Recombinant Human L-Selectin/CD62L His-tag Protein, CF

Catalog #: 11374-LS Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
11374-LS-100

Recombinant Human L‑Selectin/CD62L His-tag Protein Binding Activity.

2 Images

All L-Selectin/CD62L Proteins and Enzymes

Product Details

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human L-Selectin/CD62L His-tag Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Human L-Selectin/CD62L Antibody (Catalog # AF728) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human L-Selectin/CD62L His-tag (Catalog # 11374-LS) binds with an ED₅₀ of 0.180-1.80 ng/mL.

Source

Chinese Hamster Ovary cell line, CHO-derived human L-Selectin/CD62L protein
Trp52-Leu332, with a C-terminal 6-His tag

Accession #

EAW90856.1

N-terminal Sequence
Analysis

Trp52

Predicted Molecular Mass

32 kDa

SDS-PAGE

65-75 kDa, under reducing conditions.

Product Datasheets

11374-LS

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11374-LS

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity

View Larger

When Human L-Selectin/CD62L Antibody (AF728) is immobilized at 0.1 µg/mL (100 µL/well), Recombinant Human L-Selectin/CD62L His-tag (Catalog # 11374-LS) binds with an ED₅₀ of 0.180-1.80 ng/mL.

SDS-PAGE

View Larger

2 μg/lane of Recombinant Human L‑Selectin/CD62L His-tag Protein (Catalog # 11374-LS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 65-75 kDa.

Reconstitution Calculator

Background: L-Selectin/CD62L

L-Selectin, also known as Leukocyte adhesion molecule 1 (LAM-1) and CD62L, is a type-1 cell surface glycoprotein and cell adhesion molecule of the Selectin family (1). In humans, there are 3 Selectins, P, E, and L, and they are Ca2+ dependent lectins that help mediate the initial adhesive step during inflammation and immune surveillance (2). Mature L-Selectin consists of an extracellular domain (ECD) with a C-type lectin domain and an epidermal growth factor (EGF)-like domain, a transmembrane domain, and a short cytoplasmic domain. Within the ECD, human L-selectin shares 76% and 78% amino acid sequence identity with mouse and rat L-selectin, respectively. Several isoforms arising from alternative splicing have been reported, some with potential therapeutic implications (2,3). L-selectin is constitutively expressed on a wide variety of leukocytes and plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites (1-4). Acting in cooperation with P-Selectin and E-Selectin, L-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium (5). This initial interaction, also involving ICAM‑1 and VCAM-1, leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue (6). L-selectin function is required for normal Treg cell migration and over expression might be result in reduced tumor growth (7). Several studies have reported that levels of L-Selectin may be elevated or lowered in subjects with a variety of conditions, such as in Alzheimer's disease or rheumatoid arthritis (3, 5).

References

Ivetic, A. et al. (2019) Front. Immunol. 10:1068.
Grailer, J.J. et al. (2009) J. Dermatol sci. 56:141.
Hirata, T. et al. (2015) Biochem Biophys Res. Commun. 462:371.
Wedepohl, S. et al. (2012) Euro. J. Cell Biol. 91:257.
Ivetic, A. et al. (2013) Inter. J. Biochem. Cell Biol. 45:550.
Granger, D.N. and Senchenkova, E. (2010) Inflammation and the Microcirculation. San Rafael (CA): Morgan & Claypool Life Sciences Chapter 7.
Watson, H.A. et al. (2019) Frontiers in immunology 10:1321.

Entrez Gene IDs

6402 (Human); 20343 (Mouse); 29259 (Rat); 102142895 (Cynomolgus Monkey)

Alternate Names

CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1