Recombinant Human IL-29/IFN-lambda 1 Protein
Recombinant Human IL-29/IFN-lambda 1 Protein Summary
Product Specifications
Gly20-Thr200, with a C-terminal 10-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
1598-IL
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
1598-IL/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-29/IFN-lambda 1
IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13% aa sequence identity) and the type I IFN family (15-19% aa sequence identity) (1-3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon, The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor alpha (IL‑28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory region of the target genes. Human IL-29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively.
- Vilcek, J. (2003) Nature Immunol. 4:8.
- Sheppard, P. et al. (2003) Nature Immunol. 4:63.
- Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
Citations for Recombinant Human IL-29/IFN-lambda 1 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Airway epithelial CD47 plays a critical role in inducing influenza virus-mediated bacterial super-infection
Authors: Moon, S;Han, S;Jang, IH;Ryu, J;Rha, MS;Cho, HJ;Yoon, SS;Nam, KT;Kim, CH;Park, MS;Seong, JK;Lee, WJ;Yoon, JH;Chung, YW;Ryu, JH;
Nature communications
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
Oral Antiviral Defense: Saliva- and Beverage-like Hypotonicity Dynamically Regulate Formation of Membraneless Biomolecular Condensates of Antiviral Human MxA in Oral Epithelial Cells
Authors: Sehgal, PB;Yuan, H;Centone, A;DiSenso-Browne, SV;
Cells
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
The E3 ligase subunit FBXO45 binds the interferon-lambda receptor and promotes its degradation during influenza virus infection
Authors: M Tsai, W Osman, J Adair, R ElMergawy, L Chafin, F Johns, D Farkas, A Elhance, J Londino, RK Mallampall
The Journal of Biological Chemistry, 2022-11-13;0(0):102698.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inhibiting Type I arginine methyltransferase activity promotes the T cell mediated antitumor immune response
Authors: A Fedoriw, L Shi, S O'Brien, KN Smitheman, Y Wang, J Hou, C Sherk, S Rajapurkar, J Laraio, LJ Williams, C Xu, G Han, Q Feng, MT Bedford, L Wang, O Barbash, RG Kruger, P Hwu, HP Mohammad, W Peng
Cancer Immunology Research, 2022-04-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Astrocyte Control of Zika Infection Is Independent of Interferon Type I and Type III Expression
Authors: M Das, ML Smith, T Furihata, S Sarker, R O'Shea, KJ Helbig
Biology, 2022-01-15;11(1):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Delayed induction of type I and III interferons mediates nasal epithelial cell permissiveness to SARS-CoV-2
Authors: CF Hatton, RA Botting, ME Dueñas, IJ Haq, B Verdon, BJ Thompson, JS Spegarova, F Gothe, E Stephenson, AI Gardner, S Murphy, J Scott, JP Garnett, S Carrie, J Powell, CMA Khan, L Huang, R Hussain, J Coxhead, T Davey, AJ Simpson, M Haniffa, S Hambleton, M Brodlie, C Ward, M Trost, G Reynolds, CJA Duncan
Nature Communications, 2021-12-07;12(1):7092.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Intracellular lipid droplet accumulation occurs early following viral infection and is required for an efficient interferon response
Authors: EA Monson, KM Crosse, M Duan, W Chen, RD O'Shea, LM Wakim, JM Carr, DR Whelan, KJ Helbig
Nature Communications, 2021-07-14;12(1):4303.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interferon Lambda Delays the Emergence of Influenza Virus Resistance to Oseltamivir
Authors: C Medaglia, AC Zwygart, PJ Silva, S Constant, S Huang, F Stellacci, C Tapparel
Microorganisms, 2021-06-01;9(6):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Tofacitinib downregulates antiviral immune defence in keratinocytes and reduces T cell activation
Authors: HC Hawerkamp, A Domdey, L Radau, P Sewerin, P Oláh, B Homey, S Meller
Arthritis Research & Therapy, 2021-05-21;23(1):144.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Systematic functional analysis of SARS-CoV-2 proteins uncovers viral innate immune antagonists and remaining vulnerabilities
Authors: M Hayn, M Hirschenbe, L Koepke, R Nchioua, JH Straub, S Klute, V Hunszinger, F Zech, C Prelli Boz, W Aftab, MH Christense, C Conzelmann, JA Müller, S Srinivasac, CM Stürzel, I Forne, S Stenger, KK Conzelmann, J Münch, FI Schmidt, D Sauter, A Imhof, F Kirchhoff, KMJ Sparrer
Cell Reports, 2021-04-27;35(7):109126.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Human Intestinal Organoids Recapitulate Enteric Infections of Enterovirus and Coronavirus
Authors: X Zhao, C Li, X Liu, MC Chiu, D Wang, Y Wei, H Chu, JP Cai, I Hau-Yee Ch, K Kak-Yuen W, J Fuk-Woo Ch, K Kai-Wang T, KY Yuen, J Zhou
Stem Cell Reports, 2021-02-12;16(3):493-504.
Species: Primate - C. aethiops
Sample Types: Whole Cells
Applications: Bioassay -
Interferon-&lambda Enhances the Differentiation of Naive B Cells into Plasmablasts via the mTORC1 Pathway
Authors: M Syedbasha, F Bonfiglio, J Linnik, C Stuehler, D Wüthrich, A Egli
Cell Rep, 2020-10-06;33(1):108211.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Heatr9 is an infection responsive gene that affects cytokine production in alveolar epithelial cells
Authors: CJ Stairiker, M van Meurs, LG Leon, AA Brouwers-H, L Rijsbergen, YM Mueller, PD Katsikis
PLoS ONE, 2020-07-17;15(7):e0236195.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interferon-Dependent and Respiratory Virus-Specific Interference in Dual Infections of Airway Epithelia
Authors: M Essaidi-La, J Geiser, S Huang, S Constant, L Kaiser, C Tapparel
Sci Rep, 2020-06-24;10(1):10246.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Dendritic cells-derived interferon-lambda1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis
Authors: Y Chen, Y Wang, R Tang, J Yang, C Dou, Y Dong, D Sun, C Zhang, L Zhang, Y Tang, Q Dai, F Luo, J Xu, S Dong
Cell Death Dis, 2020-06-02;11(6):414.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo -
RIG-I-like receptor activation drives type I IFN and antiviral signaling to limit�Hantaan orthohantavirus replication
Authors: AM Kell, EA Hemann, JB Turnbull, M Gale
PLoS Pathog., 2020-04-24;16(4):e1008483.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Structure-based glycoengineering of interferon lambda 4 enhances its productivity and anti-viral potency
Authors: JH Chung, SH Hong, N Seo, TS Kim, HJ An, P Lee, EC Shin, HM Kim
Cytokine, 2019-08-31;125(0):154833.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Type III interferon signaling restricts enterovirus 71 infection of goblet cells
Authors: C Good, AI Wells, CB Coyne
Sci Adv, 2019-03-06;5(3):eaau4255.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
MicroRNA-122 supports robust innate immunity in hepatocytes by targeting the RTKs/STAT3 signaling pathway
Authors: H Xu, SJ Xu, SJ Xie, Y Zhang, JH Yang, WQ Zhang, MN Zheng, H Zhou, LH Qu
Elife, 2019-02-08;8(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
REDD1/Autophagy Pathway Is Associated with Neutrophil-Driven IL-1? Inflammatory Response in Active Ulcerative Colitis
Authors: I Angelidou, A Chrysantho, A Mitsios, S Arelaki, A Arampatzio, K Kambas, D Ritis, V Tsironidou, I Moschos, V Dalla, D Stakos, G Kouklakis, I Mitroulis, K Ritis, P Skendros
J. Immunol., 2018-04-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
A systematic approach to the development of a safe live attenuated Zika vaccine
Authors: SS Kwek, S Watanabe, KR Chan, EZ Ong, HC Tan, WC Ng, MTX Nguyen, ES Gan, SL Zhang, KWK Chan, JH Tan, OM Sessions, M Manuel, J Pompon, C Chua, S Hazirah, K Tryggvason, SG Vasudevan, EE Ooi
Nat Commun, 2018-03-12;9(1):1031.
Species: Primate - Chlorocebus aethiops (African Green Monkey)
Sample Types: Whole Cells
Applications: Cell Culture -
Interferon lambda receptor 1 (IFNL1R) transcript is highly expressed in rhinovirus bronchiolitis and correlates with disease severity
Authors: A Pierangeli, M Statzu, R Nenna, L Santinelli, L Petrarca, A Frassanito, M Gentile, G Antonelli, F Midulla, C Scagnolari
J. Clin. Virol., 2018-03-10;102(0):101-109.
Species: Human
Sample Types: Whole Cells
Applications: Stimulation -
IFN-?4 Attenuates Antiviral Responses by Enhancing Negative Regulation of IFN Signaling
Authors: AA Obajemu, N Rao, KA Dilley, JM Vargas, F Sheikh, RP Donnelly, RS Shabman, EG Meissner, L Prokunina-, OO Onabajo
J. Immunol., 2017-10-25;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface
Authors: J Corry, N Arora, CA Good, Y Sadovsky, CB Coyne
Proc. Natl. Acad. Sci. U.S.A., 2017-08-07;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Generation and characterization of interferon-lambda 1-resistant H1N1 influenza A viruses
Authors: NA Ilyushina, VY Lugovtsev, AP Samsonova, FG Sheikh, NV Bovin, RP Donnelly
PLoS ONE, 2017-07-27;12(7):e0181999.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IFN-?4 potently blocks IFN-? signalling by ISG15 and USP18 in hepatitis C virus infection
Authors: PS Sung, SH Hong, JH Chung, S Kim, SH Park, HM Kim, SK Yoon, EC Shin
Sci Rep, 2017-06-19;7(1):3821.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IFN-?1 with Th17 axis cytokines and IFN-? define different subsets in systemic lupus erythematosus (SLE)
Authors: V Oke, S Brauner, A Larsson, J Gustafsson, A Zickert, I Gunnarsson, E Svenungsso
Arthritis Res. Ther., 2017-06-15;19(1):139.
Species: Human
Sample Types: Serum
Applications: ELISA (Standard) -
Interferon (IFN)-? is a potential mediator in lupus nephritis
Authors: Iva Gunnarsson
Lupus Sci Med, 2016-11-22;3(1):e000170.
Species: Human
Sample Types: Serum
Applications: ELISA (Standard) -
Hepatitis-C-virus-induced microRNAs dampen interferon-mediated antiviral signaling
Nat. Med., 2016-11-14;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Dengue virus infection induces interferon-lambda1 to facilitate cell migration
Sci Rep, 2016-07-26;6(0):24530.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Antagonistic antiviral activity between IFN-lambda and IFN-alpha against lethal Crimean-Congo hemorrhagic fever virus in vitro.
Authors: Bordi L, Lalle E, Caglioti C, Travaglini D, Lapa D, Marsella P, Quartu S, Kis Z, Arien K, Huemer H, Meschi S, Ippolito G, Di Caro A, Capobianchi M, Castilletti C
PLoS ONE, 2015-02-18;10(2):e0116816.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Epigenetic reprogramming of the type III interferon response potentiates antiviral activity and suppresses tumor growth.
Authors: Ding S, Khoury-Hanold W, Iwasaki A, Robek M
PLoS Biol, 2014-01-07;12(1):e1001758.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-27, a cytokine, and IFN-lambda1, a type III IFN, are coordinated to regulate virus replication through type I IFN.
Authors: Cao Y, Zhang R, Zhang W, Zhu C, Yu Y, Song Y, Wang Q, Bai L, Liu Y, Wu K, Wu J
J Immunol, 2013-12-11;192(2):691-703.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors.
Authors: Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J
Nature, 2012-07-26;487(7408):505-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans.
Authors: Popugaeva E, Witkowski PT, Schlegel M, Ulrich RG, Auste B, Rang A, Kruger DH, Klempa B
PLoS ONE, 2012-04-24;7(4):e35587.
Species: Human
Sample Types: Whole Cells
Applications: Control -
Adenoviruses-mediated transduction of human oesophageal carcinoma cells with the interferon-lambda genes produced anti-tumour effects.
Authors: Li Q, Kawamura K, Okamoto S, Fujie H, Numasaki M, Namba M, Nagata M, Shimada H, Kobayashi H, Tagawa M
Br. J. Cancer, 2011-09-27;105(9):1302-12.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus.
Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J
J. Invest. Dermatol., 2010-08-19;131(1):133-40.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
Authors: Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K
Genes Immun., 2009-10-01;10(8):702-14.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages.
Authors: Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P
Int. J. Cancer, 2009-07-15;125(2):367-73.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Inhibition of S100A11 gene expression impairs keratinocyte response against vaccinia virus through downregulation of the IL-10 receptor 2 chain.
Authors: Bin L, Howell MD, Kim BE, Hall CF, Streib JE, Leung DY
J. Allergy Clin. Immunol., 2009-07-03;124(2):270-7, 277.e1.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Evidence of a role for B cell-activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps.
Authors: Kato A, Peters A, Suh L, Carter R, Harris KE, Chandra R, Conley D, Grammer LC, Kern R, Schleimer RP
J. Allergy Clin. Immunol., 2008-04-14;121(6):1385-92, 1392.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Maturing dendritic cells are an important source of IL-29 and IL-20 that may cooperatively increase the innate immunity of keratinocytes.
Authors: Wolk K, Witte K, Witte E, Proesch S, Schulze-Tanzil G, Nasilowska K, Thilo J, Asadullah K, Sterry W, Volk HD, Sabat R
J. Leukoc. Biol., 2008-02-15;83(5):1181-93.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
TLR3- and Th2 cytokine-dependent production of thymic stromal lymphopoietin in human airway epithelial cells.
Authors: Kato A, Favoreto S, Avila PC, Schleimer RP
J. Immunol., 2007-07-15;179(2):1080-7.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Biological activity of interleukins-28 and -29: comparison with type I interferons.
Authors: Meager A, Visvalingam K, Dilger P, Bryan D, Wadhwa M
Cytokine, 2005-07-21;31(2):109-18.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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The recombinant IFN-lambda served as the calibration material in an ELISA which used MAB15981 and BAF1598 as matched pair antibodies.