Recombinant Human IL-32 beta Protein, CF Summary
Product Specifications
| MNHKVHHHHHH | Human IL-32 beta (Met1-Lys188) Accession # NP_001012649 |
| N-terminus | C-terminus |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
6769-IL
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and CHAPS. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: IL-32 beta
Interleukin 32 (IL‑32) is an N‑glycosylated cytokine that is up-regulated by inflammatory stimulation in monocytes, NK cells, epithelial cells, and vascular endothelial cells as well as in activated T cells (1‑6). It cooperates with inflammatory stimuli to promote the expression of other proinflammatory molecules such as TNF‑ alpha, IL‑6, IL‑1 beta, IL‑1 alpha, and CXCL8/IL‑8 (5‑9). The longest of several IL‑32 splicing variants is the 234 amino acid gamma isoform which is also known as natural killer cell transcript 4 (NK4) (9). The 25 kDa beta isoform (IL‑32 beta ) lacks aa 19‑64 including a portion of the putative signal peptide. Neutrophil‑derived Proteinase 3 (PR3) cleaves IL‑32 alpha between Thr57 and Val58, a cleavage site that is retained in other IL‑32 isoforms (10). The alpha, beta, gamma, delta, epsilon, and zeta isoforms show different degrees of antiviral activity against influenza virus replication with IL‑32 gamma being the most potent (11). IL‑32 is highly expressed by colonic epithelial cells in inflammatory bowel disease and Crohn’s disease, rheumatoid arthritis synovium, and ductal epithelial cells in chronic pancreatitis and pancreatic cancer (6, 12‑14). IL‑32 inhibits HIV‑1 replication in vitro, and it is elevated in the serum of HIV‑1 patients (15, 16).
- Netea, M.G. et al. (2006) PloS Med. 3:e277.
- Nold-Petry, C.A. et al. (2009) Proc. Natl. Acad. Sci. 106:3883.
- Li, W. et al. (2009) Eur. J. Immunol. 39:1019.
- Goda, C. et al. (2006) Int. Immunol. 18:233.
- Choi, J.-D. et al. (2009) Immunology 126:535.
- Shoda, H. et al. (2006) Arthritis Res. Ther. 8:R166.
- Netea, M.G. et al. (2005) Proc. Natl. Acad. Sci. 102:16309.
- Hong, J. et al. (2010) Cytokine 49:171.
- Kim, S.-H. et al. (2005) Immunity 22:131.
- Novick, D. et al. (2006) Proc. Natl. Acad. Sci. 103:3316.
- Li, W. et al. (2010) J. Immunol. 185:5056.
- Shioya, M. et al. (2007) Clin. Exp. Immunol. 149:480.
- Joosten, L.A.B. et al. (2006) Proc. Natl. Acad. Sci. 103:3298.
- Nishida, A. et al. (2009) J. Biol. Chem. 284:17868.
- Rasool, S.T. et al. (2008) Immunol. Lett. 117:161.
- Nold, M.F. et al. (2008) J. Immunol. 181:557.
Citations for Recombinant Human IL-32 beta Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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MHC-I upregulation safeguards neoplastic T cells in the skin against NK cell-mediated eradication in mycosis fungoides
Authors: Chang, YT;Prompsy, P;Kimeswenger, S;Tsai, YC;Ignatova, D;Pavlova, O;Iselin, C;French, LE;Levesque, MP;Kuonen, F;Bobrowicz, M;Brunner, PM;Pascolo, S;Hoetzenecker, W;Guenova, E;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
IL-32 induces epithelial-mesenchymal transition by triggering endoplasmic reticulum stress in A549 cells
Authors: L Gong, G Liu, H Zhu, C Li, P Li, C Liu, H Tang, K Wu, J Wu, D Liu, X Tang
BMC Pulm Med, 2020-10-23;20(1):278.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
M-CSF inhibits anti-HIV-1 activity of IL-32, but they enhance M2-like phenotypes of macrophages.
Authors: Osman A, Bhuyan F, Hashimoto M, Nasser H, Maekawa T, Suzu S
J Immunol, 2014-04-18;192(11):5083-9.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay -
Role of interleukin-32 in Helicobacter pylori-induced gastric inflammation.
Authors: Sakitani K, Hirata Y, Hayakawa Y, Serizawa T, Nakata W, Takahashi R, Kinoshita H, Sakamoto K, Nakagawa H, Akanuma M, Yoshida H, Maeda S, Koike K
Infect Immun, 2012-08-13;80(11):3795-803.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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