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Recombinant Human IL-18/IL-1F4 Protein

Catalog #: 9124-IL
23 Citations 8 Reviews Datasheet / COA / SDS
Analyzed by SEC-MALS

Carrier Free

Catalog # Availability Size / Price Qty
9124-IL-050/CF
9124-IL-500/CF
9124-IL-010/CF

With Carrier

Catalog # Availability Size / Price Qty
9124-IL-050
9124-IL-500
9124-IL-010
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Recombinant Human IL‑18/IL‑1F4 Protein SEC-MALS.
3 Images
Product Details
Citations (23)
FAQs
Reviews (8)
Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-18/IL-1F4 Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is 1.5-9 ng/mL.
Source
E. coli-derived human IL-18/IL-1F4 protein
Tyr37-Asp193
Accession #
Q14116
N-terminal Sequence
Analysis
Tyr37
Predicted Molecular Mass
18 kDa
SDS-PAGE
18 kDa, reducing conditions

Product Datasheets

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9124-IL (with carrier)

Product Datasheet
COA
SDS

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9124-IL/CF (carrier free)

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9124-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9124-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SEC-MALS View Larger

Recombinant Human IL-18 (Catalog # 9124-IL) has a molecular weight (MW) of 17.5 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.

Bioactivity Recombinant Human IL-18/IL-1F4 Protein Bioactivity View Larger

Recombinant Human IL-18 (Catalog # 9124-IL) induces IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells. The ED50 for this effect is 1.5-9 ng/mL.

SDS-PAGE Recombinant Human IL-18/IL-1F4 Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Human IL-18/IL-1F4 (Catalog # 9124-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 18 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-18/IL-1F4

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed
IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells (11).
IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18 (15). Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation (16). A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin (17).

References
  1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
  2. Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
  3. Gu, Y. et al. (1997) Science 275:206.
  4. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  5. Cheung, H. et al. (2005) J. Immunol. 174:5351.
  6. Novick, D. et al. (1999) Immunity 10:127.
  7. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
  8. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  9. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
  10. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
  11. Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
  12. Li, J. et al. (2004) Cell. Immunol. 227:103.
  13. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
  14. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
  15. Ushio, S. et al. (1996) J. Immunol. 156:4274.
  16. Gaggero, A. et al. (2004) Oncogene 23:7552.
  17. Bellora, F. et al. (2012) Eur. J. Immunol. 42:1618.
Long Name
Interleukin 18
Entrez Gene IDs
3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)
Alternate Names
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

Citations for Recombinant Human IL-18/IL-1F4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Alveolar hypoxia induces organ-specific inflammasome-related inflammation in male mouse lungs
    Authors: Udjus, C;Halvorsen, B;Kong, XY;Sagen, EL;Martinsen, M;Yang, K;Løberg, EM;Christensen, G;Skjønsberg, OH;Larsen, KO;
    Physiological reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Regulation of HHLA2 expression in kidney cancer and myeloid cells
    Authors: Shigemura, T;Perrot, N;Huang, Z;Bhatt, RS;Sheshdeh, AB;Ahmar, NE;Ghandour, F;Signoretti, S;McDermott, DF;Freeman, GJ;Mahoney, KM;
    BMC cancer
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. TNF? induced by DNA-sensing in macrophage compromises retinal pigment epithelial (RPE) barrier function
    Authors: Twarog, M;Schustak, J;Xu, Y;Coble, M;Dolan, K;Esterberg, R;Huang, Q;Saint-Geniez, M;Bao, Y;
    Scientific reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells
    Authors: F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber
    bioRxiv : the preprint server for biology, 2023-02-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Differential IL18 signaling via IL18 receptor and Na-Cl co-transporter discriminating thermogenesis and glucose metabolism regulation
    Authors: X Zhang, S Luo, M Wang, Q Cao, Z Zhang, Q Huang, J Li, Z Deng, T Liu, CL Liu, M Meppen, A Vromman, RA Flavell, GS Hotam??l?g, J Liu, P Libby, Z Liu, GP Shi
    Nature Communications, 2022-12-08;13(1):7582.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Bioassay
  6. In�vivo G-CSF treatment activates the GR-SOCS1 axis to suppress IFN-gamma secretion by natural killer cells
    Authors: X Zhao, T Peng, X Cao, Y Hou, R Li, T Han, Z Fan, M Zhao, Y Chang, H Chen, C Li, X Huang
    Cell Reports, 2022-09-13;40(11):111342.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells
    Authors: YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra
    Science Advances, 2022-08-24;8(34):eabn5522.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Imprint of unconventional T cell response in acute hepatitis C persists despite successful early antiviral treatment
    Authors: Y Du, T Khera, B Strunz, K Deterding, D Todt, N Woller, SA Engelskirc, S Hardtke, K Port, A Ponzetta, E Steinmann, M Cornberg, J Hengst, NK Björkström, H Wedemeyer, HepNet Acu
    European Journal of Immunology, 2021-12-13;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Mucosal-Associated Invariant T (MAIT) Cells Are Highly Activated and Functionally Impaired in COVID-19 Patients
    Authors: S Deschler, J Kager, J Erber, L Fricke, P Koyumdzhie, A Georgieva, T Lahmer, JR Wiessner, F Voit, J Schneider, J Horstmann, R Iakoubov, M Treiber, C Winter, J Ruland, DH Busch, PA Knolle, U Protzer, CD Spinner, RM Schmid, M Quante, K Böttcher
    Viruses, 2021-02-03;13(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Human Thymic CD10+ PD-1+ Intraepithelial Lymphocyte Precursors Acquire Interleukin-15 Responsiveness at the CD1a- CD95+ CD28- CCR7- Developmental Stage
    Authors: L Billiet, G Goetgeluk, S Bonte, S De Munter, L De Cock, M Pille, J Ingels, H Jansen, K Weening, F Van Nieuwe, T Kerre, T Taghon, G Leclercq, B Vandekerck
    Int J Mol Sci, 2020-11-20;21(22):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Boosting NAD Level Suppresses Inflammatory Activation of PBMC in Heart Failure
    Authors: B Zhou, DD Wang, Y Qiu, S Airhart, Y Liu, A Stempien-O, KD O'Brien, R Tian
    J. Clin. Invest., 2020-11-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Inflammatory Signals Induce AT2 Cell-Derived Damage-Associated Transient Progenitors that Mediate Alveolar Regeneration
    Authors: J Choi, JE Park, G Tsagkogeor, M Yanagita, BK Koo, N Han, JH Lee
    Cell Stem Cell, 2020-08-03;27(3):366-382.e7.
    Species: Mouse
    Sample Types: Organoid, Organoids
    Applications: Bioassay
  13. Metabolic Reprograming via Deletion of CISH in Human iPSC-Derived NK Cells Promotes In�Vivo Persistence and Enhances Anti-tumor Activity
    Authors: H Zhu, RH Blum, D Bernareggi, EH Ask, Z Wu, HJ Hoel, Z Meng, C Wu, KL Guan, KJ Malmberg, DS Kaufman
    Cell Stem Cell, 2020-06-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. The TL1A-DR3 Axis Selectively Drives Effector Functions in Human MAIT Cells
    Authors: A Sattler, LG Thiel, AH Ruhm, N Souidi, M Seifert, G Herberth, K Kotsch
    J. Immunol., 2019-10-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Tumor-infiltrating mucosal-associated invariant T (MAIT) cells retain expression of cytotoxic effector molecules
    Authors: P Sundström, L Szeponik, F Ahlmanner, M Sundquist, JSB Wong, EB Lindskog, B Gustafsson, M Quiding-Jä
    Oncotarget, 2019-04-19;10(29):2810-2823.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  16. Induction of antiinflammatory purinergic signaling in activated human iNKT cells
    Authors: JC Yu, G Lin, JJ Field, J Linden
    JCI Insight, 2018-09-06;3(17):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Increased NK Cell Function after Cessation of Long-Term Nucleos(t)ide Analogue Treatment in Chronic Hepatitis B Associates with Liver Damage and HBsAg Loss
    Authors: CL Zimmer, F Rinker, C Höner Zu S, MP Manns, H Wedemeyer, M Cornberg, NK Björkström
    J. Infect. Dis., 2018-04-23;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  18. An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease
    Authors: AW Clarke, L Poulton, D Shim, D Mabon, D Butt, M Pollard, V Pande, J Husten, J Lyons, C Tian, AG Doyle
    MAbs, 2018-03-05;0(0):1-43.
    Species: Human
    Sample Types: Whole Blood
    Applications: Bioassay
  19. Early and late changes in natural killer cells in response to ledipasvir/sofosbuvir treatment
    Authors: L Golden-Mas, RH McMahan, MS Kriss, AL Kilgore, L Cheng, RJ Dran, A Wieland, HR Rosen
    Hepatol Commun, 2018-03-01;2(4):364-375.
    Species: Human
    Sample Types:
  20. Continuous treatment with IL-15 exhausts human NK cells via a metabolic defect
    Authors: M Felices, AJ Lenvik, R McElmurry, S Chu, P Hinderlie, L Bendzick, MA Geller, J Tolar, BR Blazar, JS Miller
    JCI Insight, 2018-02-08;3(3):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  21. EBI3 regulates the NK cell response to mouse cytomegalovirus infection
    Authors: H Jensen, SY Chen, L Folkersen, GP Nolan, LL Lanier
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Mass cytometry analytical approaches reveal cytokine-induced changes in natural killer cells
    Authors: Elena Vendrame
    Cytometry B Clin Cytom, 2017-01-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Selective IRAK4 Inhibition Attenuates Disease in Murine Lupus Models and Demonstrates Steroid Sparing Activity
    Authors: Shailesh Dudhgaonka
    J. Immunol, 2016-12-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

FAQs

  1. What is the difference between the two versions of Recombinant Human IL-18, Catalog # 9124-IL and Catalog # B001-5?

    • Catalog # 9124-IL is manufactured by R&D Systems, while Catalog # B001-5 is manufactured by MBL.  Catalog # 9124-IL is lyophilized powder whereas Catalog # B001-5 is supplied in solution (PBS with BSA and sucrose). Activity of the two proteins is measured in similar cell-based assays; please consult the respective product-specific pages for assay details.  Catalog # 9124-IL is competitively priced and has higher purity and lower endotoxin than Catalog # B001-5.

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Reviews for Recombinant Human IL-18/IL-1F4 Protein

Average Rating: 4.5 (Based on 8 Reviews)

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Recombinant Human IL-18/IL-1F4 Protein
By Anonymous on 07/24/2023
Application: Immunoassay Standard
Reason for Rating: Worked nicely in human serum samples in elisa method
Immunoassay Standard Recombinant Human IL-18/IL-1F4 Protein 9124-IL
Recombinant Human IL-18/IL-1F4 Protein
By Anonymous on 02/02/2023
Application: Tumor Spheroid kill
Other Recombinant Human IL-18/IL-1F4 Protein 9124-IL
Recombinant Human IL-18/IL-1F4 Protein
By Anonymous on 10/26/2020
Application: In vitro bioactivity in cell culture
Reason for Rating: it works great in my assay

I am looking at the inhibition of IFN-r production by the compound with the presence of IL23 and IL-18.

In vitro bioactivity in cell culture Recombinant Human IL-18/IL-1F4 Protein 9124-IL
Recombinant Human IL-18/IL-1F4 Protein
By Anonymous on 10/08/2018
Application: In vitro bioactivity in cell culture
Recombinant Human IL-18/IL-1F4 Protein
By Anonymous on 08/06/2018
Application: Enzymatic activity in vitro
Recombinant Human IL-18/IL-1F4 Protein
By Greta Beckerle on 12/22/2017
Application: Media additive for protein or antibody production
Recombinant Human IL-18/IL-1F4 Protein
By Anonymous on 12/07/2017
Application: Immunoassay Standard

Performed similarly to Competitor1 and Competitor2 proteins in immunoassay

Recombinant Human IL-18/IL-1F4 Protein
By Greta Beckerle on 12/04/2017
Application: In vitro bioactivity in cell culture