IL‑25 shows enhanced binding to IL‑17 RB and stronger induction of Th2 cytokines (16). The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and anti‑inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration (4, 6, 9, 13). These actions promote protective anti‑helminth immune responses (4, 5) as well as allergic inflammation and airway hyperreactivity (11). The IL‑25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis (12, 15). IL‑25 also promotes vascular endothelial cell proliferation and assembly into tubular structures (7). It supports the integrity of the blood‑brain barrier and limits CD4⁺ T cell infiltration into the brain (17).

References

Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
Iwakura, Y. et al. (2011) Immunity 34:149.
Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
Fort, M.M. et al. (2001) Immunity 15:985.
Zhao, A. et al. (2010) J. Immunol. 185:6921.
Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
Petersen, B.C. et al. (2012) Nat. Med. 18:751.
Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161.
Cao, Q. et al. (2011) J. Am. Soc. Nephrol. 22:1229.
Stock, P. et al. (2009) J. Immunol. 182:5116.
Caruso, R. et al. (2009) Gastroenterology 136:2270.
Goswami, S. et al. (2009) Nat. Immunol. 10:496.
Sonobe, Y. et al. (2009) J. Biol. Chem. 284:31834.

Long Name

Interleukin 17E

Entrez Gene IDs

64806 (Human); 140806 (Mouse); 501996 (Rat)

Alternate Names

IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25

Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing.

R&D Systems' quality focus includes:

Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented processes and QA control of documentation and process changes
Personnel training programs
Raw material testing and vendor qualification/monitoring
Fully validated equipment, processes and test methods
Equipment calibration schedules using a computerized calibration program
Facility maintenance, safety programs and pest control
Material review process for variances
Monitoring of stability over product shelf-life

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their research. The Certificate of Analysis provided contains the following lot specific information:

N-terminal amino acid analysis, SDS-PAGE analysis, and endotoxin level (as determined by LAL assay) performed on each bulk QC lot, not on individual bottlings of each QC lot
Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial bioburden testing (using broth culture, Sabourand's dextrose and blood agar plates with results reported at 3 days and at 7 days)
Host Cell Protein testing performed by ELISA
Mycoplasma testing by ribosomal RNA hybridization assay

Additional testing and documentation requested by the customer can be arranged at an additional cost. Testing may include, but is not limited to, USP <61> bioburden testing, positive identity testing, testing for adventitious agents and testing for residual host cell content.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.

Product Specific Notices

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