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Recombinant Human Jagged 2 Fc Chimera Protein, CF

Catalog #: 1726-JG
14 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
1726-JG-050
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Citations (14)
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human Jagged 2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Jagged 2 protein
Human Jagged 2
(Met27-Leu1082)
Accession #AAB61285		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
AAB84215
N-terminal Sequence
Analysis
Met27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
138.7 kDa (monomer)
SDS-PAGE
165-175 kDa, reducing conditions

Product Datasheets

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1726-JG

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1726-JG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Jagged 2

Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1 - 5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor (SwissProt # Q9Y219). It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421 - 461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2-week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).

References
  1. Shawber, C. et al. (1996) Dev. Biol. 180:370.
  2. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
  3. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
  4. Schickwann, T. et al. (2000) Blood 96:950.
  5. DeHart, S. et al. (2005) Blood 105:3521.
  6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.
Entrez Gene IDs
3714 (Human); 16450 (Mouse); 29147 (Rat)
Alternate Names
HJ2; JAG2; Jagged 2; Jagged2; protein jagged-2; SER2

Citations for Recombinant Human Jagged 2 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. DLL4-Notch3-WNT5B axis mediates bi-directional pro-metastatic crosstalk between melanoma and lymphatic endothelial cells
    Authors: Alve, S;Gramolelli, S;Jukonen, J;Juteau, S;Pink, A;Manninen, AA;Hänninen, S;Monto, E;Lackman, MH;Carpén, O;Saharinen, P;Karaman, S;Vaahtomeri, K;Ojala, PM;
    JCI insight
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. ILC2s induce adaptive Th2-type immunity in different stages of tuberculosis through the Notch-GATA3 pathway
    Authors: Q Li, J Zhang, L Ma, C Wu, J Zhao, W Guan, X Li, X Yang, F Wen
    International immunopharmacology, 2021-11-30;0(0):108330.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Jagged Ligands Enhance the Pro-Angiogenic Activity of Multiple Myeloma Cells
    Authors: MT Palano, D Giannandre, N Platonova, G Gaudenzi, M Falleni, D Tosi, E Lesma, V Citro, M Colombo, I Saltarella, R Ria, N Amodio, E Taiana, A Neri, G Vitale, R Chiaramont
    Cancers (Basel), 2020-09-11;12(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2
    Authors: S Kakuda, RK Lopilato, A Ito, RS Haltiwange
    J. Biol. Chem., 2020-08-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Tumour-associated neutrophils orchestrate intratumoural IL-8-driven immune evasion through Jagged2 activation in ovarian cancer
    Authors: M Yang, G Zhang, Y Wang, M He, Q Xu, J Lu, H Liu, C Xu
    Br. J. Cancer, 2020-08-11;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Skip is essential for Notch signaling to induce Sox2 in cerebral arteriovenous malformations
    Authors: D Zhang, X Qiao, L Wang, L Zhang, J Yao, X Wu, T Yu, KI Boström, Y Yao
    Cell. Signal., 2020-01-10;68(0):109537.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Specific NOTCH1 antibody targets DLL4-induced proliferation, migration, and angiogenesis in NOTCH1-mutated CLL cells
    Authors: M López-Guer, S Xargay-Tor, P Fuentes, J Roldán, B González-F, L Rosich, E Silkensted, MJ García-Leó, E Lee-Vergés, N Giménez, A Giró, M Aymerich, N Villamor, J Delgado, A López-Guil, XS Puente, E Campo, ML Toribio, D Colomer
    Oncogene, 2019-10-15;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Multiple myeloma exploits Jagged1 and Jagged2 to promote intrinsic and bone marrow-dependent drug resistance
    Authors: M Colombo, S Garavelli, M Mazzola, N Platonova, D Giannandre, R Colella, L Apicella, M Lancellott, E Lesma, S Ancona, MT Palano, M Barbieri, E Taiana, E Lazzari, A Basile, M Turrini, A Pistocchi, A Neri, R Chiaramont
    Haematologica, 2019-10-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Anti-Leukemia Effects of Notch-Mediated Inhibition of Oncogenic PLK1 in B-Cell Acute Lymphoblastic Leukemia
    Authors: S Kannan, MJL Aitken, SM Herbrich, LS Golfman, MG Hall, DH Mak, JK Burks, G Song, M Konopleva, CG Mullighan, J Chandra, PA Zweidler-M
    Mol. Cancer Ther., 2019-06-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Crosstalk between BMP and Notch Induces Sox2 in Cerebral Endothelial Cells
    Authors: X Wu, J Yao, L Wang, D Zhang, L Zhang, EX Reynolds, T Yu, KI Boström, Y Yao
    Cells, 2019-06-06;8(6):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  11. JAG2 signaling induces differentiation of CD14+ monocytes into Langerhans cell histiocytosis-like cells
    Authors: R Schwentner, G Jug, MO Kauer, T Schnöller, P Waidhofer-, W Holter, C Hutter
    J. Leukoc. Biol., 2018-10-08;105(1):101-111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  12. Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.
    Authors: Diaz B, Yuen A, Iizuka S, Higashiyama S, Courtneidge S
    J Cell Biol, 2013-04-15;201(2):279-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. The alarmin IL-33 is a notch target in quiescent endothelial cells.
    Authors: Sundlisaeter E, Edelmann R, Hol J, Sponheim J, Kuchler A, Weiss M, Udalova I, Midwood K, Kasprzycka M, Haraldsen G
    Am J Pathol, 2012-07-16;181(3):1099-111.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Derivation of cerebellar neurons from human pluripotent stem cells.
    Authors: Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M, Bhattacharya S
    Curr Protoc Stem Cell Biol, 2012-03-01;20(0):1H.51.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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