Recombinant Human IL-31 Protein Summary
Product Specifications
Ser24-Thr164
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2824-IL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
2824-IL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-31
Human Interleukin-31 (IL-31) is a 24 kDa, short-chain member of the alpha -helical family of cytokines. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein (1, 2). The mature region shows four alpha -helices which would be expected to show a typical up‑up‑down‑down topology. Human and mouse IL-31 share 24% aa sequence identity in the mature region (1). IL-31 is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. IL-31 signals via a heterodimeric receptor complex composed of a 120 kDa, gp130-related molecule termed IL‑31 RA (also GPL and GLM-R) and the 180 kDa oncostatin M receptor (OSM R beta ) (2-6). In the complex, IL-31 directly binds to GPL, not OSM R (2, 3). IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway (2-5). Although multiple isoforms of IL-31 RA are known, only a form that contains the entire length of the cytoplasmic domain is signaling-capable (2, 3). The IL-31 receptor is constitutively expressed by keratinocytes and up‑regulated by IFN-gamma on monocytes (1). Studies using transgenic mice indicate that IL-31 may contribute to the pruritis (itching) associated with nonatopic dermatitis (1, 7).
- Dillon, S.R. et al. (2004) Nat. Immunol. 5:752.
- Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
- Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
- Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
- Ghilardi, N. et al. (2002) J. Biol. Chem 277:16831.
- Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
- Takaoka, A. et al. (2005) Eur. J. Pharmacol. 516:180.
Citations for Recombinant Human IL-31 Protein
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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miRNA551b-3p Activates an Oncostatin Signaling Module for the Progression of Triple-Negative Breast Cancer
Authors: D Parashar, A Geethadevi, MR Aure, J Mishra, J George, C Chen, MK Mishra, A Tahiri, W Zhao, B Nair, Y Lu, LS Mangala, C Rodriguez-, G Lopez-Bere, AKS Camara, M Liang, JS Rader, R Ramchandra, M You, AK Sood, VN Kristensen, GB Mills, S Pradeep, P Chaluvally
Cell Rep, 2019-12-24;29(13):4389-4406.e10.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
Regulation of IL-17A responses in human airway smooth muscle cells by Oncostatin M.
Authors: Kwofie K, Scott M, Rodrigues R, Guerette J, Radford K, Nair P, Richards C
Respir Res, 2015-02-07;16(0):14.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Interleukin 31 mediates MAP kinase and STAT1/3 activation in intestinal epithelial cells and its expression is upregulated in inflammatory bowel disease.
Authors: Dambacher J, Beigel F, Seiderer J, Haller D, Goke B, Auernhammer CJ, Brand S
Gut, 2007-04-20;56(9):1257-65.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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