Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF
Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF Summary
Product Specifications
| Human Integrin alpha 3 (Phe33-Glu991) Accession # P26006 |
acidic tail |
| Human Integrin beta 1 (Glu21-Asp728) Accession # P05556 |
basic tail |
| N-terminus | C-terminus |
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
2840-A3
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: Integrin alpha 3 beta 1/VLA-3
VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1-3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. The human beta 1/CD29 subunit is a 130-140 kDa type I transmembrane glycoprotein (8). Unlike the alpha -subunit, it does not undergo proteolytic cleavage. The molecule contains a 708 aa extracellular region, a 23 aa TM segment, and a 47 aa cytoplasmic domain. The extracellular region contains one von Willebrand factor-like A domain and four cysteine-rich repeats. The cytoplasimc domain shows five alternate splice forms (A, B, C1, C2 and D), all which vary by 12 to 48 amino acids (9, 10). Although the functions of all are unclear, the B isoform appears to be dominant negative to the A isoform (11). Human beta 1 extracellular domain is 92%, 94%, 93% and 96% aa identical to the extracellular regions of beta 1 in rat, bovine, mouse and feline, respectively. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (12).
- Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
- Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
- Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
- Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
- de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
- Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
- Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
- Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
- Svineng, G. et al. (1998) Biochem. J. 330:1255.
- van der Flier, A. et al. (1995) FEBS Lett. 369:340.
- Balzac, F. et al. (1994) J. Cell Biol. 127:557.
- Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.
Citations for Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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A recombinant technique for mapping functional sites of hetero-trimeric collagen helices: collagen IV CB3 fragment as a prototype for integrin binding
Authors: Boudko, SP;Konopka, EH;Kim, W;Taga, Y;Mizuno, K;Springer, TA;Hudson, BG;Moy, TI;Lin, FY;
The Journal of biological chemistry
Species: N/A
Sample Types: Protein, Small Molecule
Applications: Bioassay -
The effects of inhibition and siRNA knockdown of collagen-binding integrins on human umbilical vein endothelial cell migration and tube formation
Authors: EJ Hunter, SW Hamaia, PS Kim, JM Malcor, RW Farndale
Scientific Reports, 2022-12-14;12(1):21601.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture -
An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family
Authors: C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse
PLoS ONE, 2022-09-15;17(9):e0274667.
Species: Human
Sample Types: Cell Lysates
Applications: ELISA Capture -
Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist
Authors: KH Koh, Y Cao, S Mangos, NJ Tardi, RR Dande, HW Lee, B Samelko, MM Altintas, VP Schmitz, H Lee, K Mukherjee, V Peev, DJ Cimbaluk, J Reiser, E Hahm
J. Clin. Invest., 2019-03-18;129(4):1713-1726.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR -
Integrin alpha2beta1 mediates tyrosine phosphorylation of vascular endothelial cadherin induced by invasive breast cancer cells.
Authors: Haidari M, Zhang W, Caivano A, Chen Z, Ganjehei L, Mortazavi A, Stroud C, Woodside D, Willerson J, Dixon R
J Biol Chem, 2012-07-25;287(39):32981-92.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
FAQs
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What is the amino acid sequence of the acidic and basic tails?
Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
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