Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Protein, CF

Name: Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 872-RP
Rating: 4 (1 reviews)

Catalog #: 872-RP
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
872-RP-100

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant human (rh) IL-1F5, rhIL-1F8, rhIL-1F6 or rhIL-1F9 at 1 µg/mL (100 µL/well) can bind rhIL-1Rrp2 Fc Chimera with a linear range of 0.15-5 µg/mL.

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IL-1Rrp2/IL-1R6 protein

Human IL-1 R6 (Asp20-Tyr337) Accession # Q9HB29	DIEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Accession #

Q9HB29

N-terminal Sequence
Analysis

Asp20

Structure / Form

Disulfide-linked homodimer

Predicted Molecular Mass

62.8 kDa (monomer)

SDS-PAGE

80 kDa, reducing conditions

Product Datasheets

872-RP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

872-RP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-1Rrp2/IL-1R6

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL-1 R4), IL-18 Ra (IL-1 Rrp/IL-1 R5), IL-1Rrp2 (IL-1 RL2/IL-1R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Human IL-1Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 318 aa extracellular domain. It shares 67% and 65% amino acid sequence identity with rat and mouse IL 1Rrp2, respectively. IL-1Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, monocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL-1Rrp2 has been shown to mediate the activation of the transcription factor NF kappa B by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1 epsilon ), with IL-1RAcP as a cofactor (3, 4). Response to IL-1F9 is specifically antagonized by IL-1 F5 (also known as IL-1δ), an IL-1 family ligand that is most closely related to IL-1ra (3). IL-1Rrp2, IL-1 F5, and IL-1F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1Rrp2 mediated signaling pathway may take part in local inflammatory responses (3).

References

Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Lovenberg, T. W. et al. (1996) J. Neuroimmunol. 70:113.
Debets, R. et al. (2001) J. Immunol. 167:1440.
Towne, J. E. et al. (2004) J. Biol. Chem. 279:13677.

Long Name

Interleukin 1 Receptor-like 2

Entrez Gene IDs

8808 (Human); 107527 (Mouse); 171106 (Rat)

Alternate Names

IL-1 R6; IL-1 Rrp2; IL1RL2; IL-1RL2; IL1Rrp2; IL-1Rrp2; IL1RRP2IL1R-rp2Interleukin-1 receptor-related protein 2; interleukin 1 receptor-like 2; interleukin-1 receptor-like 2

Related Research Areas

IL-1 Family

Citation for Recombinant Human IL-1Rrp2/IL-1R6 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

IL-38 binds to the IL-36 receptor and has biological effects on immune cells similar to IL-36 receptor antagonist.
Authors: van de Veerdonk FL, Stoeckman AK, Wu G, Boeckermann AN, Azam T, Netea MG, Joosten LA, Van der Meer JW, Hao R, Kalabokis V, Dinarello CA
Proc. Natl. Acad. Sci. U.S.A., 2012-02-06;109(8):3001-5.
Species: Human
Sample Types: Recombinant Protein
Applications: Binding Assay