Recombinant Human ITIH3 His-tag Protein, CF

Catalog #: 10427-IT Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
10427-IT-050

Recombinant Human ITIH3 His-tag Protein SDS-PAGE

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human ITIH3 His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human ITIH3 is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human TSG‑6 (Catalog # 2104-TS) that produces 50% of the optimal binding response is 1-10 μg/mL

Source

Chinese Hamster Ovary cell line, CHO-derived human ITIH3 protein
Ser34-Asp651, with a C-terminal 6-His tag

Accession #

Q06033.2

N-terminal Sequence
Analysis

Ser34

Predicted Molecular Mass

70 kDa

SDS-PAGE

81-98 kDa, under reducing conditions

Product Datasheets

10427-IT

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10427-IT

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution	Reconstitute at 500 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE

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2 μg/lane of Recombinant Human ITIH3 His-tag Protein (Catalog # 10427-IT) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at approximately 86 and 93 kDa under reducing conditions.

Reconstitution Calculator

Background: ITIH3

Inter-alpha-trypsin inhibitor heavy chain 3 (ITIH3) is a heavy chain (HC) member of the ITIH family that is synthesized in the liver and circulates in the plasma (1). ITIH3 contains a signal sequence, propeptide, and a conserved von-Willebrand type A domain similar to other HCs in the family. The ITIH3 c-termini is trimmed to reveal a c-terminal aspartic acid residue that enables crosslinking of the HC to chondroitin sulfate (CS) (1,2). ITIH3 represents the single HC3 component of pre-a-trypsin inhibitor (PaI). PaI is a protease inhibitor involved in extracellular matrix stabilization that is composed of a single subunit of HC3 and bikunin, which are linked together via a CS moiety (1,3). ITIH3 can alternatively be associated with hyaluronan (HA) to form the Serum-derived hyaluronan associated protein (SHAP)-hyaluronan (HA) complex known to assist in stabilizing HA-rich extracellular matrices in the context of inflammatory processes and ovulation (3,4). ITIH3 is up-regulated in acute inflammation via interleukins and PaI is considered a positive acute-phase protein (1,5). ITIH3 is implicated as a diagnostic and prognostic marker in several diseases including colorectal cancer (6), gastric cancer (7), pancreatic cancer (8), and Sjogrens's syndrome with rheumatoid arthritis (9). ITIH3 has been shown to increase cell attachment and reduce the number of metastases in an antitumoral role (10). Recombinant Human ITIH3 activity is measured though binding to Tumor necrosis factor-stimulated gene-6 (TSG-6). TSG-6 has high affinity for HC and plays an important role in the transfer of HC to HA matrix by forming a TSG-6:HC complex intermediate required for the transfer (4,11).

References

Salier, J.P. et al.(1996) Biochem. J. 315:1.
Bourguignon, J. et al. (1993) Eur. J. Biochem. 212:771.
Zhuo, L. et al. (2004) J. Biol. Chem. 279:38079.
Rugg. M.S. et al. (2005) J. Biol. Chem. 280:25674.
Daveau, M. et al. (1993) Biochem. J. 292:485.
Jiang, X. et al. (2019) Dis. Markers. 2019:5069614.
Chong, P.K. et al. (2010) J. Proteome Res. 9:3671.
Liu, X. et al. (2017) Br. J. Cancer 117:1846.
Liao, C.C. et al. (2016) J. Proteomics. 141:1.
Paris, S. et al. (2002) Int. J. Cancer 97:615.
Baranova, N.S et al. (2013) J. Biol. Chem. 288:29642.

Long Name

Inter-Alpha-Trypsin Inhibitor Heavy Chain 3

Entrez Gene IDs

3699 (Human); 16426 (Mouse)

Alternate Names

H3 polypeptide; H3P; inter-alpha (globulin) inhibitor H3; inter-alpha (globulin) inhibitor, H3 polypeptide; inter-alpha-inhibitor heavy chain 3; inter-alpha-trypsin inhibitor heavy chain H3; ITI heavy chain H3; ITIH3; ITI-HC3; serum-derived hyaluronan-associated protein; SHAP