Recombinant Human IL-18 BPa Fc Chimera Avi-tag Protein, CF

Catalog #: AVI119 Datasheet / COA / SDS

Biotinylated

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AVI119-050

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-18 BPa Fc Chimera Avi-tag Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. In a Human IL‑18/IL‑1F4 Antibody (Catalog # MAB91244) coated plate, Biotinylated Recombinant Human IL‑18 BPa Fc Chimera Avi-tag protein binds to Recombinant Human IL‑18/IL‑1F4 (Catalog # 9124-IL) with an ED₅₀ of 1.00-6.00 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-18 BPa protein

Human IL‑18 BPa (Thr31-Gly194) Accession # O95998.2	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Accession #

O95998.2

N-terminal Sequence
Analysis

Thr31

Structure / Form

Biotinylated via Avi-tag

Predicted Molecular Mass

46 kDa

SDS-PAGE

65-76 kDa and 91-101 kDa, under reducing conditions.

Product Datasheets

AVI119

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI119

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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In a Human IL‑18/IL‑1F4 Antibody (MAB91244) coated plate, Biotinylated Recombinant Human IL‑18 BPa Fc Chimera Avi-tag protein (Catalog # AVI119) binds to Recombinant Human IL‑18/IL‑1F4 (9124-IL) with an ED₅₀ of 1.00-6.00 ng/mL

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human IL‑18 BPa Fc Chimera Avi-tag Protein (Catalog # AVI119) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65 - 76 kDa & 91-101 kDa and 130-155 kDa & 180 - 200 kDa respectively.

Reconstitution Calculator

Background: IL-18 BPa

Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP. Our Avi-tag Biotinylated human IL-18 BPa Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
Calderara, S. et al. (2001) Virology 279:22.

Long Name

Interleukin 18 Binding Protein Isoform a

Entrez Gene IDs

10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)

Alternate Names

IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa