Recombinant Human LAMP-1/CD107a Protein, CF

Catalog #: 4800-LM Datasheet / COA / SDS

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4800-LM-050

R&D Systems Recombinant Proteins and Enzymes

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Recombinant Human LAMP-1/CD107a Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human LAMP1/CD107a is coated at 1 μg/mL, Recombinant Human Galectin‑3 (Catalog # 1154-GA) binds with an apparent K_D <25 nM.

Source

Mouse myeloma cell line, NS0-derived human LAMP-1/CD107a protein
Ala29-Met382 with a C-terminal 6-His tag

Accession #

P11279

N-terminal Sequence
Analysis

Ala29 & Met30

Predicted Molecular Mass

39.2 kDa

SDS-PAGE

100-110 kDa, reducing conditions

Product Datasheets

4800-LM

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4800-LM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: LAMP-1/CD107a

Lysosomal associated membrane protein 1 (LAMP1), also known as CD107a and LGP120, is an approximately 120 kDa transmembrane glycoprotein that is a major protein component of lysosomal membranes (1). Mature human LAMP1 consists of a 354 amino acid (aa) intralumenal domain (ECD), a 23 aa transmembrane segment, and a 12 aa cytoplasmic tail (2). Its lumenal domain is organized into two heavily N-glycosylated regions separated by a Ser/Pro-rich linker that carries a minor amount of O-linked glycosylation (2, 3). Within the lumenal domain, human LAMP1 shares approximately 64% aa sequence identity with mouse and rat LAMP1. The sorting of LAMP1 to lysosomes relies on a tyrosine motif in the cytoplasmic tail (3, 4). In cytotoxic T cells and mast cells, LAMP1 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine (5-7). LAMP1 is presented on the plasma membrane during the activation of NK cells, CD8⁺ T cells, mast cells, basophils, monocytes, and platelets (6-12). A glycoform of LAMP1 known as M150 is expressed on the surface of activated macrophages where it promotes T cell costimulation and a Th1 biased immune response (13). Exposure of epithelial cells to pathogenic Neisseria bacteria induces the redistribution of LAMP1 to the cell surface where it can be cleaved by the Neisseria IgA1 protease (14). LAMP1 is a native ligand for lectins Galectin-1 and Galectin-3 (15‑17).

References

Eskelinen, E.L. et al. (2003) Trends Cell Biol. 13:137.
Viitala, J. et al. (1988) Proc. Natl. Acad. Sci. 85:3743.
Carlsson, S.R. et al. (1988) J. Biol. Chem. 263:18911.
Rohrer, J. et al. (1996) J. Cell Biol. 132:565.
Peters, P.J. et al. (1991) J. Exp. Med. 173:1099.
Betts, M.R. et al. (2003) J. Immunol. Meth. 281:65.
Grutzkau, A. et al. (2004) Cytometry 61:62.
Alter, G. et al. (2004) J. Immunol. Meth. 294:15.
Tomescu, C. et al. (2009) J. Leukoc. Biol. 85:871.
Hennersdorf, F. et al. (2005) Cell Res. 15:325.
Kannan, K. et al. (1996) Cell. Immunol. 171:10.
Fabbraio, M. and R.L. Silverstein (1990) J. Biol. Chem. 265:18531.
Prasad, D.V.R. et al. (2002) J. Immunol. 169:1801.
Lin, L. et al. (1997) Mol. Microbiol. 24:1083.
Skrincosky, D.M. et al. (1993) Cancer Res. 53:2667.
Inohara, H. and Raz, A. (1994) Biochem. Biophys. Res. Commun. 201:1366.
Ohannesian D.W. et al. (1994) Cancer Res. 54:5992.

Long Name

Lysosome-associated Membrane Glycoprotein 1

Entrez Gene IDs

3916 (Human); 16783 (Mouse)

Alternate Names

CD107 antigen-like family member A; CD107a antigen; CD107a; LAMP1; LAMP-1; LAMPA; LGP120; lysosomal-associated membrane protein 1; lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1