Recombinant Human IL-34 His-tag Protein, CF

Catalog #: 11449-IL Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11449-IL-010
11449-IL-050
11449-IL-250
11449-IL-01M

Recombinant Human IL‑34 His-tag Protein Bioactivity.

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Product Details

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-34 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to induce CCL2/MCP-1 secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 15.0-150 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-34 protein
Asn21-Val193, with a C-terminal 6-His tag

Accession #

AAH29804.1

N-terminal Sequence
Analysis

Asn21

Predicted Molecular Mass

21 kDa

SDS-PAGE

19-25 kDa, under reducing conditions.

Product Datasheets

11449-IL

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11449-IL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution	Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity

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Measured by its ability to induce CCL2/MCP-1 secretion by human peripheral blood mononuclear cells (PBMC). The ED₅₀ for this effect is 15.0-150 ng/mL.

SDS-PAGE

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2 μg/lane of Recombinant Human IL‑34 His-tag Protein (Catalog # 11449-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19‑25 kDa, under reducing conditions.

Reconstitution Calculator

Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N‑linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit‑macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

References