Recombinant Human Inhibin A Protein Summary
Product Specifications
Ser233-Ile366 (alpha chain) & Gly311-Ser426 (beta chain)
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
8506-AB
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 250 μg/mL in 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
8506-AB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl. |
| Reconstitution | Reconstitute at 250 μg/mL in 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Scientific Data
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Recombinant Human Inhibin A (Cat# 8506-AB) neutralizes Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 3-18 ng/mL.
Reconstitution Calculator
Background: Inhibin A
Inhibin A is a member of the TGF-beta superfamily of proteins (1). Mature Inhibin A is a disulfide-linked dimer composed of alpha and beta subunits (1). Inhibin A shares a common alpha subunit with the closely related protein Inhibin B but has a unique beta subunit ( beta A) (2, 3). The mature alpha subunit of human Inhibin A has a predicted molecular weight of 18 kDa and shares 80% amino acid (aa) sequence identity with the mouse and rat orthologs (2, 4). The mature beta subunit of this human protein has a predicted molecular weight of 13 kDa and shares 100% aa sequence identity with the mouse and rat orthologs. Inhibin binds and antagonizes ActRIIA and ActRIIB in complex with the TGF-beta RIII and/or IGSF1 co-receptors and subsequently acts to suppress Activin-induced Follicle Stimulating Hormone (FSH) secretion (1, 5-7). Inhibins are produced by gonadal cells in both males and females (8). They are thought to be involved in the regulation of gametogenesis, and embryonic and fetal development (8, 9). Elevated concentrations of Inhibins are associated with pregnancy, preeclampsia, and ovarian cancer, and Inhibin A levels are typically measured during prenatal screening for Down’s syndrome (10-14).
- Phillips, D.J. and T.K. Woodruff (2004) Growth Factors 22:13.
- Mason, A.J. et al. (1986) Biochem. Biophys. Res. Commun. 135:957.
- Ying, S.Y. (1987) Proc. Soc. Exp. Biol. Med. 186:253.
- Mayo, K.E. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5849.
- Lewis, K.A. et al. (2000) Nature 404:411.
- Martens, J.W. et al. (1997) Endocrinology 138:2928.
- Chapman, S.C. et al. (2002) Mol. Cell. Endocrinol. 196:79.
- de Kretser, D.M. and D.M. Robertson (1989) Biol. Reprod. 40:33.
- Knight, P.G. et al. (2012) Mol. Cell. Endocrinol. 359:53.
- Walentowicz, P. et al. (2014) PLoS One 9:e90575.
- Kondi-Pafiti, A. et al. (2013) Clin. Exp. Obstet. Gynecol. 40:109.
- Kuijper, E.A. et al. (2013) Reprod. Biomed. Online 27:33.
- Carty, D.M. et al. (2008) Trends Cardiovasc. Med. 18:186.
- Wald, N.J. et al. (1996) Prenat. Diagn. 16:143.
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