Recombinant Human IL-18 BPa Fc Chimera Protein, CF Summary
Product Specifications
| Human IL-18 Bpa (Thr29-Gly192) Accession # AAD17190 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus | |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
119-BP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: IL-18 BPa
Interleukin 18 binding protein (IL-18 BP) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BP bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BP has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPs share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BP. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly up-regulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.
- Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
- Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
- Calderara, S. et al. (2001) Virology 279:22.
Citations for Recombinant Human IL-18 BPa Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Unleashing Natural IL18 Activity Using an Anti-IL18BP Blocker Induces Potent Immune Stimulation and Antitumor Effects
Authors: Menachem, A;Alteber, Z;Cojocaru, G;Fridman Kfir, T;Blat, D;Leiderman, O;Galperin, M;Sever, L;Cohen, N;Cohen, K;Granit, RZ;Vols, S;Frenkel, M;Soffer, L;Meyer, K;Menachem, K;Galon Tilleman, H;Morein, D;Borukhov, I;Toporik, A;Perpinial Shahor, M;Tatirovsky, E;Mizrachi, A;Levy-Barda, A;Sadot, E;Strenov, Y;Eitan, R;Jakobson-Setton, A;Yanichkin, N;Ferre, P;Ophir, E;
Cancer immunology research
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The Roles of IL-18 in a Realistic Partial Body Irradiation with 5% Bone Marrow Sparing (PBI/BM5) Model
Authors: Cui, W;Hull, L;Zizzo, A;Wang, L;Lin, B;Zhai, M;Kumar, VP;Xiao, M;
Toxics
Species: Mouse, Transgenic Mouse
Sample Types: In Vivo
Applications: In Vivo -
Pharmacokinetic Study of rhIL-18BP and Its Effect on Radiation-Induced Cytokine Changes in Mouse Serum and Intestine
Authors: W Cui, L Hull, A Zizzo, L Wang, B Lin, M Zhai, M Xiao
Toxics, 2022-12-30;11(1):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Th22 cells are efficiently recruited in the gut by CCL28 as an alternative to CCL20 but do not compensate for the loss of Th17 cells in treated HIV-1-infected individuals
Authors: M Nayrac, M Requena, C Loiseau, M Cazabat, B Suc, N Carrere, K Barange, L Alric, G Martin-Blo, J Izopet, P Delobel
Mucosal Immunol, 2020-04-28;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin-18 diagnostically distinguishes and pathogenically promotes human and murine macrophage activation syndrome
Authors: ES Weiss, C Girard-Guy, D Holzinger, AA de Jesus, Z Tariq, J Picarsic, EJ Schiffrin, D Foell, AA Grom, S Ammann, S Ehl, T Hoshino, R Goldbach-M, C Gabay, SW Canna
Blood, 2018-01-11;0(0):.
Species: Human
Sample Types: Serum
Applications: Multiplex Standard -
IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD
Authors: E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch
Respir. Res., 2017-08-22;18(1):159.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Characterization of the Inflammasome in Human Kupffer Cells in Response to Synthetic Agonists and Pathogens
J Immunol, 2016-05-25;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Pro-inflammatory interleukin-18 increases Alzheimer's disease-associated amyloid-beta production in human neuron-like cells.
J Neuroinflammation, 2012-08-16;9(0):199.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration.
Authors: Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B
Am. J. Physiol. Heart Circ. Physiol., 2009-06-26;297(2):H874-86.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Yaba monkey tumor virus encodes a functional inhibitor of interleukin-18.
Authors: Nazarian SH, Rahman MM, Werden SJ, Villeneuve D, Meng X, Brunetti C, Valeriano C, Wong C, Singh R, Barrett JW, Xiang Y, McFadden G
J. Virol., 2007-10-24;82(1):522-8.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance -
Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.
Authors: Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Buhrer C
Ann. Neurol., 2005-01-01;57(1):50-9.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo -
IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction.
Authors: Wu C, Sakorafas P, Miller R, McCarthy D, Scesney S, Dixon R, Ghayur T
J. Immunol., 2003-06-01;170(11):5571-7.
Species: Human
Sample Types: Whole Cells
Applications: Binding Assay
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MAB1192 was used as the capture and AF119 was used as the detection antibody for the sandwich ELISA for IL-18PB. The immunoassay standard was 119-BP. Assay had sensitivity of ~10pg/ml.
