Recombinant Human IL-17RC Isoform 1 Fc Chimera Protein, CF

Catalog #: 9284-IL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
9284-IL-050

Recombinant Human IL-17RC Isoform 1 Fc Chimera Protein Bioactivity

1 Image

Product Details

Supplemental Products

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-17RC Isoform 1 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human IL-17RC Isoform 1 Fc Chimera (Catalog # 9284-IL) is coated at 0.5 µg/mL, Recombinant Human IL-17/IL-17A (Catalog # 7955-IL) binds with an ED₅₀ = 0.1-0.6 ng/mL.

Source

Human embryonic kidney cell, HEK293-derived human IL-17 RC protein

Human IL-17 RC Isoform 1 (Leu21-His465) Accession # NP_703190.1	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

NP_703190.1

N-terminal Sequence
Analysis

Leu21

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

76 kDa

SDS-PAGE

95-113 kDa, reducing conditions

Product Datasheets

9284-IL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9284-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

View Larger

When Recombinant Human IL-17 RC Isoform 1 Fc Chimera (Catalog # 9284-IL) is coated at 0.5 µg/mL, Recombinant Human IL-17/IL-17A (Catalog # 7955-IL) binds with an ED₅₀ = 0.1-0.6 ng/mL.

Reconstitution Calculator

Background: IL-17RC

IL-17 receptor C (IL-17RC; also known as IL-17RL and IL-17Rhom) is an 85-110 kDa member of the IL-17 receptor family. IL-17 plays a key role in the development of autoimmunity and inflammation (1, 2). Human IL-17 RC is a type I transmembrane glycoprotein that is expressed on neutrophils, endothelial cells, chondrocytes and osteoblasts, breast and prostatic epithelium, fibroblasts, renal tubular epithelium, and skeletal muscle cells (3-5). Mature human IL-17 RC consists of a 518 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 232 aa cytoplasmic region (6). It is estimated that there are over 90 alternative splice forms, with transmembrane-containing isoforms predominating (4, 7). This recombinant protein corresponds to IL-17 RC isoform 1, the canonical form. Within the ECD, IL-17 RC isoform 1 shares 69% aa sequence identity with mouse IL-17 RC. IL-17 RC is the cognate receptor for IL-17F, and it binds IL-17A with similar affinity (8). IL-17 RC associates wiith IL-17 RA to form a receptor complex for both IL-17A and IL-17F (8-11).

References

Song, X. and Y. Qian (2013) Cytokine 62:175.
Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.
Taylor, P.R. et al. (2014) Nat. Immunol. 15:143.
You, Z. et al. (2007) Neoplasia 9:464.
Kokubu, T. et al. (2008) J. Hictochem. Cytochem. 56:89.
Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
Haudenschild, D.R. et al. (2006) Prostate 66:1268.
Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
Toy, D. et al. (2006) J. Immunol. 177:36.
Kramer, J.M. et al. (2006) J. Immunol. 176:711.
Kramer, J.M. et al. (2007) J. Immunol. 179:6379.

Long Name

Interleukin 17 Receptor C, Transcript Variant 3

Entrez Gene IDs

84818 (Human); 171095 (Mouse)

Alternate Names

FLJ95963; FLJ96005; IL-17 RC; IL-17 receptor C; IL-17 RL; IL17F receptor; IL17RC; IL-17RC; IL17Rhom; IL17RL; IL-17RL; IL17-RL; interleukin 17 receptor C; interleukin-17 receptor C; Interleukin-17 receptor homolog; Interleukin-17 receptor-like protein; MGC10763; zcytoR14