Home / IL-7R alpha/CD127 / Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF

Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF

Catalog #: 306-IR
9 Citations 3 Reviews Datasheet / COA / SDS
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306-IR-050
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Citations (9)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑7 R alpha /CD127 Fc Chimera (0.25 µg/mL, 100 µL/well) is immobilized on a goat anti‑human IgG Fc antibody (Catalog # G-102-C) coated plate, the concentration of recombinant human IL-7 that produces 50% of the optimal binding response is found to be 7.5-60 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-7R alpha/CD127 protein
Human IL-7 R alpha
(Glu21-Lys261)
Accession # BAG62793.1		 IIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
BAG62793.1
N-terminal Sequence
Analysis
Glu21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
54.5 kDa (monomer)
SDS-PAGE
80-90 kDa, reducing conditions

Product Datasheets

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306-IR

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

306-IR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

References
  1. Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
  2. Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193.
  3. Goodwin, R.G. et al. (1990) Cell 60:941.
  4. Noguchi, M. et al. (1993) Science 262:1877.
  5. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
  7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
  8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
  10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
  13. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
  15. Jahn, T. et al. (2007) Blood 110:1840.
  16. Lai, L. et al. (2006) Blood 107:1776.
Long Name
Interleukin 7 Receptor alpha
Entrez Gene IDs
3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)
Alternate Names
CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha

Citations for Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Interleukin-7 dependent non-classical monocytes and CD40 expression are affected in children with type 1 diabetes
    Authors: P Hehenkamp, M Hoffmann, S Kummer, C Reinauer, C Döing, K Förtsch, J Enczmann, V Balz, E Mayatepek, T Meissner, M Jacobsen, J Seyfarth
    European Journal of Immunology, 2021-11-01;0(0):.
    Applications: ELISA Standard
  2. Context-specific regulation of surface and soluble IL7R expression by an autoimmune risk allele
    Authors: H Al-Mossawi, N Yager, CA Taylor, E Lau, S Danielli, J de Wit, J Gilchrist, I Nassiri, EA Mahe, W Lee, L Rizvi, S Makino, J Cheeseman, M Neville, JC Knight, P Bowness, BP Fairfax
    Nat Commun, 2019-10-08;10(1):4575.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Standard
  3. IL7RA haplotypes differentially affect soluble IL-7 receptor and IL-7 serum concentrations in children with type 1 diabetes
    Authors: J Seyfarth, C Lundtoft, K Förtsch, H Ahlert, J Rosenbauer, C Baechle, M Roden, RW Holl, E Mayatepek, S Kummer, T Meissner, M Jacobsen
    Pediatr Diabetes, 2018-03-13;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis
    Authors: C Lundtoft, A Afum-Adjei, J Rimpler, K Harling, N Nausch, M Kohns, E Adankwah, F Lang, L Olbrich, E Mayatepek, E Owusu-Dabo, M Jacobsen
    PLoS Pathog., 2017-06-05;13(6):e1006425.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Reduced plasma levels of soluble interleukin-7 receptor during graft-versus-host disease (GVHD) in children and adults.
    Authors: Poiret, Thomas, Rane, Lalit, Remberger, Mats, Omazic, Birgitta, Gustafsson-Jernberg, Asa, Vudattu, Nalini K, Ahmed, Raija, Ernberg, Ingemar, Winiarski, Jacek, Magalhaes, Isabelle, Ringden, Olle, Maeurer, Markus
    BMC Immunol, 2014-06-19;15(0):25.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA (Standard)
  6. IL-7 Restores Lymphocyte Functions in Septic Patients.
    Authors: Venet F, Foray A, Villars-Mechin A, Malcus C, Poitevin-Later F, Lepape A, Monneret G
    J Immunol, 2012-10-10;189(10):5073-81.
    Applications: ELISA (Standard)
  7. Decreased systemic IL-7 and soluble IL-7Ralpha in multiple sclerosis patients.
    Genes Immun, 2012-08-23;13(7):587-92.
    Applications: ELISA (Standard)
  8. Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation.
    Authors: Faller EM, Sugden SM, McVey MJ
    J. Immunol., 2010-07-26;185(5):2854-66.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay
  9. Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
    Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
    J. Immunol., 2009-06-15;182(12):7389-97.
    Applications: ELISA (Standard)

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Reviews for Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF

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Recombinant Human IL-7R alpha/CD127 Fc Chimera Protein, CF
By Anonymous on 09/23/2018
Application: Binding assay/Protein-protein interaction
Recombinant Human IL-7 R alpha/CD127 Fc Chimera Protein, CF
By Anonymous on 08/20/2018
Application: In vitro bioactivity in cell culture
Recombinant Human IL-7 R alpha/CD127 Fc Chimera Protein, CF
By Lihong McAleer on 12/01/2017
Application: Binding assay/Protein-protein interaction