Recombinant Human LECT2 Protein, CF

Recombinant Human LECT2 Protein, CF Summary

Product Specifications

Product Datasheets

Carrier Free

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: LECT2

LECT2 (leukocyte cell-derived chemotaxin-2), also known as Chondromodulin-II, is a neutrophil chemotactic protein predominantly expressed in the liver (1). It was first identified in the heparin-binding components extracted from fetal bovine epiphyseal cartilage (2). Human LECT2 cDNA encodes a 151 amino acid (aa) precursor that includes an 18 aa signal sequence (3). The mature human LECT2 is a 16 kDa secreted hepatic protein that has a putative peptidase-M23 domain (3, 4). Human LECT2 shares 87% and 86% aa sequence identity with mouse and rat LECT2, respectively. LECT2 stimulates the growth and matrix formation of chondrocytes in vitro (2, 5, 6). In MC3T3-E1 cells, it promotes proliferation but inhibits alkaline phosphatase activity (5, 6). In vivo study suggested LECT2 can directly suppress the development of type II collagen antibody–induced arthritis (4). Recent studies have shown that LECT2 is an important regulator of tumor growth during hepatocellular carcinoma development and progression; it inhibits the angiogenic effect of HUVECs in vitro and in vivo (7).