Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein, CF New

Catalog #: AVI9949 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AVI9949-050

Biotinylated Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein Binding Activity.

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human IL1RAPL1 Fc Chimera Avi-tag (Catalog # AVI9949) binds Recombinant Human PTPRD Fc Chimera (Catalog # 9995-PR) with an ED₅₀ of 1.50-20.0 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human IL1RAPL1 protein

Human IL-1RAPL1 (Leu19-Thr357) Accession # Q9NZN1.2	GGIEGRMD	Human IgG₁ (Pro100-Lys330)	Avitag
N-terminus			C-terminus

N-terminal Sequence
Analysis

Leu19

Structure / Form

Disulfide-linked homodimer, Biotinylated via Avi-tag

Predicted Molecular Mass

67 kDa

SDS-PAGE

80-90 kDa, under reducing conditions

Product Datasheets

AVI9949

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI9949

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

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Biotinylated Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein (Catalog # AVI9949) binds Recombinant Human PTPRD Fc Chimera (9995-PR) with an ED₅₀ of 1.50-20.0 ng/mL.

SDS-PAGE

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2 μg/lane of Biotinylated Recombinant Human IL1RAPL1 Fc Chimera Avi-tag Protein (Catalog # AVI9949) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-90 kDa and 160-180 kDa, respectively.

Reconstitution Calculator

Background: IL1RAPL1

Interleukin 1 receptor accessory protein-like 1 (IL1RAPL1), also known as Oligophenin-4 (OPHN4) and three immunoglobulin domain containing IL-1 receptor-related 2 (TIGIRR-2) (1), is a member of the IL-1 receptor superfamily. IL1RAPL1 is a single pass type I membrane protein which contains an N-terminal signal peptide (aa 1-18 ), three extracellular immunoglobulin-like domains (aa 19-350), a transmembrane domain (aa 358-378 ), an intracellular Toll/IL-1R domain (aa 403-562), and a long C-terminal tail which interacts with multiple signaling molecules (aa 549-644 ) (2). High expression levels of IL1RAPL1 was found in post-natal hippocampus, and its expression is upregulated by neuronal activity (3). The extracellular domain of IL1RAPL1 can mediate synapse formation through trans-synaptic interaction with PTPRD (4, 5). In neurons, IL1RAPL1 interacts with PSD-95, a major scaffolding protein of excitatory synapses, and modulates its synaptic localization by regulating JNK activity and PSD-95 phosphorylation (3). Mutation or deletion of IL1RAPL1 gene is associated with non-syndromic intellectual disability and autism spectrum disorder (5). Human IL1RAPL1 shares 98% and 99% aa sequence identity with mouse and rat IL1RAPL1, respectively. Our Avi-tag Biotinylated human IL1RAPL1 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
Bahi, N. et al. (2003) Hum. Mol. Gen. 12:1415.
Pavlowsky, A. et al. (2010) Curr. Biol. 20:103.
Yoshida, T. et al. (2011) J. Neurosci. 31:13485.
Ramos-Brossier, M. et al. (2015) Hum Mol Genet. 24:1106.

Long Name

Interleukin 1 Receptor Accessory Protein-like 1

Entrez Gene IDs

11141 (Human); 331461 (Mouse); 317553 (Rat)

Alternate Names

IL1R8IL1RAPL; IL-1R9; IL1RAPL1; IL-1RAPL1; IL-1-RAPL-1; IL-1RAPL-1; IL1RAPL-1; interleukin 1 receptor accessory protein-like 1; interleukin 1 receptor-8; interleukin-1 receptor accessory protein-like 1; mental retardation, X-linked 10; MRX10; MRX21; MRX34; oligophrenin-4; OPHN4mental retardation, X-linked 21; Three immunoglobulin domain-containing IL-1 receptor-related 2; TIGIRR-2; TIGIRR-2mental retardation, X-linked 34; X-linked interleukin-1 receptor accessory protein-like 1