Recombinant Human LDLR Protein

Catalog #: 2148-LD
9 Citations Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
2148-LD-025/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
2148-LD-025

R&D Systems Recombinant Proteins and Enzymes

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Product Details

Citations (9)

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Human LDLR Protein Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a competitive binding assay. When human LDL is immobilized at 1 μg/mL (100 μL/well), Recombinant Human LDL R inhibits 50% binding of biotinylated recombinant human LDL R (0.5 μg/mL) at the concentration range of 0.4-2 μg/mL.

Source

Mouse myeloma cell line, NS0-derived human LDL R protein
Ala22-Arg788, with a C-terminal 6-His tag

Accession #

Q59FQ1

N-terminal Sequence
Analysis

Ala22 & Asp193

Predicted Molecular Mass

85.6 kDa & 66.8 kDa

SDS-PAGE

125-140 kDa & 95-110 kDa, reducing conditions

Product Datasheets

2148-LD (with carrier)

Product Datasheet

COA

SDS

2148-LD/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2148-LD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

2148-LD/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: LDLR

The low density lipoprotein receptor (LDL R) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors (1-5). Members of the family are endocytic receptors, but can also co-regulate adjacent cell-surface signaling molecules (3, 4). Many proteins in the LDL R family are cleaved by extracellular proteases to release soluble forms to the circulation, and many of these soluble forms are active (1, 6). Mature LDL R is a 120-160 kDa (depending on glycosylation) type I transmembrane glycoprotein that contains cysteine-rich complement-like repeats (class A LDL domains), calcium-binding EGF repeats, and beta ‑propeller structures (class B LDL repeats) in the extracellular domain (ECD) (1-7). A membrane-proximal Ser/Thr-rich region shows extensive O-linked glycosylation (4, 8). A cytoplasmic NPxY motif links the LDL R to clathrin pits for endocytosis, and binds to select adaptor proteins (1, 4, 8). The human LDL R ECD shares 78%, 76%, 81% and 82% aa sequence identity with mouse, rat, bovine, and porcine LDL R, respectively. LDL R is constitutively and widely expressed. Its class A LDL domains near the N-terminus bind apoB and apoE, the apolipoproteins of low- and very low-density lipoproteins (LDL and VLDL), respectively (1, 2, 4, 9). Hepatocyte LDL R is responsible for endocytosis and clearing of most plasma LDL cholesterol (2, 9). At the low pH of the endocytic vesicle, it dissociates, allowing degradation of LDL and recycling of LDL R to the cell surface (1, 4). Lack of LDL R expression or function causes familial hypercholesterolemia (FH) (4, 9, 10). The protease PCSK9 (proprotein convertase subtilisin/kexin type 9) can also cause increased plasma cholesterol by promoting LDL R degradation rather than recycling to the cell surface (10-12). Soluble forms of approximately 140 kDa and 28 kDa are reported to be released by phorbol esters or interferons, respectively (6, 7)

References

Go, G.W. and A. Mani (2012) Yale J. Biol. Med. 85:19.
Ren, G. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1059.
Bujo, H. and Y. Saito (2006) Arterioscler. Thromb. Vasc. Biol. 26:1246.
Gent, J. and I. Braakman (2004) Cell. Mol. Life Sci. 61:2461.
Yamamoto, T. et al. (1984) Cell 39:27.
Begg, M.J. et al. (2004) Eur. J. Biochem. 271:524.
Fischer, D.G. et al. (1993) Science 262:250.
Stolt, P.C. and H.H. Bock (2006) Cell. Signal. 18:1560.
Defesche, J.C. (2004) Semin. Vasc. Med. 4:5.
De Castro-Oros, I. et al. (2010) Appl. Clin Genet. 3:53.
Zhang, D.W. et al. (2008) Proc. Natl. Acad. Sci. USA 105:13045.
Tavori, H. et al. (2013) Circulation 127:2403.

Long Name

Low Density Lipoprotein Receptor

Entrez Gene IDs

3949 (Human); 16835 (Mouse); 300438 (Rat); 396801 (Porcine); 102127361 (Cynomolgus Monkey)

Alternate Names

FH; FHC; LDL R; LDL receptor; LDLCQ2; LDLR; low density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3; low-density lipoprotein receptor

Related Research Areas

Citations for Recombinant Human LDLR Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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The low-density lipoprotein receptor and apolipoprotein E associated with CCHFV particles mediate CCHFV entry into cells
Authors: Ritter, M;Canus, L;Gautam, A;Vallet, T;Zhong, L;Lalande, A;Boson, B;Gandhi, A;Bodoirat, S;Burlaud-Gaillard, J;Freitas, N;Roingeard, P;Barr, JN;Lotteau, V;Legros, V;Mathieu, C;Cosset, FL;Denolly, S;
Nature communications
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Generation and Characterization of a Novel Small Biologic Alternative to Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Antibodies, DS-9001a, Albumin Binding Domain-Fused Anticalin Protein
Authors: Y Masuda, S Yamaguchi, C Suzuki, T Aburatani, Y Nagano, R Miyauchi, E Suzuki, N Yamamura, K Nagatomo, H Ishihara, K Okuno, F Nara, G Matschiner, R Hashimoto, T Takahashi, T Nishizawa
J. Pharmacol. Exp. Ther., 2018-02-20;365(2):368-378.
Species: Human
Sample Types: Recombinant Protein
Applications: Bioassay
Ruxolitinib and polycation combination treatment overcomes multiple mechanisms of resistance of pancreatic cancer cells to oncolytic vesicular stomatitis virus
Authors: SA Felt, GN Droby, VZ Grdzelishv
J. Virol., 2017-07-27;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor.
Authors: Amirache F, Levy C, Costa C, Mangeot P, Torbett B, Wang C, Negre D, Cosset F, Verhoeyen E
Blood, 2014-02-27;123(9):1422-4.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Mapping the binding region on the low density lipoprotein receptor for blood coagulation factor VIII.
Authors: Kurasawa J, Shestopal S, Karnaukhova E, Struble E, Lee T, Sarafanov A
J Biol Chem, 2013-06-10;288(30):22033-41.
Species: Human
Sample Types: Protein
Applications: Binding Assay
Characterization of proprotein convertase subtilisin/kexin type 9 (PCSK9) trafficking reveals a novel lysosomal targeting mechanism via amyloid precursor-like protein 2 (APLP2).
Authors: DeVay, Rachel M, Shelton, David L, Liang, Hong
J Biol Chem, 2013-02-19;288(15):10805-18.
Species: Human
Sample Types: Cell Lysates
Applications: Bioassay
Antibody-mediated disruption of the interaction between PCSK9 and the low-density lipoprotein receptor.
Authors: Duff CJ, Scott MJ, Kirby IT, Hutchinson SE, Martin SL, Hooper NM
Biochem. J., 2009-05-01;419(3):577-84.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance
Bovine lactoferrin inhibits Japanese encephalitis virus by binding to heparan sulfate and receptor for low density lipoprotein.
Authors: Chien YJ, Chen WJ, Hsu WL, Chiou SS
Virology, 2008-07-21;379(1):143-51.
Species: Hamster
Sample Types: Whole Cells
Applications: Bioassay
Effects of pH and low density lipoprotein (LDL) on PCSK9-dependent LDL receptor regulation.
Authors: Fisher TS, Lo Surdo P, Pandit S, Mattu M, Santoro JC, Wisniewski D, Cummings RT, Calzetta A, Cubbon RM, Fischer PA, Tarachandani A, De Francesco R, Wright SD, Sparrow CP, Carfi A, Sitlani A
J. Biol. Chem., 2007-05-10;282(28):20502-12.
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance, TR-FRET